Effect of High-Intensity Interval Training on Cardiac Apoptosis Markers in Methamphetamine-Dependent Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Animals
2.2. METH Injection
2.3. Exercise Training Protocol
2.4. Tissue Extraction
2.5. Primer Design and Synthesis
2.6. RNA Extraction and cDNA Synthesis
2.7. Real Time RT-PCR
2.8. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Weeks | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
---|---|---|---|---|---|---|---|---|
Number of intervals | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
Effort duration (min) | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
Effort velocity (m/min) | 22 | 24 | 26 | 28 | 30 | 32 | 34 | 36 |
Rest duration | 2 | 2 | 3 | 3 | 4 | 4 | 4 | 4 |
Rest velocity (m/min) | 10 | 10 | 11 | 11 | 12 | 12 | 13 | 13 |
Gene Name | Forward Primer | Reverse Primer |
---|---|---|
Caspase-3 | 5′-GCAGCAGCCTCAAATTGTTGACTA-3′ | 5′-TGCTCCGGCTCAAACCATC-3′ |
Melusin | 5′-GGGTGAAGGCCAGTCAAACT-3′ | 5′-TGCTCCACGTTTATGACCCC-3′ |
FAK | 5′-CTTAATCTGGCCAGGACGGT-3′ | 5′-GAAGCACGGTTTGAGAGGTG-3′ |
IQGAP1 | 5′-ACAATCTGGAGACGCAAGCA-3′ | 5′-AGCTGCTCTCGGTTATACGC-3′ |
GAPDH | 5′-CAACTCCCTCAAGATTGTCAGCAA-3′ | 5′-GGCATGGACTGTGGTCATGA-3′ |
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Shahrabadi, H.; Haghighi, A.H.; Askari, R.; Asadi-Shekaari, M.; Souza, D.C.; Gentil, P. Effect of High-Intensity Interval Training on Cardiac Apoptosis Markers in Methamphetamine-Dependent Rats. Curr. Issues Mol. Biol. 2022, 44, 3030-3038. https://doi.org/10.3390/cimb44070209
Shahrabadi H, Haghighi AH, Askari R, Asadi-Shekaari M, Souza DC, Gentil P. Effect of High-Intensity Interval Training on Cardiac Apoptosis Markers in Methamphetamine-Dependent Rats. Current Issues in Molecular Biology. 2022; 44(7):3030-3038. https://doi.org/10.3390/cimb44070209
Chicago/Turabian StyleShahrabadi, Hadi, Amir Hossein Haghighi, Roya Askari, Majid Asadi-Shekaari, Daniel Costa Souza, and Paulo Gentil. 2022. "Effect of High-Intensity Interval Training on Cardiac Apoptosis Markers in Methamphetamine-Dependent Rats" Current Issues in Molecular Biology 44, no. 7: 3030-3038. https://doi.org/10.3390/cimb44070209