Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects and Sample Preparation
2.2. RNA Isolation, cDNA Preparation, and Real-Time PCR Analysis
2.3. Protein Extraction and Western Blotting
2.4. Statistical Analysis
3. Results
3.1. tTG and Inflammatory Proteins Expressions in Duodenal Biopsy Samples of CD Patients
3.2. Apoptosis-Related Protein Expressions in Duodenal Biopsy Samples of CD Patients
3.3. HIF-1α Protein and mRNA Expressions in Duodenal Biopsy Samples of CD Patients
3.4. PRDXs mRNA Expressions in Duodenal Samples of CD Patients
3.5. PRDXs Protein Expressions in Duodenal Samples of CD Patients
4. Discussion
4.1. tTG and Inflammatory Proteins Expression in Duodenal Tissue of Pediatric CD Patients
4.2. Apoptosis-Related Caspase-3 and p53 Protein Expressions in Duodenal Tissue of Pediatric CD Patients
4.3. Hypoxia-Inducible Factor (HIF-1α) mRNA and Protein Expressions in Duodenal Tissue of Pediatric CD Patients
4.4. Peroxiredoxins mRNA and Protein Expressions in Duodenal Tissue of Pediatric CD Patients
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient No. | MARSH Classifications | Serum EMA-IgA 1 | Serum tTG 2 Levels |
---|---|---|---|
1 | Type 3a | + | >200 IU |
2 | Type 3b | +++ | >200 IU |
3 | Type 3a | +++ | >200 IU |
4 | Type 2a | +++ | >200 IU |
5 | Type 3c | +++ | >200 IU |
6 | Type 3c | + | >200 IU |
7 | Type 3c | +++ | >200 IU |
Gene | Forward Primer (5′ → 3′) | Reverse Primer (5′ → 3′) | Base Pair |
---|---|---|---|
PRDX-1 (NM_181697.3) | 5′tttggtatcagacccgaagc3′ | 5′agtgaactggaaggcctgaa3′ | 185 bp |
PRDX-2 (NM_005809.6) | 5′agatcatcgcgttcagcaac 3′ | 5′atcctcagacaagcgtctgg3′ | 182 bp |
PRDX-3 (NM_001302272.2) | 5′gtcgcagtctcagtggattc3′ | 5′aacagcacaccgtagtctcg3′ | 140 bp |
PRDX-4 (NM_006406.2) | 5′aacagctgtgatcgatggag3′ | 5′tcaagtctgtcgccaaaagc3′ | 138 bp |
PRDX-5 (NM_012094.5) | 5′caagaagggtgtgctgtttg3′ | 5′taacactcagacaggccacc | 134 bp |
PRDX-6 (NM_004905.3) | 5′atgcctgtgacagctcgtgtg 3′ | 5′tcttcttcagggatggttgg3′ | 212 bp |
HIF-1α (NM_001530.4) | 5′tttccttctcttctccgcgtg 3′ | 5′ggctgcatctcgagactttt3′ | 175 bp |
GAPDH (NM_002046.7) | 5′agccacatcgctcagacac 3′ | 5′gcccaatacgaccaaatcc3′ | 65 bp |
Antibody | Brand | Source | Dilution | Antibody | Brand | Source | Dilution |
---|---|---|---|---|---|---|---|
anti-PRDX 1 | Pierce (PA5-29830) | Rabbit | 1:3000 | anti-iNOS | Pierce (MA3-030) | Mouse | 1:250 |
anti-PRDX 2 | Proteintech (60202-1-Ig) | Mouse | 1:5000 | anti-TNFα | Proteintech (60291-1-Ig) | Mouse | 1:100 |
anti-PRDX 3 | Proteintech (10664-1-AP) | Rabbit | 1:2000 | anti-pNF-κB-p65 | Pierce (MA5-15160) | Rabbit | 1:500 |
anti-PRDX 4 | Pierce (PA5-34853) | Mouse | 1:1000 | anti-HIF-1α | Pierce (MA1-16511) | Mouse | 1:500 |
anti-PRDX 5 | Proteintech (17724-1-AP) | Rabbit | 1:1000 | anti-tTG | Proteintech (15100-1-AP) | Rabbit | 1:2000 |
anti-PRDX 6 | Pierce (H00009588-M01) | Rabbit | 1:1000 | Anti-p53 | Pierce (MA5-12557) | Mouse | 1:100 |
anti-HIF-1α | Novus Biological (MA1-16511) | Mouse | 1:500 | Anti-cleaved caspase 3 | Cell Signalling (9661) | Rabbit | 1:1000 |
anti-β-Actin | Invitrogen (15G5A11/E2) | Mouse | 1:10,000 |
Gene | Control | CD |
---|---|---|
PRDX-1 | 1.105 ± 0.21 | 0.203 ± 0.05 * |
PRDX-2 | 1.587 ± 0.41 | 0.566 ± 0.1 * |
PRDX-3 | 1.32 ± 0.47 | 0.987 ± 0.29 |
PRDX-4 | 1.135 ± 0.31 | 0.407 ± 0.13 * |
PRDX-5 | 1.484 ± 0.58 | 7.832 ± 2.68 * |
PRDX-6 | 1.226 ± 0.29 | 0.93 ± 0.22 |
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Köse, F.A.; Pabuccuoglu, A.; Karakoyun, M.; Aydogdu, S. Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients. Curr. Issues Mol. Biol. 2023, 45, 1779-1793. https://doi.org/10.3390/cimb45020114
Köse FA, Pabuccuoglu A, Karakoyun M, Aydogdu S. Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients. Current Issues in Molecular Biology. 2023; 45(2):1779-1793. https://doi.org/10.3390/cimb45020114
Chicago/Turabian StyleKöse, Fadime Aydın, Aysun Pabuccuoglu, Miray Karakoyun, and Sema Aydogdu. 2023. "Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients" Current Issues in Molecular Biology 45, no. 2: 1779-1793. https://doi.org/10.3390/cimb45020114