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Volume 48
Issue 5
10.3390/cimb48050521
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Genome-Wide Analysis of bZIP Transcription Factors and Expression Patterns in Response to Shading Treatment in Taxus yunnanensis

by
Jiangtao Fan
1,2,†,
Pengpeng Gong
1,2,†,
Yujia Liu
1,2,
Mengke Dou
1,2,
Qing Li
1,2,
Qiuhong Hu
1,2,
Yong Wang
1,2,
Gang Wang
1,2,* and
Xiong Huang
1,2,*
1
National Forestry and Grassland Southwest Engineering Technology Research Centre of Taxus, Sichuan Agricultural University, Dujiangyan 611800, China
2
Forest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 610000, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Curr. Issues Mol. Biol. 2026, 48(5), 521; https://doi.org/10.3390/cimb48050521 (registering DOI)
Submission received: 17 April 2026 / Revised: 10 May 2026 / Accepted: 15 May 2026 / Published: 17 May 2026
(This article belongs to the Special Issue Functional Genomics and Comparative Genomics Analysis in Plants, 4th Edition)
Versions Notes

Abstract

Basic leucine zipper (bZIP) transcription factors are widely involved in plant growth, development, environmental adaptation, and secondary metabolism. However, the bZIP gene family in Taxus yunnanensis has not been systematically characterized, and its potential involvement in shading-responsive regulation of paclitaxel biosynthesis remains unclear. In this study, a genome-wide analysis was performed to identify and characterize the bZIP family in T. yunnanensis. Phylogenetic analysis, conserved motif and domain identification, promoter cis-element analysis, chromosomal localization, and expression profiling were conducted to investigate their structural features and regulatory potential. A total of 18 TyubZIP genes were identified and classified into 10 subfamilies. These genes exhibited variation in physicochemical properties but showed conserved structural features and nuclear localization. Promoter analysis revealed abundant light-responsive, hormone-related, and stress-related cis-elements. Expression profiling indicated tissue-specific expression patterns and diverse responses to shading treatment. WGCNA further identified candidate TyubZIP genes potentially associated with paclitaxel biosynthesis. Among them, TyuHY5 was selected for functional analysis. Subcellular localization and transcriptional assays demonstrated that TyuHY5 can bind to the promoter of TyuDBTNBT and positively regulate its activity. These findings provide the first genome-wide characterization of the bZIP family in T. yunnanensis and identify TyuHY5 as a shading-responsive candidate regulator of paclitaxel biosynthesis, providing insights that may inform the genetic improvement and cultivation strategies of Taxus for enhanced paclitaxel production.
Keywords: Taxus yunnanensis; shading response; bZIP family genes; taxol biosynthesis Taxus yunnanensis; shading response; bZIP family genes; taxol biosynthesis

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MDPI and ACS Style

Fan, J.; Gong, P.; Liu, Y.; Dou, M.; Li, Q.; Hu, Q.; Wang, Y.; Wang, G.; Huang, X. Genome-Wide Analysis of bZIP Transcription Factors and Expression Patterns in Response to Shading Treatment in Taxus yunnanensis. Curr. Issues Mol. Biol. 2026, 48, 521. https://doi.org/10.3390/cimb48050521

AMA Style

Fan J, Gong P, Liu Y, Dou M, Li Q, Hu Q, Wang Y, Wang G, Huang X. Genome-Wide Analysis of bZIP Transcription Factors and Expression Patterns in Response to Shading Treatment in Taxus yunnanensis. Current Issues in Molecular Biology. 2026; 48(5):521. https://doi.org/10.3390/cimb48050521

Chicago/Turabian Style

Fan, Jiangtao, Pengpeng Gong, Yujia Liu, Mengke Dou, Qing Li, Qiuhong Hu, Yong Wang, Gang Wang, and Xiong Huang. 2026. "Genome-Wide Analysis of bZIP Transcription Factors and Expression Patterns in Response to Shading Treatment in Taxus yunnanensis" Current Issues in Molecular Biology 48, no. 5: 521. https://doi.org/10.3390/cimb48050521

APA Style

Fan, J., Gong, P., Liu, Y., Dou, M., Li, Q., Hu, Q., Wang, Y., Wang, G., & Huang, X. (2026). Genome-Wide Analysis of bZIP Transcription Factors and Expression Patterns in Response to Shading Treatment in Taxus yunnanensis. Current Issues in Molecular Biology, 48(5), 521. https://doi.org/10.3390/cimb48050521

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