A Benzimidazole-Based N-Heterocyclic Carbene Derivative Exhibits Potent Antiproliferative and Apoptotic Effects against Colorectal Cancer
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. IMBZC Synthesis and Preparation
2.4. MTT Cytotoxicity Assay
2.5. Scratch Wound-Healing Assay
2.6. Clonogenic Assay
2.7. Real-Time Reverse Transcription–Quantitative Polymerase Chain Reaction (RT-qPCR)
2.8. Western Blot Technology
2.9. Statistical Analysis
3. Results
3.1. IMBZC Compound Inhibits CRC Cell Viability in a Dose-Dependent Manner
3.2. IMBZC Compound Decreases CRC Cell Migration and Inhibits Colony Formation
3.3. IMBZC Downregulates the Expression Levels of Anti-Apoptotic Bcl-2 and Bcl-xL While Upregulating Pro-Apoptotic Bax and p53 in a Dose-Dependent Manner
3.4. IMBZC Enhances the Cytotoxic Effects of Conventional CRC Drugs 5-FU, IRI, and OXA
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Target Gene | Primer Sequence 5′→3′ |
---|---|
GAPDH | GTCTCCTCTGACTTCAACAGCG (forward) |
ACCACCCTGTTGCTGTAGCCAA (reverse) | |
BAX | CCCGAGAGGTCTTTTTCCGAG (forward) |
CCAGCCCATGATGGTTCTGAT (reverse) | |
BCL2 | TCCGCATCAGGAAGGCTAGA (forward) |
AGGACCAGGCCTCCAAGCT (reverse) | |
BCL-xL | AGTTCCCTTGGCCTCAGAAT (forward) |
TCCTTTCTGGGGAAGAGGTT (reverse) | |
TP53 | CCTCAGCATCTTATCCGAGTGG (forward) |
TGAGGCTCACGTCCATCTCGTC (reverse) |
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Al-Nasser, S.; Abdulla, M.H.; Alhassan, N.; Vaali-Mohammed, M.-A.; Al-Omar, S.; Hamdi, N.; Elnakady, Y.; Matou-Nasri, S.; Mansour, L. A Benzimidazole-Based N-Heterocyclic Carbene Derivative Exhibits Potent Antiproliferative and Apoptotic Effects against Colorectal Cancer. Medicina 2024, 60, 1379. https://doi.org/10.3390/medicina60091379
Al-Nasser S, Abdulla MH, Alhassan N, Vaali-Mohammed M-A, Al-Omar S, Hamdi N, Elnakady Y, Matou-Nasri S, Mansour L. A Benzimidazole-Based N-Heterocyclic Carbene Derivative Exhibits Potent Antiproliferative and Apoptotic Effects against Colorectal Cancer. Medicina. 2024; 60(9):1379. https://doi.org/10.3390/medicina60091379
Chicago/Turabian StyleAl-Nasser, Sarah, Maha Hamadien Abdulla, Noura Alhassan, Mansoor-Ali Vaali-Mohammed, Suliman Al-Omar, Naceur Hamdi, Yasser Elnakady, Sabine Matou-Nasri, and Lamjed Mansour. 2024. "A Benzimidazole-Based N-Heterocyclic Carbene Derivative Exhibits Potent Antiproliferative and Apoptotic Effects against Colorectal Cancer" Medicina 60, no. 9: 1379. https://doi.org/10.3390/medicina60091379
APA StyleAl-Nasser, S., Abdulla, M. H., Alhassan, N., Vaali-Mohammed, M.-A., Al-Omar, S., Hamdi, N., Elnakady, Y., Matou-Nasri, S., & Mansour, L. (2024). A Benzimidazole-Based N-Heterocyclic Carbene Derivative Exhibits Potent Antiproliferative and Apoptotic Effects against Colorectal Cancer. Medicina, 60(9), 1379. https://doi.org/10.3390/medicina60091379