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Mar. Drugs 2017, 15(4), 94;

Outdoor Cultivation of Marine Diatoms for Year-Round Production of Biofuels

Biotechnology Laboratory, Electric Power Development Co., Ltd., 1, Yanagisaki -machi, Wakamatsu-ku, Kitakyusyu 808-0111, Japan
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
Authors to whom correspondence should be addressed.
Academic Editors: Allen Place, Rosemary Jagus, Joy Watts and Keith B. Glaser
Received: 31 January 2017 / Revised: 15 March 2017 / Accepted: 22 March 2017 / Published: 25 March 2017
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology II 2016)
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Biofuel production using microalgae is believed to have the advantage of continuous year-round production over crop plants, which have strong seasonality. However, actual year-round production of microalgal lipids using outdoor mass cultivation has rarely been demonstrated. In our previous study, it was demonstrated that the oleaginous diatom, Fistulifera solaris, was culturable in outdoor bioreactors from spring to autumn, whereas biomass and lipid production in winter failed because F. solaris did not grow below 15 °C. Therefore, another candidate strain that is culturable in winter is required. In this study, a cold-tolerant diatom, Mayamaea sp. JPCC CTDA0820, was selected as a promising candidate for biofuel production in winter. Laboratory-scale characterization revealed that this diatom was culturable at temperatures as low as 10 °C. Subsequently, F. solaris (April–October) and Mayamaea sp. JPCC CTDA0820 (November–March) were cultured in outdoor open-pond bioreactors, wherein year-round production of diatom lipids was successfully demonstrated. The maximal values of areal productivities of biomass and lipids reached to 9.79 and 1.80 g/(m2 day) for F. solaris, and 8.62 and 0.92 g/(m2 day) for Mayamaea sp. JPCC CTDA0820, respectively. With the combined use of these two diatom species, stable year-round production of microalgal lipids became possible. View Full-Text
Keywords: biofuel; diatoms; Mayamaea sp. JPCC CTDA0820; F. solaris; cold-tolerant microalgae; outdoor cultivation; open-pond bioreactor biofuel; diatoms; Mayamaea sp. JPCC CTDA0820; F. solaris; cold-tolerant microalgae; outdoor cultivation; open-pond bioreactor

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Matsumoto, M.; Nojima, D.; Nonoyama, T.; Ikeda, K.; Maeda, Y.; Yoshino, T.; Tanaka, T. Outdoor Cultivation of Marine Diatoms for Year-Round Production of Biofuels. Mar. Drugs 2017, 15, 94.

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