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Mar. Drugs 2018, 16(3), 83; https://doi.org/10.3390/md16030083

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

1
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan
2
Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam
3
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam
4
Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan
*
Author to whom correspondence should be addressed.
Received: 10 February 2018 / Revised: 26 February 2018 / Accepted: 6 March 2018 / Published: 8 March 2018
(This article belongs to the Special Issue Bioconversion of Marine Resources)
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Abstract

Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus. Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0.759 U/mL) was compared to that of fermented shrimp heads (0.397 U/mL), demineralized shrimp shells (0.201 U/mL) and demineralized crab shells (0.216 U/mL). A squid pen concentration of 0.5% was suitable for chitosanase, protease and αGI production via Paenibacillus sp. TKU042. Multi-purification, including ethanol precipitation and column chromatography of Macro-Prep High S as well as Macro-Prep DEAE (diethylaminoethyl), led to the isolation of Paenibacillus sp. TKU042 chitosanase and protease with molecular weights of 70 and 35 kDa, respectively. For comparison, 16 chitinolytic bacteria, including strains of Paenibacillus, were investigated for the production of chitinase, exochitinase, chitosanase, protease and αGI using two kinds of chitinous sources. View Full-Text
Keywords: chitin; chitosan; squid pens; chitosanase; protease; α-glucosidase inhibitors; Paenibacillus chitin; chitosan; squid pens; chitosanase; protease; α-glucosidase inhibitors; Paenibacillus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Doan, C.T.; Tran, T.N.; Nguyen, V.B.; Nguyen, A.D.; Wang, S.-L. Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042. Mar. Drugs 2018, 16, 83.

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