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Assessment of Bioavailability after In Vitro Digestion and First Pass Metabolism of Bioactive Peptides from Collagen Hydrolysates

School of Human Nutrition, McGill University, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada
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Academic Editors: Neuman Manuela and Stephen Malnick
Curr. Issues Mol. Biol. 2021, 43(3), 1592-1605; https://doi.org/10.3390/cimb43030113
Received: 27 September 2021 / Revised: 7 October 2021 / Accepted: 9 October 2021 / Published: 13 October 2021
Collagen hydrolysates (CHs) are composed of bioactive peptides (BAPs), which possess health enhancing properties. There is a knowledge gap regarding the bioavailability of these BAPs that involves intestinal transport and hepatic first pass effects. A simulated gastrointestinal model was used to generate digesta from two CHs (CH-GL and CH-OPT), which were applied to a novel transwell co-culture of human intestinal epithelium cell line-6 (HIEC-6) and hepatic (HepG2) cells to simulate in vivo conditions of absorption and first pass metabolism. Peptide transport, hepatic first pass effects, and bioavailability were determined by measuring BAPs (Gly-Pro, Hyp-Gly, Ala-Hyp, Pro-Hyp, Gly-Pro-Hyp) using an innovative capillary electrophoresis method. All peptides were transported across the intestinal cell layer to varying degrees with both CHs; however, Gly-Pro-Hyp was transported only with CH-GL, but not CH-OPT. Notable hepatic production was observed for Ala-Hyp with both CH treatments, and for Pro-Hyp and Gly-Pro with CH-GL only. All peptides were bioavailable (>10%), except for Gly-Pro-Hyp after CH-OPT. Overall, a high degree of transport and hepatic first pass effects on CH-derived BAPs were observed. Further research is needed to explore the hepatic mechanisms related to the production of BAPs and the bifunctional effects of the bioavailable BAPs noted in this study. View Full-Text
Keywords: bioavailability; digestion; bioactive peptides; first pass metabolism; collagen hydrolysate; cell culture; capillary electrophoresis; human intestinal epithelial cells (HIEC-6); permeability bioavailability; digestion; bioactive peptides; first pass metabolism; collagen hydrolysate; cell culture; capillary electrophoresis; human intestinal epithelial cells (HIEC-6); permeability
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MDPI and ACS Style

Larder, C.E.; Iskandar, M.M.; Kubow, S. Assessment of Bioavailability after In Vitro Digestion and First Pass Metabolism of Bioactive Peptides from Collagen Hydrolysates. Curr. Issues Mol. Biol. 2021, 43, 1592-1605. https://doi.org/10.3390/cimb43030113

AMA Style

Larder CE, Iskandar MM, Kubow S. Assessment of Bioavailability after In Vitro Digestion and First Pass Metabolism of Bioactive Peptides from Collagen Hydrolysates. Current Issues in Molecular Biology. 2021; 43(3):1592-1605. https://doi.org/10.3390/cimb43030113

Chicago/Turabian Style

Larder, Christina E., Michèle M. Iskandar, and Stan Kubow. 2021. "Assessment of Bioavailability after In Vitro Digestion and First Pass Metabolism of Bioactive Peptides from Collagen Hydrolysates" Current Issues in Molecular Biology 43, no. 3: 1592-1605. https://doi.org/10.3390/cimb43030113

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