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Volume 46
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Article

Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB

by
Zuchang Zhong
1,2,†,
Benqiang Li
1,3,4,†,
Jie Tao
1,3,4,
Jinghua Cheng
1,3,4,
Ying Shi
1,3,4,
Pan Tang
1,3,4,
Jiajie Jiao
1,3,4 and
Huili Liu
1,2,3,4,*
1
Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
2
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
3
Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai 201106, China
4
Shanghai Engineering Research Center of Pig Breeding, Shanghai 201302, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Curr. Issues Mol. Biol. 2024, 46(9), 9821-9830; https://doi.org/10.3390/cimb46090583
Submission received: 8 July 2024 / Revised: 25 August 2024 / Accepted: 30 August 2024 / Published: 3 September 2024
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Porcine sapelovirus (PSV) is a new pathogen that negatively impacts the pig industry in China. Affected pigs experience severe diarrhea and even death. Vaccination is used to control disease outbreaks, and sensitive diagnostic methods that can distinguish infected animals from vaccinated animals (DIVA) are essential for monitoring the effectiveness of disease control programs. Tests based on the detection of the nonstructural protein (NSP) 3AB are reliable indicators of viral replication in infected and vaccinated animals. In this study, the recombinant PSV 3AB protein was expressed by a prokaryotic expression system, and an indirect ELISA method was established. Serum samples from healthy animals, immunized animals, and infected animals were evaluated. The ELISA method identified 3AB with high sensitivity (99.78%) and specificity (100.0%), and no cross-reaction was observed with serum antibodies against porcine reproductive and respiratory syndrome virus (PRRSV), infection with classical swine fever virus (CSFV), pseudorabies virus (PRV), bovine viral diarrhea virus (BVDV), porcine epidemic diarrhea virus (PEDV), or foot-and-mouth disease virus (FMDV). The ELISA method described here can effectively distinguish infected and vaccinated animals and is an important inexpensive tool for monitoring serum and controlling PSV.
Keywords: porcine sapelovirus; 3AB; indirect ELISA; infected and vaccinated animals porcine sapelovirus; 3AB; indirect ELISA; infected and vaccinated animals

Share and Cite

MDPI and ACS Style

Zhong, Z.; Li, B.; Tao, J.; Cheng, J.; Shi, Y.; Tang, P.; Jiao, J.; Liu, H. Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB. Curr. Issues Mol. Biol. 2024, 46, 9821-9830. https://doi.org/10.3390/cimb46090583

AMA Style

Zhong Z, Li B, Tao J, Cheng J, Shi Y, Tang P, Jiao J, Liu H. Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB. Current Issues in Molecular Biology. 2024; 46(9):9821-9830. https://doi.org/10.3390/cimb46090583

Chicago/Turabian Style

Zhong, Zuchang, Benqiang Li, Jie Tao, Jinghua Cheng, Ying Shi, Pan Tang, Jiajie Jiao, and Huili Liu. 2024. "Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB" Current Issues in Molecular Biology 46, no. 9: 9821-9830. https://doi.org/10.3390/cimb46090583

APA Style

Zhong, Z., Li, B., Tao, J., Cheng, J., Shi, Y., Tang, P., Jiao, J., & Liu, H. (2024). Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB. Current Issues in Molecular Biology, 46(9), 9821-9830. https://doi.org/10.3390/cimb46090583

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