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Volume 47
Issue 12
10.3390/cimb47120995
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Application of Mulberry (Morus nigra) Anthocyanin Extract Combined with Carboxymethyl Chitosan for Postharvest Preservation of Strawberry (Fragaria x ananassa)

by
Baogang Zhou
1,2,†,
Haibao Tang
1,2,†,
Ran Liu
1,2,
Chengfei Huang
1,
Sarengaowa
1 and
Wenzhong Hu
1,*
1
College of Life Science, Zhuhai College of Science and Technology, Zhuhai 519041, China
2
College of Life Science, Jilin University, Changchun 130012, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Curr. Issues Mol. Biol. 2025, 47(12), 995; https://doi.org/10.3390/cimb47120995 (registering DOI)
Submission received: 3 October 2025 / Revised: 17 November 2025 / Accepted: 26 November 2025 / Published: 27 November 2025
(This article belongs to the Section Molecular Plant Sciences)
Versions Notes

Abstract

Strawberries are highly susceptible to postharvest deterioration, including softening, decay, and nutrient loss, which severely limits their shelf life and commercial value. This study developed and evaluated a natural composite coating combining mulberry anthocyanin extract from Morus nigra fruits and carboxymethyl chitosan to extend the postharvest life of strawberries. The coating was rationally designed based on complementary functions: carboxymethyl chitosan acts as a semi-permeable barrier reducing water loss and microbial infestation, while mulberry anthocyanin provides antioxidant activity to mitigate oxidative stress-induced senescence. The anthocyanin extract contained cyanidin-3-glucoside (32.9%) and cyanidin-3-rutinoside (29.7%) as major components and exhibited good radical scavenging activity. Strawberries were treated with the composite coating, carboxymethyl chitosan alone, potassium sorbate, or left untreated, and then stored at 25 °C for five days. The composite coating treatment was most effective, significantly reducing weight loss, maintaining firmness, inhibiting browning, and lowering malondialdehyde accumulation compared to other treatments. These findings demonstrate that the mulberry anthocyanin-carboxymethyl chitosan composite coating is a promising natural strategy for strawberry preservation.
Keywords: edible coating; shelf life extension; natural preservatives; fruit quality maintenance; antioxidant activity edible coating; shelf life extension; natural preservatives; fruit quality maintenance; antioxidant activity

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MDPI and ACS Style

Zhou, B.; Tang, H.; Liu, R.; Huang, C.; Sarengaowa; Hu, W. Application of Mulberry (Morus nigra) Anthocyanin Extract Combined with Carboxymethyl Chitosan for Postharvest Preservation of Strawberry (Fragaria x ananassa). Curr. Issues Mol. Biol. 2025, 47, 995. https://doi.org/10.3390/cimb47120995

AMA Style

Zhou B, Tang H, Liu R, Huang C, Sarengaowa, Hu W. Application of Mulberry (Morus nigra) Anthocyanin Extract Combined with Carboxymethyl Chitosan for Postharvest Preservation of Strawberry (Fragaria x ananassa). Current Issues in Molecular Biology. 2025; 47(12):995. https://doi.org/10.3390/cimb47120995

Chicago/Turabian Style

Zhou, Baogang, Haibao Tang, Ran Liu, Chengfei Huang, Sarengaowa, and Wenzhong Hu. 2025. "Application of Mulberry (Morus nigra) Anthocyanin Extract Combined with Carboxymethyl Chitosan for Postharvest Preservation of Strawberry (Fragaria x ananassa)" Current Issues in Molecular Biology 47, no. 12: 995. https://doi.org/10.3390/cimb47120995

APA Style

Zhou, B., Tang, H., Liu, R., Huang, C., Sarengaowa, & Hu, W. (2025). Application of Mulberry (Morus nigra) Anthocyanin Extract Combined with Carboxymethyl Chitosan for Postharvest Preservation of Strawberry (Fragaria x ananassa). Current Issues in Molecular Biology, 47(12), 995. https://doi.org/10.3390/cimb47120995

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