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Mar. Drugs 2019, 17(3), 148; https://doi.org/10.3390/md17030148

High-Performance Thin-Layer Chromatography Hyphenated with Microchemical and Biochemical Derivatizations in Bioactivity Profiling of Marine Species

1
School of Pharmacy and Applied Science, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia
2
Department of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia
3
Department of Creative Arts and English, La Trobe University, Edwards Rd, Bendigo 3550, Australia
*
Author to whom correspondence should be addressed.
Received: 12 December 2018 / Revised: 17 February 2019 / Accepted: 25 February 2019 / Published: 3 March 2019
(This article belongs to the Special Issue Vascular Bioactivities of Marine Natural Products)
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Abstract

Marine organisms produce an array of biologically active natural products, many of which have unique structures that have not been found in terrestrial organisms. Hence, marine algae provide a unique source of bioactive compounds. The present study investigated 19 marine algae and one seagrass collected from Torquay beach, Victoria, Australia. High-performance thin-layer chromatography (HPTLC) hyphenated with microchemical (DPPH•, p-anisaldehyde, and Fast Blue B) and biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to evaluate antioxidant activity, presence of phytosterols and phenolic lipids, α-amylase and AChE inhibitory activities of extract components. Significant α-amylase and AChE inhibitory activities were observed in samples 2, 6, 8 and 10. Antioxidant activities in the samples were found to be correlated to phytosterol content (R2 = 0.78), but was not found to be related to either α-amylase or AChE inhibitory activities. α-Amylase inhibitory activities were correlated to AChE inhibition (R2 = 0.77) and attributed to the phytosterol content, based on the similar peak position in the chromatograms with the β-sitosterol chromatogram. Samples 1, 8, and especially sample 20, were found to contain phenolic lipids (alkyl resorcinol derivatives) with significant antioxidant activities. The results suggest that these marine species have a significant number of bioactive compounds that warrant further investigation. View Full-Text
Keywords: α-amylase inhibitors; antioxidants; cholinesterase inhibitors; effect-directed analysis (EDA); HPTLC; marine algae; phenolic lipids α-amylase inhibitors; antioxidants; cholinesterase inhibitors; effect-directed analysis (EDA); HPTLC; marine algae; phenolic lipids
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Agatonovic-Kustrin, S.; Kustrin, E.; Gegechkori, V.; Morton, D.W. High-Performance Thin-Layer Chromatography Hyphenated with Microchemical and Biochemical Derivatizations in Bioactivity Profiling of Marine Species. Mar. Drugs 2019, 17, 148.

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