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Open AccessArticle

Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

1
Zhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
2
College of Animal Science, Zhejiang University, Hangzhou 310015, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2020, 18(2), 119; https://doi.org/10.3390/md18020119
Received: 11 December 2019 / Revised: 5 February 2020 / Accepted: 13 February 2020 / Published: 18 February 2020
(This article belongs to the Special Issue Anti-Microbial Compounds from Marine Sources)
Bacillus amyloliquefaciens-9 (GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark (Chiloscyllium plagiosum), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptides. However, the low fermentation production has hindered the development of GBacillus-9 as biological additives. In this study, the Plackett–Burman design and response surface methodology were used to optimize the fermentation conditions in a shake flask to obtain a higher yield and antibacterial activity of GBacillus-9. On the basis of the data from medium screening, M9 medium was selected as the basic medium for fermentation. The data from the single-factor experiment showed that sucrose had the highest antibacterial activity among the 10 carbon sources. The Plackett–Burman design identified sucrose, NH4Cl, and MgSO4 as the major variables altering antibacterial activity. The optimal concentrations of these compounds to enhance antibacterial activity were assessed using the central composite design. Data showed that sucrose, NH4Cl, and MgSO4 had the highest antibacterial activities at concentrations of 64.8, 1.84, and 0.08 g L−1, respectively. The data also showed that the optimal fermentation conditions for the antibacterial material production of GBacillus-9 were as follows: Inoculum volume of 5%, initial pH of 7.0, temperature of 36 °C, rotating speed of 180 rpm, and fermentation time of 10 h. The optimal fermentation medium and conditions achieved to improve the yield of antibacterial materials for GBacillus-9 can enhance the process of developing biological additives derived from GBacillus-9. View Full-Text
Keywords: antibacterial materials; Bacillus amyloliquefaciens; whitespotted bamboo shark; biological additive antibacterial materials; Bacillus amyloliquefaciens; whitespotted bamboo shark; biological additive
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Zhang, W.; Wei, L.; Xu, R.; Lin, G.; Xin, H.; Lv, Z.; Qian, H.; Shi, H. Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum). Mar. Drugs 2020, 18, 119.

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