Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Materials
3.2. Extract Preparation from Microalgae
3.3. Cellular Viability
3.4. Bio-Guided Fractionation and Isolation of Anti-Tumoral Compounds
3.5. Spectral and Chromatographic Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Position | Loliolide (1) | Epi-loliolide (2) | ||
---|---|---|---|---|
δC | δH, m, J (Hz) | δC | δH, m, J (Hz) | |
2 | 171.49 | - | 171.55 | - |
3 | 112.89 | 5.69 s, 1H | 113.24 | 5.71 s, 1H |
3a | 182.68 | - | 180.75 | - |
4 | 35.91 | - | 35.03 | - |
5 | 47.26 | 1.53 dd, 14.5, 3.3, 1H, α-Hax | 49.7 | 1.33 t, 12.8, 1H, β-Hax |
1.97 brd, 14.6, 1H, β-Heq | 2.04 brd, 12.8, 1H, α-Heq | |||
6 | 66.81 | 4.33 m, 1H, α-Heq | 65.03 | 4.13 tt, 11.5, 4.1, 1H, α-Hax |
7 | 45.56 | 1.79 m, 1H, α-Hax | 47.25 | 1.51 t, 11.9, 1H, β-Hax |
2.45 brd, 14.1, 1H, β-Heq | 2.54 brd, 11.8, 1H, α-Heq | |||
7a | 86.67 | - | 86.45 | - |
4α-Me | 30.63 | 1.27 s, 3H, Meeq | 25.04 | 1.26 s, 3H, Meax |
4β-Me | 26.45 | 1.46 s, 3H, Meax | 29.89 | 1.31 s, 3H, Meeq |
7a-Me | 26.96 | 1.78 s, 3H, β-Meax | 25.55 | 1.58 s, 3H, α-Meax |
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Gangadhar, K.N.; Rodrigues, M.J.; Pereira, H.; Gaspar, H.; Malcata, F.X.; Barreira, L.; Varela, J. Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea. Mar. Drugs 2020, 18, 567. https://doi.org/10.3390/md18110567
Gangadhar KN, Rodrigues MJ, Pereira H, Gaspar H, Malcata FX, Barreira L, Varela J. Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea. Marine Drugs. 2020; 18(11):567. https://doi.org/10.3390/md18110567
Chicago/Turabian StyleGangadhar, Katkam N., Maria João Rodrigues, Hugo Pereira, Helena Gaspar, F. Xavier Malcata, Luísa Barreira, and João Varela. 2020. "Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea" Marine Drugs 18, no. 11: 567. https://doi.org/10.3390/md18110567