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Review

Adoptive Immunotherapy for Hematological Malignancies Using T Cells Gene-Modified to Express Tumor Antigen-Specific Receptors

by
Hiroshi Fujiwara
Department of Hematology, Clinical Immunology and Infectious Disease, Graduate School of Medicine, Ehime University, Toon, Ehime 791-0295, Japan
Pharmaceuticals 2014, 7(12), 1049-1068; https://doi.org/10.3390/ph7121049
Submission received: 3 November 2014 / Revised: 26 November 2014 / Accepted: 8 December 2014 / Published: 15 December 2014
(This article belongs to the Special Issue Cell Therapy)
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Abstract

:
Accumulating clinical evidence suggests that adoptive T-cell immunotherapy could be a promising option for control of cancer; evident examples include the graft-vs-leukemia effect mediated by donor lymphocyte infusion (DLI) and therapeutic infusion of ex vivo-expanded tumor-infiltrating lymphocytes (TIL) for melanoma. Currently, along with advances in synthetic immunology, gene-modified T cells retargeted to defined tumor antigens have been introduced as “cellular drugs”. As the functional properties of the adoptive immune response mediated by T lymphocytes are decisively regulated by their T-cell receptors (TCRs), transfer of genes encoding target antigen-specific receptors should enable polyclonal T cells to be uniformly redirected toward cancer cells. Clinically, anticancer adoptive immunotherapy using genetically engineered T cells has an impressive track record. Notable examples include the dramatic benefit of chimeric antigen receptor (CAR) gene-modified T cells redirected towards CD19 in patients with B-cell malignancy, and the encouraging results obtained with TCR gene-modified T cells redirected towards NY-ESO-1, a cancer-testis antigen, in patients with advanced melanoma and synovial cell sarcoma. This article overviews the current status of this treatment option, and discusses challenging issues that still restrain the full effectiveness of this strategy, especially in the context of hematological malignancy.

1. Introduction

Clinically available adoptive immunotherapies employing non-gene-modified T cells have successfully demonstrated the potential for cure of certain cancers and viral infections. Examples include the graft-vs-leukemia (GVL) effect mediated by donor lymphocyte infusion (DLI) for treatment of relapsed leukemia and persistent reactivation of latent viruses after allogeneic stem cell transplantation (allo-HSCT) [1,2], and therapeutic infusion of ex vivo-expanded tumor-infiltrating lymphocytes (TIL) in combination with lymphodepletion for treatment of advanced melanoma [3,4]. This lymphodepleting preconditioning augments the antitumor response by eliminating regulatory T cells, by reducing total T cells which are a “sink” for cytokines including IL-7 and IL-15 playing important roles in “homeostatic expansion”, and by augmenting the activation and availability of antigen-presenting cells [5,6,7]. However, both DLI and TIL therapy have fundamental drawbacks; the former is usually associated with a potentially life-threatening form of allo-immunity-mediated damage to normal tissue, graft-vs-host disease (GVHD) [8], whereas the latter requires time-consuming and labor-intensive procedures for preparation of therapeutic cells, and the results are sometimes unsuccessful [9]. Separation of the GVL effect from GVHD still remains the main challenge in allo-HSCT, and to achieve this, attempts have been made to discover effective immunogenic antigens that can be selectively or abundantly expressed in leukemia cells, but not in normal tissues [10].
In order to overcome these drawbacks, the concept of genetically modifying naturally occurring effector T cells so that they become specifically responsive to therapeutic targets has been developed as an alternative approach. At present, gene-modified T cell-based adoptive immunotherapy is still evolving in order to achieve durable suppression of cancer progression, which is prerequisite for cure. Preclinical and clinical studies using adoptive T-cell transfer have revealed that cytotoxic CD8+ T cells (CTLs) are primarily advantageous in comparison with other cytolytic cells, such as NK cells, because they are capable of specifically targeting tumor cells through recognition of differentially expressed cell surface antigens, and also clonal proliferation after target recognition, resulting in durable suppression of cancer cells [11]. The long life span of clonal T cells also seems attractive from the viewpoint of not only therapeutics, but also immuno-prophylaxis to durably reduce the incidence of disease in patients with a high risk of cancer [12]. As is the case with TIL therapy, the anticancer efficacy of infused gene-modified T cells can also be enhanced using lymphodepletion [13]. Furthermore, T cells are able to tolerate gene modification using viral vectors and transposons [14]. This makes T cells suitable for transduction with target antigen-specific receptor genes, making it possible to uniformly redirect large numbers of polyclonal T cells towards cancer cells on a large scale. In the context of these technological advances, adoptive immunotherapy using genetically retargeted T cells has become a realistic treatment option for cancer and refractory viral infection [15]. The present review summarizes the current status of anticancer adoptive immunotherapy using gene-modified T cells, and discusses the various issues that still blunt the full effectiveness of this strategy, particularly for hematological malignancy.

