Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Population and Sample Collection
2.2. Semen Analysis
- Fertile Men:
- Defined by having a sperm concentration of ≥20 × 106 cells/mL and sperm motility of >40%.
- 2.
- Sub-fertile Men:
- Oligospermia: Men with a low sperm count of <15 million.
- Asthenospermia: Men with sperm motility of <40%.
- Oligoasthenospermia: Men with both low sperm count of <15 million and low motility of <40%.
2.3. Measurement of VIP and PACAP Levels in Seminal Plasma
2.4. Statistical Analysis
3. Results
3.1. Subjects’ Demographic Characteristics, Semen Criteria, and Protein Levels
3.2. Comparison between Sub-Fertile Patients’ Groups and Fertile Males’ Group
3.3. Associations between Seminal Levels of VIP and PACAP and Seminal Parameters of Sub-Fertile Patients
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fertile Males (n = 81) | Sub-Fertile Patients (n = 82) | p-Value | |
---|---|---|---|
Age | 31.63 ± 0.54 | 31.90 ± 0.55 | 0.724 |
Height | 175.95 ± 0.60 | 177.49 ± 0.54 | 0.058 |
Weight | 90.57 ± 1.25 | 89.87 ± 1.26 | 0.693 |
BMI | 29.17 ± 0.29 | 28.46 ± 0.31 | 0.094 |
Liquefaction time (min) | 29.14 ± 1.11 | 32.19 ± 1.09 | 0.052 |
Total sperm count (106/mL) | 56.56 ± 2.83 | 11.20 ± 2.0 | <0.001 |
Semen volume (mL) | 3.83 ± 0.17 | 3.15 ± 0.38 | 0.108 |
Sperm concentration (106/mL) | 17.38 ± 1.22 | 4.46 ± 1.14 | <0.001 |
Total motility (%) | 66.96 ± 1.07 | 23.98 ± 2.81 | <0.001 |
Normal morphology (%) | 32.01 ± 3.13 | 31.00 ± 3.33 | 0.825 |
Viscosity | 0.28 ± 0.08 | 0.44 ± 0.09 | 0.191 |
Vitality, n (%) | <0.001 * | ||
| 79 (97.5) | 15 (18.3) | |
| 2 (2.5) | 56 (68.3) | |
| 0 (0.0) | 11 (13.4) | |
PACAP | 14.87 ± 1.6 | 22.15 ± 2.30 | 0.011 |
VIP | 62.18 ± 3.76 | 90.47 ± 29.74 | 0.350 |
F (n = 81) | O (n = 19) | A (n = 12) | OA (n = 38) | Z (n = 13) | p-Value | |
---|---|---|---|---|---|---|
Age | 31.63 ± 0.54 | 30.42 ± 0.91 | 31.92 ± 1.25 | 31.63 ± 0.87 | 34.85 ± 1.40 | 0.154 |
Liquefaction time (min) | 29.14 ± 1.11 | 31.32 ± 2.41 | 29.58 ± 2.34 | 34.34 ± 1.52 | 29.09 ± 3.36 | 0.108 |
Total sperm count (106/mL) | 56.56 ± 2.83 | 10.98 ± 4.21 | 37.75 ± 7.16 | 6.16 ± 0.87 | 0 | <0.001 |
Semen volume (mL) | 3.83 ± 0.17 | 4.85 ± 1.44 | 3.59 ± 0.58 | 2.62 ± 0.22 | 1.84 ± 0.60 | 0.004 |
Sperm concentration (106/mL) | 17.38 ± 1.22 | 6.26 ± 4.38 | 12.20 ± 2.41 | 2.64 ± 0.33 | 0 | <0.001 |
Total motility (%) | 66.96 ± 1.07 | 56.8 ± 2.41 | 25.14 ± 3.88 | 16.59 ± 2.62 | 0 | <0.001 |
Normal morphology (%) | 32.01 ± 3.13 | 39.11 ± 7.26 | 21.50 ± 7.37 | 38.92 ± 4.57 | 0 | 0.001 |
Viscosity | 0.28 ± 0.08 | 0.26 ± 0.13 | 0.83 ± 0.27 | 0.39 ± 0.13 | 0.44 ± 0.24 | 0.168 |
Vitality, n (%) | <0.001 | |||||
| 79 (97.5) | 11 (57.9) | 0 (0.0) | 2 (5.3) | 2 (15.4) | |
| 2 (2.5) | 8 (42.1) | 12 (100) | 36 (94.7) | 11 (84.6) | |
PACAP | 14.87 ± 1.6 | 22.68 ± 3.50 | 11.62 ± 2.01 | 28.18 ± 4.23 | 13.48 ± 2.84 | 0.001 * |
VIP | 62.18 ± 3.76 | 61.54 ± 9.03 | 69.07 ± 13.52 | 126.46 ± 63.77 | 47.34 ± 8.78 | 0.491 |
Sub-Fertile Patients | ||
---|---|---|
PACAP | VIP | |
Age | ||
r | −0.142 | −0.082 |
p-value | 0.204 | 0.465 |
Liquefaction time (min) | ||
r | 0.282 | 0.118 |
p-value | 0.011 | 0.295 |
Total sperm count (106/mL) | ||
r | −0.189 | −0.067 |
p-value | 0.094 | 0.554 |
Semen volume (mL) | ||
r | −0.066 | −0.047 |
p-value | 0.558 | 0.673 |
Sperm concentration (106/mL) | ||
r | −0.135 | −0.041 |
p-value | 0.226 | 0.715 |
Total motility (%) | ||
r | −0.063 | −0.122 |
p-value | 0.618 | 0.331 |
Normal morphology (%) | ||
r | 0.392 | 0.130 |
p-value | <0.001 | 0.251 |
Viscosity | ||
r | −0.168 | −0.065 |
p-value | 0.142 | 0.570 |
PACAP | ||
PCC | -- | 0.626 |
p-value | -- | <0.001 |
VIP | ||
r | 0.626 | -- |
p-value | <0.001 | -- |
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Bdeir, R.; Al-Keilani, M.S.; Khamaiseh, K. Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility. Medicina 2024, 60, 652. https://doi.org/10.3390/medicina60040652
Bdeir R, Al-Keilani MS, Khamaiseh K. Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility. Medicina. 2024; 60(4):652. https://doi.org/10.3390/medicina60040652
Chicago/Turabian StyleBdeir, Roba, Maha S. Al-Keilani, and Khaldoun Khamaiseh. 2024. "Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility" Medicina 60, no. 4: 652. https://doi.org/10.3390/medicina60040652
APA StyleBdeir, R., Al-Keilani, M. S., & Khamaiseh, K. (2024). Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility. Medicina, 60(4), 652. https://doi.org/10.3390/medicina60040652