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Open AccessArticle

Enhanced Lipid Production and Molecular Dynamics under Salinity Stress in Green Microalga Chlamydomonas reinhardtii (137C)

1
Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok 10330, Thailand
2
The Chemical Approaches for Food Applications Research Group, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok 10330, Thailand
3
Graduate School of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan
4
Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok 10330, Thailand
5
Thailand Institute of Scientific and Technological Research (TISTR), Khlong Luang, Pathum Thani 12120, Thailand
6
Graduate School of Environmental and Human Sciences, Meijo University, Nagoya 468-8502, Japan
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(8), 484; https://doi.org/10.3390/md17080484
Received: 31 July 2019 / Revised: 15 August 2019 / Accepted: 18 August 2019 / Published: 20 August 2019
Microalgal lipids are a source of valuable nutritional ingredients in biotechnological industries, and are precursors to biodiesel production. Here, the effects of salt-induced stresses, including NaCl, KCl, and LiCl stresses, on the production of lipid in green microalga Chlamydomonas reinhardtii (137c) were investigated. NaCl stress dramatically increased saturated fatty acids (SFAs), which accounted for 70.2% of the fatty acid methyl ester (FAMEs) under stress. In contrary, KCl stress led to a slight increase in SFAs (47.05%) with the remaining being polyunsaturated fatty acids (PUFAs) (45.77%). RT-PCR analysis revealed that the genes involved in FA biosynthesis, such as PDH2, ACCase, MAT and KAS2, were up-regulated by NaCl-induced stress. Conversely, the genes responsible for the Kennedy pathway were suppressed. The alteration of FA homeostasis was further assessed by overexpressing MAT, the enzyme responsible for the production of malonyl-ACP, a key building block for FA biosynthesis, in the cyanobacterium Synechococcus elongatus PCC 7942. Intracellular FA composition was affected, with a predominant synthesis of SFAs in transformed cells. Owing to the diversity and relative abundance of SFAs, monounsaturated fatty acid (MUFAs) and PUFAs enable the feasibility of using microorganisms as a source of microalgal lipids or valuable nutritional ingredients; salt-induced stress and expression of MAT are useful in providing precursors for enhanced lipid production. View Full-Text
Keywords: salt stress; lipid production; microalgae; malonyl-ACP; salinity salt stress; lipid production; microalgae; malonyl-ACP; salinity
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MDPI and ACS Style

Atikij, T.; Syaputri, Y.; Iwahashi, H.; Praneenararat, T.; Sirisattha, S.; Kageyama, H.; Waditee-Sirisattha, R. Enhanced Lipid Production and Molecular Dynamics under Salinity Stress in Green Microalga Chlamydomonas reinhardtii (137C). Mar. Drugs 2019, 17, 484.

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