2. Gene-Modified T Cells Redirected Towards Therapeutic Targets

Through the development of anti-melanoma TIL therapy [3,4] employing ex vivo-expanded T cells with guaranteed tumor reactivity and tumor trafficking activity, several requisite conditions for a favorable clinical outcome of adoptive T-cell immunotherapy have become apparent: (i) the choice of an optimal target antigen; (ii) to the extent possible guaranteed uniformity of the quantity and quality of the therapeutic T cells administered; and (iii) tumor trafficking and in vivo persistence of the infused T cells [16]. Ideal effector T cells should bear a single T-cell receptor with high affinity optimized to an appropriately selected target antigen, and should be capable of actively proliferating upon recognition of the target cells, durably persisting thereafter in vivo. In addition, such effector T cells need to be prepared in sufficient numbers in a timely manner.
At present, retro- or lentiviral, or electroporational transfer of chimeric antigen receptors (CARs) whose target recognition is dependent on a single-chain variable region domain of a monoclonal antibody (scFv) or that of a T-cell receptor (TCR) is employed for stable production of sufficient numbers of therapeutic T cells (CAR-T cells or TCR-T cells, respectively) uniformly retaining specificity for their defined targets, generally in 2-week culture [15]. Accordingly, the concept that, in the near future, therapeutic gene-modified T cells may be employed an “off-the-shelf” cellular drug and produced on an industrial scale is becoming accepted worldwide, encouraged by proactive measures by the US Food and Drug Administration in balancing the benefits and risks to subjects in clinical trials [17]. Figure 1 shows a flow chart of adoptive immunotherapy using gene-modified T cells.
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Figure 1. Flow chart of antileukemia adoptive immunotherapy using gene-modified T cells (modified from [18]).
Figure 1. Flow chart of antileukemia adoptive immunotherapy using gene-modified T cells (modified from [18]).
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PBL harvested from a patient with leukemia in CR or a HLA-identical allo-HSCT donor using leukapheresis are subjected to gene modification with a relevant TCR or CAR. Subsequently, TCR-T cells or CAR-T cells are expanded on a clinical scale at a cell processing center. A sufficient number of TCR-T or CAR-T cells are then infused back into the same patient, with or without chemo-radiotherapeutic preconditioning. PBL: peripheral blood lymphocytes, CR: complete remission, TCR: T cell receptor, CAR: chimeric antigen receptor, TCR-T: T cell receptor gene-transduced T cells, CAR-T : chimeric antigen receptor gene-transduced T cells, CPC: Cell processing center, Pt: patient, DNR: donor, CTx: chemotherapy.
Given that allo-HSCT, being the most successful type of adoptive therapy, requires timely acquisition of hematopoietic stem cells from the more appropriate donor, the gene-modification using autologous lymphocytes from cancer-bearing patients, not requiring donors, can provide the higher clinical versatility. On the other hand, gene-modification of allogeneic T cells from an allo-HSCT donor for DLI is greatly advantageous to employ chemo-naïve healthy T cells. In Figure 2, the basic structure of TCR-T and CAR-T cells is shown [18].
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Figure 2. A T cell gene-modified to express a TCR or CAR (modified from [18]).
Figure 2. A T cell gene-modified to express a TCR or CAR (modified from [18]).
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The introduced TCR (left) or CAR (right) construct in a gene-modified T cell is illustrated. The TCR recognizes the epitope derived from cytoplasmic antigens in the context of the particular HLA (left), whereas the CAR recognizes the cell surface antigen on the basis of the recognition machinery of a monoclonal antibody, independently of HLA (right). TCR: T cell receptor, CAR: chimeric antigen receptor, HLA: human leukocyte antigen, Vα: variable region of the TCR α-chain, Vβ: variable region of the TCR β-chain, Cα: constant region of the TCR α-chain, Cβ: constant region of the TCR β-chain, ε, γ, δ, ζ: each subunit of the CD3 molecule, Ag: antigen, VL: variable region of the immunoglobulin light chain, VH: variable region of the immunoglobulin heavy chain, ITAM: immunoreceptor tyrosine-based activation motif.
The TCR-T cell, but not the CAR-T cell, requires a specific HLA molecule for recognition of the target antigen which is processed within the cytoplasm and presented on the surface of cancer cells, i.e., HLA restriction. In other words, limited individuals who inherently have particular HLA molecules are eligible for this treatment option. Compared to CAR-T cells, such HLA restriction limits clinical versatility for TCR-T cells to some extent, however TCR-T cells, but not CAR-T cells, have the ability to recognize intracellular proteins, providing a broader array of target tumor-associated antigens [19].
The therapeutic quality of gene-modified TCR-T cells is primarily dependent on their avidity. Because of thymic selection during the development of T cells and the fact that except for a few mutated proteins encoded by cancer-causing genetic mutations (driver mutations), a large proportion of tumor antigens are self-antigens, and circulating T cells have already been massively exposed to tumor antigens in cancer-bearing patients, naturally occurring TCRs expressed on circulating T cells generally have low affinity for self-antigens (Kd range 1–100 μM) [20]. This makes such circulating T cells less responsive to autologous cancer cells, because generally cancer cells tend to express small amounts of epitope/HLA complexes on their surface [21]. Therefore, to create higher avidity, several strategies have been developed with the aim of increasing the affinity of the relevant TCR beyond that expressed by naturally occurring T cells in response to an identical epitope. These strategies include the use of selected TCRs from immunized human HLA transgenic mice with relevant epitopes and insertion of targeted mutation in complementary-determining region 2 or 3 (CDR2 or 3) in the variable regions of the TCR α/β chains that interact with the HLA/epitope complex [22,23,24,25,26]. For example, a mutation encodes Alanine to Threonine amino acid substitution in CDR3 region of the TCR-α chain of MAGE-A3-specific TCR at position 118 is inserted [25]. Such affinity-increased TCRs have already