A Pimarane Diterpene and Cytotoxic Angucyclines from a Marine-Derived Micromonospora sp. in Vietnam’s East Sea
by
Michael W. Mullowney 1, Eoghainín Ó hAinmhire 1,2, Urszula Tanouye 1, Joanna E. Burdette 1,2, Van Cuong Pham 3 and Brian T. Murphy 1,2,*
Michael W. Mullowney 1, Eoghainín Ó hAinmhire 1,2, Urszula Tanouye 1, Joanna E. Burdette 1,2, Van Cuong Pham 3 and Brian T. Murphy 1,2,*
1
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street (MC 781), Room 539, Chicago, IL 60612-7231, USA
2
Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Molecular Biology Research Building, 900 South Ashland Avenue (MC 870), Room 3150, Chicago, IL 60607-7173, USA
3
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoàng Quốc Việt, Cầu Giấy, Hà Nội 10000, Vietnam
*
Author to whom correspondence should be addressed.
Academic Editor: Anake Kijjoa
Mar. Drugs 2015, 13(9), 5815-5827; https://doi.org/10.3390/md13095815
Received: 4 August 2015 / Revised: 2 September 2015 / Accepted: 8 September 2015 / Published: 15 September 2015
A screening of our actinomycete fraction library against the NCI-60 SKOV3 human tumor cell line led to the isolation of isopimara-2-one-3-ol-8,15-diene (1), lagumycin B (2), dehydrorabelomycin (3), phenanthroviridone (4), and WS-5995 A (5). These secondary metabolites were produced by a Micromonospora sp. isolated from sediment collected off the Cát Bà peninsula in the East Sea of Vietnam. Compound 1 is a novel Δ8,9-pimarane diterpene, representing one of approximately 20 actinomycete-produced diterpenes reported to date, while compound 2 is an angucycline antibiotic that has yet to receive formal characterization. The structures of 1 and 2 were elucidated by combined NMR and MS analysis and the absolute configuration of 1 was assigned by analysis of NOESY NMR and CD spectroscopic data. Compounds 2–5 exhibited varying degrees of cytotoxicity against a panel of cancerous and non-cancerous cell lines. Overall, this study highlights our collaborative efforts to discover novel biologically active molecules from the large, underexplored, and biodiversity-rich waters of Vietnam’s East Sea.
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Keywords:
actinomycete; marine; Micromonospora; ovarian cancer; Vietnam; diterpene
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MDPI and ACS Style
Mullowney, M.W.; Ó hAinmhire, E.; Tanouye, U.; Burdette, J.E.; Pham, V.C.; Murphy, B.T. A Pimarane Diterpene and Cytotoxic Angucyclines from a Marine-Derived Micromonospora sp. in Vietnam’s East Sea. Mar. Drugs 2015, 13, 5815-5827.
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