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Open AccessArticle

Cloning, Expression, and Characterization of a New Glycosaminoglycan Lyase from Microbacterium sp. H14

by Junhao Sun 1,2,3, Xu Han 1,2,3, Guanrui Song 1,2,3, Qianhong Gong 1,2,3,* and Wengong Yu 1,2,3,*
1
School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, China
3
Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, 5 Yushan Road, Qingdao 266003, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(12), 681; https://doi.org/10.3390/md17120681
Received: 8 November 2019 / Revised: 27 November 2019 / Accepted: 28 November 2019 / Published: 2 December 2019
Glycosaminoglycan (GAG) lyase is an effective tool for the structural and functional studies of glycosaminoglycans and preparation of functional oligosaccharides. A new GAG lyase from Microbacterium sp. H14 was cloned, expressed, purified, and characterized, with a molecular weight of approximately 85.9 kDa. The deduced lyase HCLaseM belonged to the polysaccharide lyase (PL) family 8. Based on the phylogenetic tree, HCLaseM could not be classified into the existing three subfamilies of this family. HCLaseM showed almost the same enzyme activity towards hyaluronan (HA), chondroitin sulfate A (CS-A), CS-B, CS-C, and CS-D, which was different from reported GAG lyases. HCLaseM exhibited the highest activities to both HA and CS-A at its optimal temperature (35 °C) and pH (pH 7.0). HCLaseM was stable in the range of pH 5.0–8.0 and temperature below 30 °C. The enzyme activity was independent of divalent metal ions and was not obviously affected by most metal ions. HCLaseM is an endo-type enzyme yielding unsaturated disaccharides as the end products. The facilitated diffusion effect of HCLaseM is dose-dependent in animal experiments. These properties make it a candidate for further basic research and application. View Full-Text
Keywords: glycosaminoglycan lyase; oligosaccharide; characterization glycosaminoglycan lyase; oligosaccharide; characterization
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Sun, J.; Han, X.; Song, G.; Gong, Q.; Yu, W. Cloning, Expression, and Characterization of a New Glycosaminoglycan Lyase from Microbacterium sp. H14. Mar. Drugs 2019, 17, 681.

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