Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products
Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Long
Mar. Drugs 2016, 14(4), 78; https://doi.org/10.3390/md14040078
Received: 25 February 2016
/
Revised: 1 April 2016
/
Accepted: 6 April 2016
/
Published: 13 April 2016
Like many fields of the biosciences, actinomycete natural products research has been revolutionised by next-generation DNA sequencing (NGS). Hundreds of new genome sequences from actinobacteria are made public every year, many of them as a result of projects aimed at identifying new natural products and their biosynthetic pathways through genome mining. Advances in these technologies in the last five years have meant not only a reduction in the cost of whole genome sequencing, but also a substantial increase in the quality of the data, having moved from obtaining a draft genome sequence comprised of several hundred short contigs, sometimes of doubtful reliability, to the possibility of obtaining an almost complete and accurate chromosome sequence in a single contig, allowing a detailed study of gene clusters and the design of strategies for refactoring and full gene cluster synthesis. The impact that these technologies are having in the discovery and study of natural products from actinobacteria, including those from the marine environment, is only starting to be realised. In this review we provide a historical perspective of the field, analyse the strengths and limitations of the most relevant technologies, and share the insights acquired during our genome mining projects.
View Full-Text
Keywords:
actinomycetes; Streptomyces; genome mining; PacBio; Illumina; next generation sequencing
▼
Show Figures
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Supplementary File 1:
Supplementary (PDF, 362 KiB)
MDPI and ACS Style
Gomez-Escribano, J.P.; Alt, S.; Bibb, M.J. Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products. Mar. Drugs 2016, 14, 78. https://doi.org/10.3390/md14040078
AMA Style
Gomez-Escribano JP, Alt S, Bibb MJ. Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products. Marine Drugs. 2016; 14(4):78. https://doi.org/10.3390/md14040078
Chicago/Turabian StyleGomez-Escribano, Juan Pablo, Silke Alt, and Mervyn J. Bibb. 2016. "Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products" Marine Drugs 14, no. 4: 78. https://doi.org/10.3390/md14040078
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
