Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation of Fucoidan and Oligosaccharides
Sample | Fuc (%) | U A (%) | SO4 (%) | Monosaccharides (molar ratio) | Mw | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Fuc | Gal | Man | Glc A | Rha | Xyl | Glc | |||||
F0.5 | 13.77 | 20.34 | 29.07 | 1 | 0.98 | 0.80 | 0.95 | 0.12 | 0.30 | 0.40 | 5954 |
F1 | 54.84 | 7.3 | 32.26 | 1 | 0.36 | 0.13 | 0.10 | 0.02 | 0.04 | 0.10 | 8436 |
F2 | 35.04 | 0.71 | 53.40 | 1 | 0.07 | 0.03 | 0.01 | 0 | 0 | 0.02 | 12586 |
YF | - | - | - | 1 | 0.43 | 0.32 | 0.28 | 0.41 | 0 | 0.26 | - |
YD-1 | - | - | - | 0 | 1.10 | 11.14 | 12.59 | 0.17 | 0 | 1.08 | - |
YD-2 | - | - | - | 1 | 0.28 | 3.53 | 1.96 | 0 | 0 | 0.29 | - |
YT | - | - | - | 0 | 0 | 8.21 | 9.30 | 0 | 0 | 1.48 | - |
G5 | - | - | - | 1 | 0.11 | 0.02 | 0 | 0 | 0 | 0.07 | - |
G6 | - | - | - | 1 | 0.04 | 0 | 0 | 0 | 0 | 0.05 | - |
Samples | DP | Ions (charges) | m/z | Predicted structural compositions | |
---|---|---|---|---|---|
YF | 1 | 193.035(−1) | 193.035 | GlcA | |
2 | 355.086(−1) | 355.086 | GlcAMan | ||
2 | 369.065(−1) | 369.065 | GlcA2 | ||
2 | 339.091(−1) | 339.091 | GlcAFuc | ||
3 | 531.117(−1) | 531.117 | GlcA2Man | ||
4 | 346.081(−2) | 693.170 | GlcA2Man2 | ||
693.170(−1) | |||||
6 | 515.123(−2) | 1031.249 | GlcA3Man3 | ||
YD | YD-1 | 2 | 184.032(−2) | 369.065 | GlcA2 |
369.065(−1) | |||||
2 | 217.018(−2) | 435.030 | GlcAManSO3H | ||
3 | 265.055(−2) | 531.117 | GlcA2Man | ||
531.117(−1) | |||||
4 | 346.081(−2) | 693.170 | GlcA2Man2 | ||
693.170(−1) | |||||
4 | 386.059(−2) | 773.117 | GlcA2Man2SO3H | ||
6 | 515.123(−2) | 1031.249 | GlcA3Man3 | ||
YD-2 | 1 | 193.035(−1) | 193.035 | GlcA | |
1 | 243.017(−1) | 243.017 | FucSO3H | ||
1 | 259.012(−1) | 259.012 | GalSO3H | ||
2 | 339.231(−1) | 339.231 | GlcAFuc | ||
2 | 355.086(−1) | 355.086 | GlcAMan | ||
2 | 217.072(−2) | 435.033 | GlcAManSO3H | ||
YT | 2 | 217.072(−2) | 435.033 | GlcAManSO3H | |
3 | 257.037(−3) | 545.096 | GlcA3 | ||
3 | 305.033(−2) | 611.068 | GlcA2ManSO3H | ||
4 | 386.059(−2) | 773.118 | GlcA2Man2SO3H | ||
5 | 474.073(−2) | 949.147 | GlcA3Man2SO3H | ||
6 | 369.731(−3) | 1111.201 | GlcA3Man3SO3H | ||
555.101(−2) | |||||
G1 | 8 | 1369.333(−1) | 1369.333 | GlcA4Man4 | |
G2 | 6 | 343.081(−3) | 1031.249 | GlcA3Man3 | |
515.125(−2) | |||||
1031.249(−1) | |||||
G3 | 4 | 346.082(−2) | 693.173 | GlcA2Man2 | |
693.173(−1) | |||||
G4 | 2 | 355.086(−1) | 355.086 | GlcAMan |
2.2. Analysis of the Oligosaccharides of All Fractions by ESI-MS
2.3. Analysis of the Structural Features of the Fractions by ESI-CID-MS/MS
3. Experimental Section
3.1. Materials
3.2. Preparation and Purification of Fucoidans
3.3. Preparation and Purification of Oligosaccharides from F0.5
3.4. Composition Analysis
3.5. MS Analysis of Oligosaccharides
3.6. Condition of HPLC
4. Conclusions
Supplementary Files
Acknowledgments
References
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Jin, W.; Wang, J.; Ren, S.; Song, N.; Zhang, Q. Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS). Mar. Drugs 2012, 10, 2138-2152. https://doi.org/10.3390/md10102138
Jin W, Wang J, Ren S, Song N, Zhang Q. Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS). Marine Drugs. 2012; 10(10):2138-2152. https://doi.org/10.3390/md10102138
Chicago/Turabian StyleJin, Weihua, Jing Wang, Sumei Ren, Ni Song, and Quanbin Zhang. 2012. "Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)" Marine Drugs 10, no. 10: 2138-2152. https://doi.org/10.3390/md10102138
APA StyleJin, W., Wang, J., Ren, S., Song, N., & Zhang, Q. (2012). Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS). Marine Drugs, 10(10), 2138-2152. https://doi.org/10.3390/md10102138