been employed in clinical trials [25,26,27,28,29]. Those affinity-increased TCRs that were included figure in Table 1 and are highlighted with *. Six of 20 patients with advanced melanoma treated with gene-modified T cells using a HLA-A*0201-restricted melan-A protein and melanoma antigen recognized by T cells 1 (MART-1)-specific TCR achieved partial remission (PR), those were suffered from dermatitis and uveitis (grade2) [27]. One of three patients with advanced colorectal carcinoma treated with gene-modified T cells using a HLA-A*0201-restricted carcinoenbryonic antigen (CEA)-specific TCR showed PR, but three of three patients contracted treatment-required colitis (grade 3) [28]. Two of 11 patients with advanced melanoma HLA-A*0201-restricted NY-ESO-1-specific TCR demonstrated complete remission (CR) and three of 11 achieved PR. Additionally four of six patients with metastatic synovial cell carcinoma treated with the same NY-ESO-1-specific TCR achieved PR. None of the patients demonstrated any adverse events [29]. Five of nine patients (seven patients with advanced melanoma, one metastatic synovial cell carcinoma and one advanced esophageal carcinoma) treated with gene-modified T cells using a HLA-A*0201-restricted melanoma antigen family A3 (MAGE-A3)-specific TCR demonstrated tumor regression, but two patients died of severe central nervous system damage [25]. Two of two patients (one advanced melanoma and one advanced myeloma) treated with gene-modified T cells using HLA-A*01-restricted TCR died of severe cardiac dysfunction shortly after T-cell infusion [26]. TCR-T-associated adverse events are discussed later.
On the other hand, it is widely accepted that introduction of a therapeutic TCR can create an unwanted α/β heterodimer between the introduced and endogenous TCR α/β chains (mispairing). Mispairing can directly reduce the cell-surface expression of intended TCR α/β pairing, resulting in low avidity. In turn, cancer cells in daily practice show low cell surface expression of the relevant epitope/HLA complex, accordingly gene-modified T cells expressing mispaired TCRs seem apt to fail to recognize cancer cells [30,31].
Furthermore, the potential risk of severe autoimmune disease caused by newly generated mispaired TCR with unpredictable auto-responsiveness has been highlighted in a mouse model [32], although so far no such adverse event has been observed in clinical trials. To circumvent mispaired TCR formation, knockdown or knockout of endogenous TCR-α/β gene expression has recently been introduced using siRNA technology (siTCR vector) [33,34] or zinc-finger nuclease (ZFN) technology [35,36].
Table
Table 1. Results from clinical trials using TCR-T cells.
Table 1. Results from clinical trials using TCR-T cells.
Taregt Ag. of TCRCell Dose (× 109)Target Disease Pt.No. (n)PreconditioningAEs (Grade)Clinical ResponsesRef.
MART-11.0–86melanoma Cy + FludNoPR 2/17[37]
(n = 17)
MART-1*1.5–107melanoma (20)Cy + Fludskin, eye (G2)PR 6/20[27]
gp100**1.8–110melanoma (16) ear (G3)CR 1/16,PR 2/16
(n = 36)
p53**/gp100**0.5–27.7breast ca. (4)Cy + FludN/APR 1/9[38]
melanoma (2)
esoph.ca. (1)
others (2)
CEA*0.2–0.4colorectal ca.Cy + Fludcolitis (G3)PR 1/3[28]
(n = 3) CEA decreased 3/3
NY-ESO-1*1.6–130melanoma (11)Cy + FludNoCR 2/11, PR 3/11[29]
synovial cell ca.(6)PR 4/6
(n = 17)
MAGE-A3*29–79melanoma (7)Cy + Fludmental disturbance (G4)Tumor regression[25]
synovial cell ca.(1)2/3 died of necrotizing5/9
esoph.ca. (1)leukoencephalopathy
(n = 9)(on-target AE)
MAGE-A3*5.3 & 2.4melonoma (1)Cy2/2 died of cardiogenicNE[26]
myeloma (1)melphalan + autoSCTshock
(n = 2) (off-target AE)
Abbreviations: Ag: antigen; AE: adverse events; Ref.: reference; Cy: cyclophosphamide; Flud: fludarabine; PR: partial response; CR: complete remission by response evaluation criteria in solid tumors (RECIST) *: affinity-increased TCR; **: mouse-derived high-avidity TCR; breast ca.: breast cancer; esoph.ca.: esophageal cancer; synovial cell ca.: synovial cell carcinoma; N/A; not available; N/E: not evaluable; autoSCT: autologous stem cell transplant.
siTCR vector simultaneously transduces therapeutic TCR-α/β genes and siRNA for constant regions of endogenous TCR-α/β genes for the suppression in gene-modified T cells. Therapeutic TCR-α/β genes were codon-optimized to be resistant to those siRNAs. Accordingly, mispairing between introduced and endogenous TCR-α/β genes can be reduced. In cooperation with Takara Bio Inc. and Mie University, we have originally developed the siTCR vector system, and recently launched a clinical trial using a therapeutic siTCR vector targeting a leukemia antigen, Wilms Tumor 1 (WT1-siTCR vector) [39], for patients with acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS) (UMIN 00001159). Silencing of the CC chemokine 5 (CCR5) gene in CD4+ T cells using ZFN technology has also been used in a clinical trial for patients with human immunodeficiency virus (HIV) infection (NCT00842634, ClinicalTrials.gov.Identifier), and shown to be clinically safe [40]. However, at the time of writing, no clinical trial of anticancer adoptive immunotherapy using ZFN –manipulated T cells had been reported.
As shown in Figure 2, the CAR construct is composed of an extracellular antigen-binding domain, a transmembrane domain, and a cytoplasmic signaling domain [41]. First-generation CAR constructs solely containing either CD3-ζ or FcR-γ as a cytoplasmic signaling molecule [42,43] were clinically unsuccessful [44]. Limitations in the proliferative response following recognition of tumor cells, and in persistence and homing of first-generation CAR-T cells to local tumor cells in vivo have been largely surmounted by second-generation CAR constructs containing co-stimulatory signaling molecules such as CD28 [45], 4-1BB [46], OX40 [47] and ICOS (inducible T-cell co-stimulator; CD278) [48], resulting in upregulation of anti-apoptotic factors and increased secretion of cytokines upon antigen recognition [49,50]. Consequently, clinical outcomes resulting from the use of second-generation CAR-T cells have been dramatically improved, particularly for B-cell malignancy [51,52,53,54,55,56]. For further upregulation of antitumor functionality mediated by CAR-T cells, third-generation CAR constructs are currently being investigated [41]. On the other hand, a better understanding of the extracellular domain is still emerging [57]. In addition, as was the case with TCR-T cells [4], preconditioning for lymphodepletion prior to transfer of CAR-T cells also seems to affect the functionality of these effector cells [58].

