Evaluation of BCL6 and SIRT1 as Non-Invasive Diagnostic Markers of Endometriosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Preparation
2.2. Sample Processing and Storage
2.3. ELISA Procedure
2.4. Data Analysis
3. Results
3.1. BCL6 ELISA
3.2. SIRT1 ELISA
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Categories | Inclusion Criteria | Exclusion Criteria |
---|---|---|
Age, years | 18–42 | >42 |
Menopausal Status | Premenopausal | Postmenopausal |
Menstruation Status | Cycle frequency 21–35 days | Cycle < 21 days or >35 days |
Past Medical History * | NA | PCOS, psychiatric history, diabetes, known communicable infections |
Medications | NA | Hormonal therapy in the past 3 months |
Pregnancy Status | None | Pregnant |
Surgery Type | Gynecological ** | Oncologic |
Surgery, Count | Control Group | Stage I/II | Stage III/IV |
---|---|---|---|
Adhesion Lysis | 0 | 0 | 2 |
Adnexal Removal | 1 | 0 | 0 |
Chromotubation | 0 | 1 | 1 |
Dilation and Curettage | 0 | 0 | 1 |
Endometriosis Resection | 2 | 6 | 3 |
Hysterectomy | 1 | 2 | 1 |
Myomectomy | 0 | 3 | 0 |
Ovarian Cystectomy | 1 | 0 | 2 |
Oviduct Fulguration | 2 | 0 | 0 |
Salpingectomy | 2 | 0 | 0 |
Characteristic | Control | I/II | III/IV |
---|---|---|---|
Age, mean (SD) | 29.7 (4.9) | 34.5 (5.8) | 31.6 (3.5) |
Race, % | |||
African American | 40 | 20 | 0 |
Asian | 0 | 0 | 10 |
White | 50 | 60 | 90 |
White/Hispanic | 0 | 10 | 0 |
Hispanic | 10 | 0 | 0 |
Unknown | 0 | 10 | 0 |
Value Category | SIRT1 | BCL6 |
---|---|---|
Concentration—pg/mL, mean (SD) * | ||
Negative Controls | 1141.7 (655.4) | 1953.8 (554.0) |
Stage I/II Endometriosis | 932.3 (466.3) | 1645.7 (665.9) |
Stages III/IV Endometriosis | 2357.5 (1274.5) | 2000.5 (552.4) |
t-test Results | ||
Negative Controls vs. Stages I/II | 0.4213 | 0.2754 |
Negative Controls vs. Stages III/IV | 0.0152 ** | 0.8525 |
Stages I/II vs. Stages III/IV | 0.0038 ** | 0.2111 |
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Sansone, A.M.; Hisrich, B.V.; Young, R.B.; Abel, W.F.; Bowens, Z.; Blair, B.B.; Funkhouser, A.T.; Schammel, D.P.; Green, L.J.; Lessey, B.A.; et al. Evaluation of BCL6 and SIRT1 as Non-Invasive Diagnostic Markers of Endometriosis. Curr. Issues Mol. Biol. 2021, 43, 1350-1360. https://doi.org/10.3390/cimb43030096
Sansone AM, Hisrich BV, Young RB, Abel WF, Bowens Z, Blair BB, Funkhouser AT, Schammel DP, Green LJ, Lessey BA, et al. Evaluation of BCL6 and SIRT1 as Non-Invasive Diagnostic Markers of Endometriosis. Current Issues in Molecular Biology. 2021; 43(3):1350-1360. https://doi.org/10.3390/cimb43030096
Chicago/Turabian StyleSansone, Alison M., Brooke V. Hisrich, R. Brandt Young, William F. Abel, Zachary Bowens, Bailey B. Blair, Avery T. Funkhouser, David P. Schammel, Lisa J. Green, Bruce A. Lessey, and et al. 2021. "Evaluation of BCL6 and SIRT1 as Non-Invasive Diagnostic Markers of Endometriosis" Current Issues in Molecular Biology 43, no. 3: 1350-1360. https://doi.org/10.3390/cimb43030096
APA StyleSansone, A. M., Hisrich, B. V., Young, R. B., Abel, W. F., Bowens, Z., Blair, B. B., Funkhouser, A. T., Schammel, D. P., Green, L. J., Lessey, B. A., & Blenda, A. V. (2021). Evaluation of BCL6 and SIRT1 as Non-Invasive Diagnostic Markers of Endometriosis. Current Issues in Molecular Biology, 43(3), 1350-1360. https://doi.org/10.3390/cimb43030096