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Open AccessArticle

Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation

by Szu-Yuan Wu 1,2,3,4,5,†, Yu-Ting Chen 6,†, Guo-Yu Tsai 6, Fu-Yin Hsu 6 and Pai-An Hwang 6,7,*
1
Department of Food Nutrition and Health Biotechnology, College of Medical and Health Science, Asia University, Taichung 413, Taiwan
2
Division of Radiation Oncology, Lo-Hsu Medical Foundation, Lotung Poh-Ai Hospital, Yilan 265, Taiwan
3
Department of Radiation Oncology, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan
4
Big Data Center, Lo-Hsu Medical Foundation, Lotung Poh-Ai Hospital, Yilan 265, Taiwan
5
Department of Healthcare Administration, College of Medical and Health Science, Asia University, Taichung 413, Taiwan
6
Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan
7
Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2020, 18(3), 136; https://doi.org/10.3390/md18030136
Received: 3 February 2020 / Revised: 20 February 2020 / Accepted: 25 February 2020 / Published: 27 February 2020
(This article belongs to the Special Issue Marine Polysaccharides in Pharmaceutical Applications)
Radiation-induced fibrosis (RIF) occurs after radiation therapy in normal tissues due to excessive production and deposition of extracellular matrix proteins and collagen, possibly resulting in organ function impairment. This study investigates the effects of low-molecular-weight fucoidan (LMF) on irradiated NIH3T3 cells. Specifically, we quantified cellular metabolic activity, fibrosis-related mRNA expression, transforming growth factor beta-1 (TGF-β1), and collagen-1 protein expression, and fibroblast contractility in response to LMF. LMF pre + post-treatment could more effectively increase cellular metabolic activity compared with LMF post-treatment. LMF pre + post-treatment inhibited TGF-β1 expression, which mediates negative activation of phosphorylated Smad3 (pSmad3) and Smad4 complex formation and suppresses downstream collagen I accumulation. In addition, LMF pre + post-treatment significantly reduced actin-stress fibers in irradiated NIH3T3 cells. LMF, a natural substance obtained from brown seaweed, may be a candidate agent for preventing or inhibiting RIF. View Full-Text
Keywords: low-molecular-weight fucoidan; radiation-induced fibrosis; TGF-β1; collagen I; Smad pathway low-molecular-weight fucoidan; radiation-induced fibrosis; TGF-β1; collagen I; Smad pathway
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MDPI and ACS Style

Wu, S.-Y.; Chen, Y.-T.; Tsai, G.-Y.; Hsu, F.-Y.; Hwang, P.-A. Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation. Mar. Drugs 2020, 18, 136.

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