3. Lessons from Clinical Trials Using T Cells Gene-Modified by Tumor Antigen-Specific Receptor Gene Transfer

Results from major clinical trials using TCR-T cells and CAR-T cells are summarized separately in Table 1 and Table 2. Promising outcomes have been achieved in clinical trials using CD19-specific CAR constructs for patients with refractory chronic lymphocytic leukemia [51,52,54,59], B-cell [53,55,59,60], and acute lymphoblastic leukemia [54,61]. Interestingly, more impressive clinical benefits seemed to be achieved using the CD19-CAR construct containing 4.1BB in the signaling motif [51,52,61] than with the use of CD28 [53,54], although the mechanism underlying this difference in antitumor efficacy is not fully understood. Even if the same CD19 is targeted, because of the differences in the cytoplasmic domain and the extracellular domain recognizing a different epitope of the same antigen with differing affinity for each CAR construct, the antitumor functionality mediated by CAR-T cells varies [62]. As to the importance of the extracellular domain, studies of the anti-CD22 CAR construct have revealed that the extent of antitumor efficacy mediated by each anti-CD22 CAR-T cell widely varies according to the individual extracellular domain that recognizes a different epitope, even when derived from the same CD22 molecule [63].
Table
Table 2. Results from clinical trials using CAR-T cells.
Table 2. Results from clinical trials using CAR-T cells.
Target Ag. of CARCell DoseTarget disease Pt.No. (n)PreconditioningAEs (Grade)Clinical ResponsesRef.
L1-cell adhesion1 × 108neuroblastoma (6)nonepancytopenia (G3)PR 1/6[64]
molecule/CAR*Or 109/m2 bacteremia, pneumonitis
HER2/CAR**1 × 101°colon cancer withCy + Fluddied of acute pulmonaryN/E[65]
lung/liver meta. (1)failure
GD2/CAR*0.2–0.5–1neuroblastoma (19)nonenoCR 3/19, PR 1/19[66]
EBV-CTL× 108
CD19/CAR***1.1 × 109CLL (3)CTx for CLLlymphopenia (G3)CR[51]
5.8 × 108 B cell aplasiaPR
1.4 × 107 CR
CD19/CAR#1.0–11.1CLL (8)none for 3hypotension (G3)PR 1/8 in CLL[53]
× 109ALL (2)Cy (1500 mg or 3000 mg)1 died of shock, renal failureB cell aplasia
for othersB cell aplasia
CD19/CAR#0.5–5.5CLL (4)Cy + Fludhypotension (G3/4)CR 1/4, PR 2/4 in CLL[52]
× 107 /KgFL (4) renal failure, infectionPR 3/4 in FL
B cell aplasiaB cell aplasia
CD20/CAR**4.4 × 109 /m2MCL (2)Cy (1000 mg/m2 )hypoxia (G3), fever (G2)PR in FL[67]
FL (1)
CD19/CAR#1.0–11.1B-ALL (5)Cy (1500 mg or 3000 mg)fever (G2)CR 2/6 (no MRD)[54]
× 106 /Kg
CD19/CAR***1.4–12ALL (2)CTx for ALLCRS (G3-4)CR 2/2[55]
× 106 /Kg B cell aplasia
CD19/CAR #3 × 106 /kgrefractory B-ALL (16)Cy (1500 mg or 3000 mg)CRS (G3-4) (7/16)CR 14/16[56]
ph+ (4/16)neurologic complication (3/7)molecular CR 10/14
respiratory ventilation (3/3)transit to allo-HSCT
(7/14)
Abbreviations: Ag.: antigen; AE: adverse events; Ref.: reference; Cy: cyclophosphamide; Flud: fludarabine; PR: partial response; CR: complete remission; CTx: chemotherapy; CLL: chronic lymphocytic leukemia; (B-)ALL: (B-cell) acute lymphoblastic leukemia; MCL: mantle cell lymphoma; FL: follicular cell lymphoma; HER2: human epitherial growth factor receptor 2; EBV: Epstein-Barr virus; CTL: cytotoxic T lymphocyte; *: first generation CAR: **: CD28,4.1BB bearing CAR; ***: 4.1BB bearing 2nd generation CAR; #: CD28 bearing 2nd generation CAR; N/A: not available; N/E: not evaluable: CRS: cytokine releasing syndrome; allo-HSCT: allogeneic hematopoietic stem cell transplantation.
Nonetheless, the contribution of high-performance 2nd and 3rd generation CAR constructs to the clinical success of anti-CD19 CAR-T cell therapy for B cell malignancy is largely attributable to the nature of CD19 itself: (i) CD19 is expressed not only by leukemia cells, but also by normal antigen-presenting B cells that can provide co-stimulatory signals to CAR-T cells upon contact, (ii) infused CAR-T cells are easily accessible to leukemia cells in the same physiological compartment (lympho-vascular system), and (iii) apart from unknown late-phase adverse events [68], adverse symptoms associated with B-cell depletion appear to be clinically manageable using intravenous administration of immunoglobulin, i.e., B cells can be considered to be a relatively non-essential normal tissue. The clinical success of anti-CD19 CAR-T cell therapy again underlies the importance of choice of the optimal target antigen for gene-modified T cells.
On the other hand, with regard to TCR-T cell therapy, Rosenberg and colleagues have been playing a leading role, particularly in the treatment of advanced melanoma [27,37] and solid tumors [28]. They have recently reported impressive clinical outcomes with the use of a genetically mutated high-affinity TCR specific to NY-ESO-1 in the context of HLA-A*02:01, a representative cancer-testis antigen (CTA) that is overexpressed in various types of cancers, but negligibly expressed in almost all normal tissues except for testis [29]. Infusion of NY-ESO-1-specific TCR-T cells resulted in tumor regression in five of six patients with advanced melanoma, including two patients who achieved complete remission for longer than 1 year, and four of six patients with synovial cell carcinoma. There is a concern that a TCR expressing supra-physiologic affinity might have an increased risk to damage normal tissues that physiologically express the same target antigen even at a negligible level (on-target adverse effect), but not when NY-ESO-1 is targeted. Again, naturally occurring CTLs in the circulation of a cancer-bearing patient are less responsive to autologous cancer cells [21]. Of necessity, a technology for artificially upregulating the affinity of a relevant TCR to a level as high as that of a monoclonal antibody (Kd value < nM) has been developed. Accordingly, emerging on- and off-target adverse effects mediated by such artificial TCRs with supra-physiologic affinities have become a serious issue, and will be discussed later.
In the context of hematological malignancy, no clinical observations of TCR-T therapy have yet been reported. Currently, three clinical trials using TCR-T cells targeting WT1 are ongoing. The first is being conducted by our group in Japan using autologous gene-modified T cells with a naturally occurring HLA-A*24:02-restricted WT1-specific TCR using a siTCR vector without lymphodepletion for the treatment of AML/MDS (UMIN000011519). The second is being conducted by Greenberg and colleagues in the USA using allogeneic gene-modified T cells with a naturally occurring and selected high-affinity HLA-A*02:01-restricted WT1-specific TCR in combination with rhIL-2 administration, but without using lymphodepletion, for the treatment of relapsed or high-risk AML/MDS/CML after allo-HSCT (ClinicalTrials.gov. Identifier NCT01640301). This group has previously reported the results of a similar clinical trial using ex vivo-expanded allogeneic donor-derived CTL clones employing repetitive stimulation of the HLA-A*02:01-restricted WT1 epitope for treatment of relapsed or high-risk AML after allo-HSCT [69]. In the same study, infused allogeneic donor WT1-specific CTLs exerted an antileukemia effect without exacerbating GVHD, and those CTLs pre-cultured with a rhIL-21 expressing memory T-cell phenotype achieved a notably durable leukemia remission for longer than 30 months [69]. In the current clinical trial, gene-modified CD8+ T cells specific to the identical WT1 epitope in the context of HLA-A*02:01, in turn, are being employed. The third trial is being conducted by Morris and colleagues in the UK using autologous gene-modified T cells with a HLA-A*02:01-restricted WT1-specific TCR in combination with lymphodepletion for treatment of AML/CML (ClinicalTrials.gov. Identifier NCT01621724). Reports from these clinical trials are eagerly anticipated.

