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Brief Report

Oxidized Cell-Free DNA Rapidly Skews the Transcriptional Profile of Brain Cells toward Boosting Neurogenesis and Neuroplasticity

1
Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia
2
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
3
Department of Normal Physiology, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Emiel P.C. van der Vorst
Curr. Issues Mol. Biol. 2021, 43(3), 1583-1591; https://doi.org/10.3390/cimb43030112
Received: 9 September 2021 / Revised: 5 October 2021 / Accepted: 9 October 2021 / Published: 13 October 2021
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
Cell-free DNA (cfDNA) is liberated and accumulated in neural tissue due to cell damage. The oxidative and nitrosative stress in the brain that accompanies various pathological conditions has been shown to increase the oxidation of cellular and cell-free DNA. Whether the high concentration of non-oxidized and oxidized cfDNA may affect the transcriptome response of brain cells has not been studied. In the current work, we studied whether cfDNA fragments affect several key pathways, including neurogenesis, at the level of gene expression in brain cells. In the study, primary rat cerebellum cell cultures were used to assess the effects of oxidized and non-oxidized cfDNA on the expression of 91 genes in brain cells. We found that only oxidized cfDNA, not non-oxidized cfDNA, significantly altered the transcription in brain cells in 3 h. The pattern of change included all 10 upregulated genes (S100A8, S100A9, S100b, TrkB, Ngf, Pink1, Aqp4, Nmdar, Kcnk2, Mapk1) belonging to genes associated with neurogenesis and neuroplasticity. The expression of inflammatory and apoptosis genes, which oppose neurogenesis, decreased. The results show that the oxidized form of cfDNA positively regulates early gene expression of neurogenesis and neuroplasticity. At the same time, the question of whether chronic elevation of cfDNA concentration alters brain cells remains unexplored. View Full-Text
Keywords: neurogenesis; inflammation; oxidized cell-free DNA; mRNA neurogenesis; inflammation; oxidized cell-free DNA; mRNA
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MDPI and ACS Style

Filev, A.D.; Kostyuk, S.V.; Umriukhin, P.E.; Pisarev, V.M. Oxidized Cell-Free DNA Rapidly Skews the Transcriptional Profile of Brain Cells toward Boosting Neurogenesis and Neuroplasticity. Curr. Issues Mol. Biol. 2021, 43, 1583-1591. https://doi.org/10.3390/cimb43030112

AMA Style

Filev AD, Kostyuk SV, Umriukhin PE, Pisarev VM. Oxidized Cell-Free DNA Rapidly Skews the Transcriptional Profile of Brain Cells toward Boosting Neurogenesis and Neuroplasticity. Current Issues in Molecular Biology. 2021; 43(3):1583-1591. https://doi.org/10.3390/cimb43030112

Chicago/Turabian Style

Filev, Anton D., Svetlana V. Kostyuk, Pavel E. Umriukhin, and Vladimir M. Pisarev 2021. "Oxidized Cell-Free DNA Rapidly Skews the Transcriptional Profile of Brain Cells toward Boosting Neurogenesis and Neuroplasticity" Current Issues in Molecular Biology 43, no. 3: 1583-1591. https://doi.org/10.3390/cimb43030112

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