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Laminarin from Irish Brown Seaweeds Ascophyllum nodosum and Laminaria hyperborea: Ultrasound Assisted Extraction, Characterization and Bioactivity
by
Shekhar U. Kadam 1,
Colm P. O'Donnell 1,
Dilip K. Rai 2
,
Mohammad B. Hossain 2,
Catherine M. Burgess 3,
Des Walsh 3 and
Brijesh K. Tiwari 2,*
Shekhar U. Kadam 1,
Colm P. O'Donnell 1,
Dilip K. Rai 2,
Mohammad B. Hossain 2,
Catherine M. Burgess 3,
Des Walsh 3 and
Brijesh K. Tiwari 2,*
1
School of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin 4, Ireland
2
Food Biosciences, Teagasc Food Research Centre, Dublin 15, Ireland
3
Food Safety, Teagasc Food Research Centre, Dublin 15, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Paola Laurienzo
Mar. Drugs 2015, 13(7), 4270-4280; https://doi.org/10.3390/md13074270
Received: 14 April 2015 / Revised: 30 June 2015 / Accepted: 1 July 2015 / Published: 10 July 2015
(This article belongs to the Collection Marine Polysaccharides)
Ultrasound assisted extraction (UAE), purification, characterization and antioxidant activity of laminarin from Irish brown seaweeds Ascophyllum nodosum and Laminarina hyperborea were investigated. UAE was carried out using 60% ultrasonic power amplitude and 0.1 M hydrochloric acid for 15 min. Separately, solid-liquid extraction was carried in an orbital shaker using 0.1 M hydrochloric acid at 70 °C for 2.5 h. UAE with hydrochloric acid resulted in the highest concentration of laminarin, 5.82% and 6.24% on dry weight basis from A. nodosum and L. hyperborea, respectively. Purification of all extracts was carried out using molecular weight cut off dialysis at 10 kDa. Characterization of the laminarin fraction was carried out using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Antioxidant activity of A. nodosum and L. hyperborea extracts had 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition levels of 93.23% and 87.57%, respectively. Moreover, these extracts have shown inihibition of bacterial growth of Staphylcoccus aureus, Listeria monocytogenes, Escherichia coli and Salmonella typhimurium.
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Keywords:
laminarin; antioxidant; antimicrobial; bioactive; Laminaria hyperborea; Ascophyllum nodosum
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MDPI and ACS Style
Kadam, S.U.; O'Donnell, C.P.; Rai, D.K.; Hossain, M.B.; Burgess, C.M.; Walsh, D.; Tiwari, B.K. Laminarin from Irish Brown Seaweeds Ascophyllum nodosum and Laminaria hyperborea: Ultrasound Assisted Extraction, Characterization and Bioactivity. Mar. Drugs 2015, 13, 4270-4280.
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