4. Adverse Effects of Gene-Modified T Cells

The adverse effects (AE)s caused by adoptively administered gene-modified T cells can largely be divided into: (i) on-target AE, which is damage due to the expression of target antigens in normal tissues recognized by the introduced CAR or TCR, and (ii) off-target AE, which is damaging to normal tissues that do not express the antigens targeted by the introduced CAR or TCR. Despite obvious tumor shrinkage, on-target AEs have been observed in a clinical trial using an avidity-increased HLA-A*0201-restricted TCR specific for the melanoma-associated antigens MART-1 and gp100, which are also expressed substantially in normal melanocytes and pigmented tissues, resulting in skin rash, hearing loss and uveitis [27]. When carcinoembryonic antigen (CEA), also constitutively expressed by normal colon epithelia, was targeted using T cells gene-modified to express a HLA-A*0201-restricted and CEA-specific high-avidity murine TCR, severe inflammatory colitis was observed in all of three colon cancer patients who received this treatment [28]. There is no doubt that high-avidity therapeutic TCR-T cells without an increased incidence of treatment-related on-and off-target AEs is desirable to achieve a better anticancer effect [70]. It is anticipated that this dilemma would be overcome by selection of appropriate target antigens, for example CTA, as has been demonstrated successfully for T cells gene-modified using an affinity-enhanced TCR targeting NY-ESO-1 [29]. Quite recently, however, it has been reported that T cells gene-modified using engineered TCRs with supra-physiologic affinities (Kd values <nM) specific for MAGE-A3, one of the leading CTAs, have caused fatal damage to vital organs, including the central nervous system (CNS) [25] and heart [26]. It is interesting that CDR2 was genetically engineered in both TCRs. In the former study, the HLA-A*02:01-restricted MAGE-A3-specific TCR, having recognized MAGE-A3/9/12 family proteins, caused unexpected severe neurological toxicity due to cross-reactivity with an epitope on MAGE-A12 expressed in the white matter of the brain (on-target AE) [25]. On the other hand, the HLA-A*01-restricted MAGE-A3-specific TCR employed in the latter study caused fatal cardiac toxicity due to an unexpected cross-reaction with the epitope on the cardiac muscle protein titin, which happened to have an amino acid sequence analogous to the original target on MAGE-A3 (off-target AE) [26]. The most important lesson from these cases is that in the strict sense, a serious AE is not predictable, even if the chosen antigen is appropriate and intensive preclinical studies have been performed. Accordingly, the authors of the latter report have proposed a novel screening system using a relevant HLA-expressing vital organ cell panel generated from induced pluripotent stem cells (iPSCs) to prospectively detect any risk of serious self-reactivity mediated by T cells gene-modified to express TCRs with supra-physiologic affinity.
Anti-CD19 CAR-T cell therapy, currently building on its success, also has problems to be solved. Normal B-cell aplasia in patients treated with anti-CD19-CAR-T cells represents another on-target AE that has so far been manageable using immunoglobulin replacement [51,55], although this problem still remains an unaddressed in the long term, and may have an economic impact on healthcare. Once a B-cell malignancy has been eradicated, any remaining anti-CD19-CAR-T cells should be ablated in order to facilitate normal B-cell reconstitution. A fatal on-target AE has also been observed during CAR-T-cell therapy. This was a case of serious pulmonary failure ending in multi-organ failure due to a massive release of cytokine in a patient with metastatic colon cancer who received CAR-T cells specific to human epithelial growth factor 2 (Her2, CD340) in combination with lymphodepletion [65]. This Her2-specific CAR construct comprised CD28, 4-1BB and CD3ζ in the cytoplasmic signaling domain, i.e., a “souped-up” 3rd-generation CAR construct. Although at a low level, Her2 was broadly expressed in pulmonary epithelial cells, and this was assumed to have caused the severe pulmonary injury as an on-target AE.
A braking system by which T cells are inherently equipped for excessive activation via TCR signaling, i.e., activation-induced cell death (AICD), might also prevent overproduction of TCR-T cells [71]. On the other hand, because CAR-T cells can non-physiologically overcome barriers to proliferation and persistence, a serious off-target AE directed against normal tissues may be characterized by massive release of pro-inflammatory cytokines in vivo from long-lasting hyperactive CAR-T cells, i.e., cytokine release syndrome (CRS). For example, transient cardiac dysfunction observed in patients who had received anti-CD19-CAR-T cells was not due to CD19 expression in heart tissue, and elevated serum levels of IFN-γ, TNF-α and IL-6 were observed [51,53]. In a recent clinical trial using anti-CD19-CAR-T cells, seven of 16 patients with chemotherapy-resistant B-ALL developed CRS including three cases of serious consciousness disturbance requiring ventilator support [56]. In the same report, the authors also highlighted the efficacy of an anti-IL-6 receptor mAb (tosilizumab) for treatment of severe CRS and the value of CRP for predicting its incidence [72]. Another off-target AE is tumor lysis syndrome (TLS) due to rapid and massive destruction of tumor cells, being characterized pathophysiologically by extremely elevated levels of serum phosphate, potassium and uric acid. These symptoms considerably overlap those of CRS [72]. In the clinical trial described above, the severity of CRS was positively correlated with the extent of the residual tumor cell burden upon infusion of anti-CD19-CAR-T cells [56]. Such CRS upon infusion of CAR-T cells was finally managed successfully by rearrangement of the CAR-T cell transfusion schedule, thus avoiding the risk of TLS [73]. Another occasionally life-threatening off-target AE is macrophage activation syndrome (MAS), characterized by systemic inflammatory symptoms and pancytopenia due to hemophagocytosis in the bone marrow, although its precise mechanism is still undetermined [56]. Another severe transient cardiac dysfunction as an off-target AE was experienced in a clinical trial using CAR-T cells specific for mesothelin. This event was due to anaphylaxis mediated by formation of a human anti-mouse antibody against the murine component of the CAR construct [74].

