Whey Peptide Alleviates Muscle Atrophy by Strongly Regulating Myocyte Differentiation in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Test Material
2.2. Reagents
2.3. Experimental Animals
2.4. Muscle Atrophy Induction and Treatment
2.5. Grip Strength Measurement
2.6. Histological Analysis
2.7. Blood Analysis
2.8. Micro-Computer Tomography (CT) Measurement
2.9. Tissue Western Blotting
2.10. Statistical Analysis
3. Results
3.1. Changes in Body Weight and Food Intake
3.2. Muscle Changes
3.3. Grip Strength Measurement
3.4. Analysis of Markers Related to Muscle Damage and Kidney Function
3.5. Analysis of Inflammatory Cytokines in Serum
3.6. Analysis of Proteins Related to Muscle Synthesis
3.7. Analysis of Proteins Related to Myolysis
3.8. Expression of Proteins Related to Myocyte Differentiation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Body Weight (g) | Food Intake (g) | |||
---|---|---|---|---|
Initial | Final | Gain | ||
Normal | 19.17 ± 0.82 | 27.23 ± 2.23 | 8.06 ± 2.01 | 2.75 ± 0.39 |
DEX | 19.03 ± 0.86 | 23.63 ± 1.20 ### | 4.60 ± 0.70 ### | 2.74 ± 0.60 |
Oxy | 19.26 ± 0.91 | 24.63 ± 1.52 | 5.37 ± 1.46 | 2.79 ± 0.32 |
WPCH | 19.11 ± 0.78 | 23.98 ± 1.80 | 4.87 ± 1.57 | 2.84 ± 0.47 |
WPL | 19.30 ± 0.49 | 23.75 ± 0.95 | 4.45 ± 1.15 | 2.66 ± 0.51 |
WPH | 19.33 ± 0.57 | 24.20 ± 1.71 | 4.87 ± 1.86 | 2.88 ± 0.55 |
Muscle Damage (IU/L) | Kidney Function (mg/dL) | |||
---|---|---|---|---|
AST | ALT | BUN | Creatinine | |
Normal | 12.29 ± 0.63 | 4.31 ± 0.36 | 17.86 ± 0.52 | 0.19 ± 0.01 |
DEX | 29.31 ± 3.97 ### | 7.31 ± 1.36 # | 20.68 ± 0.27 ### | 0.62 ± 0.04 ### |
Oxy | 28.83 ± 2.40 | 6.45 ± 0.54 | 19.08 ± 0.36 * | 0.58 ± 0.04 |
WPCH | 23.62 ± 3.25 | 5.08 ± 0.40 * | 17.25 ± 0.37 *** | 0.52 ± 0.02 * |
WPL | 21.63 ± 2.09 | 5.29 ± 0.96 | 15.16 ± 0.73 *** | 0.52 ± 0.02 * |
WPH | 20.70 ± 3.08 * | 4.96 ± 0.48 * | 17.64 ± 0.10 *** | 0.51 ± 0.03 * |
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Lee, J.A.; Shin, M.-R.; Kim, M.; Kim, H.-Y.; Choi, H.-Y.; Seo, Y.; Choi, H.; Roh, S.-S. Whey Peptide Alleviates Muscle Atrophy by Strongly Regulating Myocyte Differentiation in Mice. Medicina 2024, 60, 433. https://doi.org/10.3390/medicina60030433
Lee JA, Shin M-R, Kim M, Kim H-Y, Choi H-Y, Seo Y, Choi H, Roh S-S. Whey Peptide Alleviates Muscle Atrophy by Strongly Regulating Myocyte Differentiation in Mice. Medicina. 2024; 60(3):433. https://doi.org/10.3390/medicina60030433
Chicago/Turabian StyleLee, Jin A, Mi-Rae Shin, Minju Kim, Hwa-Young Kim, Hwang-Yong Choi, Yoojin Seo, Hakjoo Choi, and Seong-Soo Roh. 2024. "Whey Peptide Alleviates Muscle Atrophy by Strongly Regulating Myocyte Differentiation in Mice" Medicina 60, no. 3: 433. https://doi.org/10.3390/medicina60030433