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Open AccessArticle

Nutraceutical Extract from Dulse (Palmaria palmata L.) Inhibits Primary Human Neutrophil Activation

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Cell Biology Unit, Instituto de la Grasa, CSIC. Ctra. de Utrera Km. 1, 41013 Seville, Spain
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Department of Food & Health, Instituto de la Grasa, CSIC. Ctra. de Utrera Km. 1, 41013 Seville, Spain
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Department of Cell Biology. Faculty of Biology. Universidad de Sevilla. Av. Reina Mercedes s/n, 41012 Seville, Spain
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Department of Medical Biochemistry, Molecular Biology, and Immunology. School of Medicine, Universidad de Sevilla, Av. Dr. Fedriani 3, 41071 Seville, Spain
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Department of Pharmacology, Pediatrics, and Radiology. School of Medicine, Universidad de Sevilla, Av. Dr. Fedriani 3, 41071 Seville, Spain
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(11), 610; https://doi.org/10.3390/md17110610
Received: 23 August 2019 / Revised: 28 September 2019 / Accepted: 16 October 2019 / Published: 25 October 2019
(This article belongs to the Special Issue Marine Nutraceuticals and Functional Foods)
Palmaria palmata L. (Palmariaceae), commonly known as “dulse”, is a red alga that grows on the northern coasts of the Atlantic and Pacific oceans, and is widely used as source of fiber and protein. Dulse is reported to contain anti-inflammatory and antioxidant compounds, albeit no study has investigated these effects in primary human neutrophils. Implication strategies to diminish neutrophil activation have the potential to prevent pathological states. We evaluated the ability of a phenolic dulse extract (DULEXT) to modulate the lipopolysaccharide (LPS)-mediated activation of primary human neutrophils. Intracellular reactive oxygen species (ROS) were measured by fluorescence analysis and nitric oxide (NO) production using the Griess reaction. Inflammatory enzymes and cytokines were detected by ELISA and RT-qPCR. The results show that DULEXT diminished the neutrophil activation related to the down-regulation of TLR4 mRNA expression, deceased gene expression and the LPS-induced release of the chemoattractant mediator IL-8 and the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α. ROS, NO, and myeloperoxidase (MPO) were also depressed. The data indicated that DULEXT has the potential to disrupt the activation of human primary neutrophils and the derived inflammatory and prooxidant conditions, and suggest a new role for Palmaria palmata L. in the regulation of the pathogenesis of health disorders in which neutrophils play a key role, including atherosclerosis. View Full-Text
Keywords: Palmaria palmata; dulse; algae; neutrophils; inflammation; nutraceutic Palmaria palmata; dulse; algae; neutrophils; inflammation; nutraceutic
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Millan-Linares, M.C.; Martin, M.E.; Rodriguez, N.M.; Toscano, R.; Claro, C.; Bermudez, B.; Pedroche, J.; Millan, F.; Montserrat-de la Paz, S. Nutraceutical Extract from Dulse (Palmaria palmata L.) Inhibits Primary Human Neutrophil Activation. Mar. Drugs 2019, 17, 610.

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