5. Attempts to Address Challenging Issues

The improved clinical outcomes resulting from the development of lymphodepletion [3,4] and the use of anti-CD19-CAR-T cells for B-cell malignancies [51,52,53,54,55,56,59,60,61] suggest that many of the problems associated with gene-modified T-cell-based anticancer adoptive immunotherapy have been solved. However, a number of unsolved issues remain to be addressed before this treatment option can be widely accepted in a clinical setting (Figure 3).
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Figure 3. Unsolved issues for the establishment of successful antileukemia adoptive immunotherapy using gene-modified T cells.
Figure 3. Unsolved issues for the establishment of successful antileukemia adoptive immunotherapy using gene-modified T cells.
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For the establishment of successful antileukemia adoptive immunotherapy using gene-modified T cells, various factors still remain unresolved in both the in vitro phase of T-cell manipulation (right) and the in vivo phase of clinical procedures (left). These factors mutually impact each other and eventually dictate the clinical outcome. Factors involved in the in vitro phase of T-cell manipulation will impact on the quality and quantity of the gene-modified T-cell product. On the other hand, factors associated with the clinical procedures would include how and when the T cells are harvested, how the gene-modified T cells are infused, i.e., with or without lymphodepletion, how unwanted on- and off-target AEs can be avoided, how the gene-modified T cells accumulate in local tumor tissues, and how the infused T cells are able to remain durably viable and active thereafter in vivo. At the same time, it is also important to overcome the immunosuppressive tumor microenvironment in patients with leukemia. Details are discussed in the Attempts to address challenging issues section. Ag: antigen, DC: dendritic cells, APC: antigen-presenting cells, TCR-T : relevant TCR gene-modified T cells, CAR-T : relevant CAR gene-modified T cells, AE: adverse effect.
Above all, in order to avoid serious on-target AEs, the choice of an appropriate antigen is indisputably important. The ideal target antigen should be selectively expressed by cancer cells, but absent in normal tissues, and sufficiently immunogenic to generate T-cell responses, resulting in effective anticancer reactivity without damage to normal cells. Strictly, two types of antigen meet these requirements: (i) viral antigens in virus-induced tumors, and (ii) mutated antigens encoded by causative gene alterations in cancer cells. It is anticipated that CAR-T cells or TCR-T cells, which are harnessed to the defined viral epitopes, would be able to eliminate virus-transformed cancers. In addition, whole-exon sequencing of cancer cells has recently identified novel candidate mutations in melanoma [75]. In the context of leukemia, identification of such mutations in a leukemia stem cell may open a new door to immunotherapy in the near future [76].
In order to establish the more powerful gene-modified T cells against cancer, TCR-T cells bearing supra-physiologic affinities and CAR-T cells displaying unnaturally enhanced proliferation and persistence upon target recognition have been developed, and both resulting in overwhelming extension of functional range of naturally occurring T cells. Accordingly, serious on- or off-target AEs have inevitably emerged, and these AEs are difficult to predict using conventional methods. An iPS-derived vital-organ cell panel expressing common HLA haplotypes might provide a breakthrough in this respect [26].
On the other hand, a novel vector system that simultaneously encodes a suicide system has been developed in order to eliminate gene-modified T cells when they display unwanted toxicities mediated by infused gene-modified T cells, for example, thymidine kinase gene of herpes simplex virus [77]. Most recently, a caspase switch triggering apoptosis has shown clinical promise, allowing effective elimination of infused CAR-T cells and resolution of clinical symptoms in patients manifesting GVHD [78].
In addition to artificial receptor engineering, the functional features of T cells in the context of gene modification have also become highlighted. To achieve sufficient expansion and long-term persistence of infused gene-modified T cells following the contact with cancer cells in vivo, the advantages of naïve or central memory T cells for TCR or CAR gene modification have already been demonstrated [79,80]. From this viewpoint, some excellent artificial antigen-presenting cells capable of expanding gene-modified T cells retaining certain desirable phenotypes have been developed. Cell-based artificial APCs using K562 are clinically available. K562, a human erythroleukemia cell line inherently lacks almost HLA molecules which enables to minimize the allo-responsive/ non-target-specific T cell proliferation, and well tolerates gene-modification which enables to express desirable HLA class I molecule for CD8 T cell and HLA class II for CD4 T in the setting of epitope-specific T cell expansion, and to express co-stimulatory molecules including CD80, CD83, 4.1BB-L for further T cell expansion [81]. Accordingly, the use of suitable T-cell subsets for gene modification might obviate the need for chemo-radiotherapeutic lymphodepleting preconditioning, which is substantially associated with treatment-related toxicity.
Finally, the combined use of immunological checkpoint blockade, for example employing anti-cytotoxic T-lymphocyte-associated antigen (CTLA)-4 antibody [82] and anti-programmed cell death (PD)-1/PD-ligand 1 (PD-L1) antibody [83,84], which have recently yielded promising results, especially in patients with solid tumors, appears to be a promising option. In a clinical trial using anti-melanoma T cells gene-modified to express a high-avidity murine gp100-specific TCR, the infused T cells became hypofunctional for the cognate epitope via the PD-1/ PD-L1 pathway a couple of months after infusion [85], as is the case for latent retrovirus infection [86]. Similar hypofunction mediated by infused CAR-gene-modified T cells targeting mesothelin for treatment of solid tumors has also been demonstrated in a mouse model [87].
Collectively, the selection of appropriate T-cell subsets for gene modification and the blockade of immunological checkpoints should be able to improve not only the life-span, but also the functional persistence of therapeutically infused gene-modified T cells in vivo, resulting in better clinical outcomes.

6. Conlusions

In the context of antileukemia adoptive immunotherapy using T cells gene-modified to express leukemia antigen-specific receptors aimed at the cure of leukemia, the final goal is to induce durable protective immunity against disease progression in patients without collateral damage to normal tissues, and so far this has been achieved only for successful cases of allo-HSCT. For this purpose, various sophisticated approaches are currently being examined, and in the near future this treatment option might be able to take the place of allo-HSCT for patients with certain types of leukemia, especially those who are ineligible for allo-HSCT or for whom timely acquisition of a suitable donor is not possible.

Conflict of Interest

The author has no conflicts of interest to declare.

