Next Article in Journal
Oxidized Cell-Free DNA Rapidly Skews the Transcriptional Profile of Brain Cells toward Boosting Neurogenesis and Neuroplasticity
Previous Article in Journal
Molecular Profiling of DNA Methylation and Alternative Splicing of Genes in Skeletal Muscle of Obese Rabbits
Communication

Gene Expression Analysis with No Sequence Data: Study on Reeves’s Muntjac (Muntiacus reevesi)

Department of Animal Nutrition and Biotechnology, and Fisheries, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Juozas Lazutka
Curr. Issues Mol. Biol. 2021, 43(3), 1576-1582; https://doi.org/10.3390/cimb43030111
Received: 18 September 2021 / Revised: 7 October 2021 / Accepted: 8 October 2021 / Published: 12 October 2021
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
Despite scientific progress, the gene sequences for many species not commonly used in research have not yet been analyzed. This makes it difficult to carry out molecular studies on such animals, as the sequence of genes is the basic information used in many techniques. In this study, we attempt to design primers for a real-time PCR analysis, basing on a comparative analysis of selected gene sequences of species related to Reeves’s muntjac (Muntiacus reevesi) and by identifying highly conservative regions. Results of PCR products sequencing and their alignment with the GenBank collection show that all selected primers gave products highly similar (> 90%) to the intended target (among compared species), which led us to the conclusion that our primers may be used for further analyses of gene expression. View Full-Text
Keywords: primer designing; gene sequence; mRNA expression primer designing; gene sequence; mRNA expression
MDPI and ACS Style

Flaga, J.; Przybyło, M.; Górka, P. Gene Expression Analysis with No Sequence Data: Study on Reeves’s Muntjac (Muntiacus reevesi). Curr. Issues Mol. Biol. 2021, 43, 1576-1582. https://doi.org/10.3390/cimb43030111

AMA Style

Flaga J, Przybyło M, Górka P. Gene Expression Analysis with No Sequence Data: Study on Reeves’s Muntjac (Muntiacus reevesi). Current Issues in Molecular Biology. 2021; 43(3):1576-1582. https://doi.org/10.3390/cimb43030111

Chicago/Turabian Style

Flaga, Jadwiga, Marcin Przybyło, and Paweł Górka. 2021. "Gene Expression Analysis with No Sequence Data: Study on Reeves’s Muntjac (Muntiacus reevesi)" Current Issues in Molecular Biology 43, no. 3: 1576-1582. https://doi.org/10.3390/cimb43030111

Find Other Styles

Article Access Map by Country/Region

1
Back to TopTop