Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line
Abstract
Materials and methods: DOX-resistant CX-1 cell sublines were acquired by stepwise increment of DOX concentrations in cell growth media. Global gene expression profiling was performed using human gene expression microarrays. The expression levels of individual genes were assessed by means of quantitative PCR (qPCR), while the DNA methylation pattern of several selected genes was determined by methylation-specific PCR.
Results: Four DOX-resistant CX-1 sublines were established as a valuable tool for cell chemoresistance studies. Altered expression of the EMT, cell adhesion and motility, and chemoresistance-related genes was observed in DOX-resistant cells by genome-wide gene expression analysis. Besides, early and significant upregulation of the key EMT genes ZEB1 (5.8×; P < 0.001) and CDH2 (6.2×; P = 0.044) was identified by qPCR, with subsequent activation of drug transporter gene ABCC1 (3.3×; P = 0.007) and cell stemness gene NANOG (2.4×; P = 0.008). Downregulation of TET1 (2.1×; P = 0.041) and changes in the methylation status of the p16 gene were also involved in the acquisition of cell resistance to DOX.
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Kubiliūtė, R.; Šulskytė, I.; Daniūnaitė, K.; Daugelavičius, R.; Jarmalaitė, S. Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line. Medicina 2016, 52, 298-306. https://doi.org/10.1016/j.medici.2016.09.003
Kubiliūtė R, Šulskytė I, Daniūnaitė K, Daugelavičius R, Jarmalaitė S. Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line. Medicina. 2016; 52(5):298-306. https://doi.org/10.1016/j.medici.2016.09.003
Chicago/Turabian StyleKubiliūtė, Raimonda, Indrė Šulskytė, Kristina Daniūnaitė, Rimantas Daugelavičius, and Sonata Jarmalaitė. 2016. "Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line" Medicina 52, no. 5: 298-306. https://doi.org/10.1016/j.medici.2016.09.003
APA StyleKubiliūtė, R., Šulskytė, I., Daniūnaitė, K., Daugelavičius, R., & Jarmalaitė, S. (2016). Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line. Medicina, 52(5), 298-306. https://doi.org/10.1016/j.medici.2016.09.003