References

  1. Miller, J.S.; Warren, E.H.; van den Brink, M.R.; Ritz, J.; Shlomchik, W.D.; Murphy, W.J.; Barrett, A.J.; Kolb, H.J.; Giralt, S.; Bishop, M.R.; et al. NCI First International Workshop on The Biology, Prevention, and Treatment of Relapse After Allogeneic Hematopoietic Stem Cell Transplantation: Report from the Committee on the Biology Underlying Recurrence of Malignant Disease following Allogeneic HSCT: Graft-versus-Tumor/Leukemia Reaction. Biol. Blood Marrow Transplant. 2010, 16, 565–586. [Google Scholar]
  2. Blyth, E.; Clancy, L.; Simms, R.; Ma, C.K.; Burgess, J.; Deo, S.; Byth, K.; Dubosq, M.C.; Shaw, P.J.; Michlethwaite, K.P.; et al. Donor-derived CMV-specific T cells reduce the requirement for CMV-directed pharmacotherapy after allogeneic stem cell transplantation. Blood 2013, 121, 3745–3758. [Google Scholar] [CrossRef] [PubMed]
  3. Rosenberg, S.A.; Dudley, M.E. Adoptive cell therapy for the treatment of patients with metastatic melanoma. Curr. Opin. Immunol. 2009, 21, 233–240. [Google Scholar] [CrossRef] [PubMed]
  4. Dudley, M.E.; Yang, J.C.; Sherry, R.; Hughes, M.S.; Royal, R.; Kammula, U.; Robbins, P.F.; Huang, J.; Citrin, D.E.; Leitman, S.F.; et al. Adoptive cell therapy for patients with metastatic melanoma: Evaluation of intensive myeloablative chemoradiation preparative regimens. J. Clin. Oncol. 2008, 26, 5233–5239. [Google Scholar] [CrossRef] [PubMed]
  5. Kerkar, S.P.; Muranski, P.; Kaiser, A.; Boni, A.; Sanchez-Perez, L.; Yu, Z.; Palmer, D.C.; Reger, R.N.; Borman, Z.A.; Zhang, L.; Morgan, R.A.; et al. Tumor-specific CD8+ T cells expressing interleukin-12 eradicate established cancers in lymphodepleted hosts. Cancer Res. 2010, 70, 6725–6734. [Google Scholar] [CrossRef] [PubMed]
  6. Muranski, P.; Boni, A.; Wrzesinski, C.; Citrin, D.E.; Rosenberg, S.A.; Childs, R.; Restifo, N.P. Increased intensity lymphodepletion and adoptive immunotherapy—How far can we go? Nat. Clin. Pract. Oncol. 2006, 3, 668–681. [Google Scholar] [CrossRef] [PubMed]
  7. Miller, J.S.; Soignier, Y.; Panoskaltsis-Mortari, A.; McNearney, S.A.; Yun, G.H.; Fautsch, S.K.; McKenna, D.; Le, C.; Defor, T.E.; Burns, L.J.; et al. Successful adoptive transfer and in vivo expansion of human haploidentical NK cells in patients with cancer. Blood 2005, 105, 3051–3057. [Google Scholar] [CrossRef] [PubMed]
  8. Ferrara, J.L.; Levine, J.E.; Reddy, P.; Holler, E. Graft-versus-host disease. Lancet 2009, 373, 1550–1561. [Google Scholar] [CrossRef] [PubMed]
  9. Besser, M.J.; Shapira-Frommer, R.; Treves, A.J.; Zippel, D.; Itzhaki, O.; Schallmach, E.; Kubi, A.; Shalmon, B.; Hardan, I.; Catane, R.; et al. Minimally cultured or selected autologous tumor-infiltrating lymphocytes after a lympho-depleting chemotherapy regimen in metastatic melanoma patients. J. Immunother. 2009, 32, 415–423. [Google Scholar] [PubMed]
  10. Vincent, K.; Roy, D.C.; Perreault, C. Next-generation leukemia immunotherapy. Blood 2011, 118, 2951–2959. [Google Scholar] [CrossRef] [PubMed]
  11. Jamieson, B.D.; Ahmed, R. T cell memory. Long-term persistence of virus-specific cytotoxic T cells. J. Exp. Med. 1989, 169, 1993–2005. [Google Scholar] [CrossRef] [PubMed]
  12. Michie, C.A.; McLean, A.; Alcock, C.; Beverly, P.C. Lifespan of human lymphocyte subsets defined by CD45 isoform. Nature 1992, 360, 264–265. [Google Scholar] [CrossRef] [PubMed]
  13. Koya, R.C.; Mok, S.; Comin-Anduix, B.; Chodon, T.; Radu, C.G.; Nishimura, M.I.; Witte, O.N.; Ribas, A. Kinetic phases of distribution and tumor targeting by T cell receptor engineered lymphocytes inducing robust antitumor responses. Proc. Natl. Acad. Sci. USA 2010, 107, 14286–14291. [Google Scholar] [CrossRef] [PubMed]
  14. Hildebrandt, M.; Peggs, K.; Uharek, L.; Bollard, C.M.; Heslop, H.E. Immunotherapy: Opportunities, risks and future perspectives. Cytotherapy 2014, 16, S120–S129. [Google Scholar] [CrossRef] [PubMed]
  15. Park, T.S.; Rosenberg, S.A.; Morgan, R.A. Treating cancer with genetically engineered T cells. Trends Biotechnol. 2011, 29, 550–557. [Google Scholar] [CrossRef] [PubMed]
  16. Ochi, T.; Fujiwara, H.; Yasukawa, M. Requisite considerations for successful adoptive immunotherapy with engineered T-lymphocytes using tumor antigen-specific T-cell receptor gene transfer. Expert Opin. Biol. Ther. 2011, 11, 699–713. [Google Scholar] [CrossRef] [PubMed]
  17. Au, P.; Hursh, D.A.; Lim, A.; Moos, M.C. Jr.; Oh, S.S.; Schneider, B.S.; Witten, C.M. FDA oversight of cell therapy clinical trials. Sci. Transl. Med. 2012, 4, 49fs31. [Google Scholar] [CrossRef]
  18. Fujiwara, H. Adoptive T-cell therapy for hematological malignancies using T cells gene-modified to express tumor antigen-specific receptors. Int. J. Hematol. 2014, 99, 123–131. [Google Scholar] [CrossRef] [PubMed]
  19. Corrigan-Curay, J.; Kiem, H.P.; Baltimore, D.; O'Reilly, M.; Brentjens, R.J.; Cooper, L.; Forman, S.; Gottschalk, S.; Greenberg, P.; Junghans, R.; et al. T-cell immunotherapy: Looking forward. Mol. Ther. 2014, 22, 1564–1574. [Google Scholar] [CrossRef] [PubMed]
  20. June, C.H. Principles of adoptive T cell cancer therapy. J. Clin. Inv. 2007, 117, 1204–1212. [Google Scholar] [CrossRef]
  21. Schmitt, T.M.; Aggen, D.H.; Stromnes, I.M.; Dossett, M.L.; Richman, S.A.; Kranz, D.M.; Greenberg, P.D. Enhanced-affinity murine T-cell receptors for tumor/self-antigens can be safe in gene therapy despite surpassing the threshold for thymic selection. Blood 2013, 122, 348–356. [Google Scholar] [CrossRef] [PubMed]
  22. Li, Y.; Moysey, R.; Molloy, P.E.; Vuidepot, A.L.; Mahon, T.; Baston, E.; Dunn, S.; Liddy, N.; Jacob, J.; Jakobsen, B.K.; et al. Directed evolution of human T-cell receptors with picomolar affinities by phage display. Nat. Biotechnol. 2005, 23, 349–354. [Google Scholar] [CrossRef] [PubMed]
  23. Stauss, H.J. Immunotherapy with CTLs restricted by nonself MHC. Immunol. Today 1999, 20, 180–183. [Google Scholar] [CrossRef] [PubMed]
  24. Stanislawski, T.; Voss, R.H.; Lotz, C.; Sadovnikova, E.; Willemsen, R.A.; Kuball, J.; Ruppert, T.; Bolhuis, R.L.; Melief, C.J.; Huber, C.; et al. Circumventing tolerance to a human MDM2-derived tumor antigen by TCR gene transfer. Nat. Immunol. 2001, 2, 962–970. [Google Scholar] [CrossRef] [PubMed]
  25. Morgan, R.A.; Chinnasamy, N.; Abate-Daga, D.; Gros, A.; Robbins, P.F.; Zheng, Z.; Dudley, M.E.; Feldman, S.A.; Yang, J.C.; Sherry, R.M.; et al. Cancer regression and neurological toxicity following anti-MAGE-A3 TCR gene therapy. J. Immunother. 2013, 36, 133–151. [Google Scholar] [CrossRef] [PubMed]
  26. Linette, G.P.; Stadtmauer, E.A.; Maus, M.V.; Rapoport, A.P.; Levine, B.L.; Emery, L.; Litzky, L.; Bagg, A.; Carreno, B.M.; Cimino, P.J.; et al. Cardiovascular toxicity and titin cross-reactivity of affinity-enhanced T cells in myeloma and melanoma. Blood 2013, 122, 863–871. [Google Scholar] [CrossRef] [PubMed]
  27. Johnson, L.A.; Morgan, R.A.; Dudley, M.E.; Cassard, L.; Yang, J.C.; Hughes, M.S.; Kammula, U.S.; Royal, R.E.; Sherry, R.M.; Wunderlich, J.R.; et al. Gene therapy with human and mouse T-cell receptors mediates cancer regression and targets normal tissues expressing cognate antigen. Blood 2009, 114, 535–546. [Google Scholar] [CrossRef] [PubMed]
  28. Parkhurst, M.R.; Yang, J.C.; Langan, R.C.; Dudley, M.E.; Nathan, D.A.; Feldman, S.A.; Davis, J.L.; Morgan, R.A.; Merino, M.J.; Sherry, M.R.; et al. T cells targeting carcinoembryonic antigen can mediate regression of metastatic colorectal cancer but induce severe transient colitis. Mol. Ther. 2011, 19, 620–626. [Google Scholar] [CrossRef] [PubMed]
  29. Robbins, P.F.; Morgan, R.A.; Feldman, S.A.; Yang, J.C.; Sherry, R.M.; Dudley, M.E.; Wunderlich, J.R.; Nahvi, A.V.; Helman, L.J.; Mackall, C.L.; et al. Tumor regression in patients with metastatic synovial cell sarcoma and melanoma using genetically engineered lymphocytes reactive with NY-ESO-1. J. Clin. Oncol. 2011, 29, 917–924. [Google Scholar] [CrossRef] [PubMed]
  30. Cohen, C.J.; Li, Y.F.; El-Gamil, M.; Robbins, P.F.; Rosenberg, S.A.; Morgan, R.A. Enhanced antitumor activity of T cells engineered to express T-cell receptors with a second disulfide bond. Cancer Res. 2007, 67, 3898–3903. [Google Scholar] [CrossRef] [PubMed]
  31. Thomas, S.; Xue, S.A.; Cesco-Gaspere, M.; San José, E.; Hart, D.P.; Wong, V.; Debets, R.; Alarcon, B.; Morris, E.; Stauss, H.J. Targeting the Wilms tumor antigen 1 by TCR gene transfer: TCR variants improve tetramer binding but not the function of gene modified human T cells. J. Immunol. 2007, 179, 5803–5810. [Google Scholar] [CrossRef] [PubMed]
  32. Bendle, G.M.; Linnemann, C.; Hooijkaas, A.I.; Bies, L.; de Witte, M.A.; Jorritsma, A.; Kaiser, A.D.; Pouw, N.; Debets, R.; Kieback, E.; et al. Lethal graft-versus-host disease in mouse models of T cell receptor gene therapy. Nat. Med. 2010, 16, 565–570. [Google Scholar] [CrossRef] [PubMed]
  33. Okamoto, S.; Mineno, J.; Ikeda, H.; Fujiwara, H.; Yasukawa, M.; Shiku, H.; Kato, I. Improved expression and reactivity of transduced tumor-specific TCRs in human lymphocytes by specific silencing of endogenous TCR. Cancer Res. 2009, 69, 9003–9011. [Google Scholar] [CrossRef] [PubMed]
  34. Okamoto, S.; Amaishi, Y.; Goto, Y.; Ikeda, H.; Fujiwara, H.; Kuzushima, K.; Yasukawa, M.; Shiku, H.; Mineno, J. A Promising Vector for TCR Gene Therapy: Differential Effect of siRNA, 2A Peptide, and Disulfide Bond on the Introduced TCR Expression. Mol. Ther. Nucleic Acids 2012, 1, e63. [Google Scholar] [CrossRef] [PubMed]
  35. Provasi, E.; Genovese, P.; Lombardo, A.; Magnani, Z.; Liu, P.Q.; Reik, A.; Chu, V.; Paschon, D.E.; Zhang, L.; Kuball, J.; et al. Editing T cell specificity towards leukemia by zinc finger nucleases and lentiviral gene transfer. Nat Med. 2012, 18, 807–815. [Google Scholar] [CrossRef] [PubMed]
  36. Torikai, H.; Reik, A.; Liu, P.Q.; Zhou, Y.; Zhang, L.; Maiti, S.; Huls, H.; Miller, J.C.; Kebriaei, P.; Rabinovitch, B.; et al. A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR. Blood 2012, 119, 5697–5705. [Google Scholar] [CrossRef] [PubMed]
  37. Morgan, R.A.; Dudley, M.E.; Wunderlich, J.R.; Hughes, M.S.; Yang, J.C.; Sherry, R.M.; Royal, R.E.; Topalian, S.L.; Kammula, U.S.; Restifo, N.P.; et al. Cancer regression in patients after transfer of genetically engineered lymphocytes. Science 2006, 314, 126–129. [Google Scholar] [CrossRef]
  38. Davis, J.L.; Theoret, M.R.; Zheng, Z.; Lamers, C.H.; Rosenberg, S.A.; Morgan, R.A. Development of human anti-murine T-cell receptor antibodies in both responding and nonresponding patients enrolled in TCR gene therapy trials. Clin. Cancer Res. 2010, 16, 5852–5861. [Google Scholar] [CrossRef] [PubMed]
  39. Ochi, T.; Fujiwara, H.; Okamoto, S.; An, J.; Nagai, K.; Shirakata, T.; Mineno, J.; Kuzushima, K.; Shiku, H.; Yasukawa, M. Novel adoptive T-cell immunotherapy using a WT1-specific TCR vector encoding silencers for endogenous TCRs shows marked antileukemia reactivity and safety. Blood 2011, 118, 1495–1503. [Google Scholar] [CrossRef] [PubMed]
  40. Tebas, P.; Stein, D.; Tang, W.W.; Frank, I.; Wang, S.Q.; Lee, G.; Spratt, S.K.; Surosky, R.T.; Giedlin, M.A.; Nichol, G.; et al. Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N. Engl. J. Med. 2014, 370, 901–910. [Google Scholar] [CrossRef] [PubMed]
  41. Sadelain, M.; Brentjens, R.; Rivière, I. The basic principles of chimeric antigen receptor design. Cancer Discov. 2013, 3, 388–398. [Google Scholar] [CrossRef] [PubMed]
  42. Irving, B.A.; Weiss, A. The cytoplasmic domain of the T cell receptor zeta chain is sufficient to couple to receptor-associated signal transduction pathways. Cell 1991, 64, 891–901. [Google Scholar] [CrossRef] [PubMed]
  43. Eshhar, Z.; Waks, T.; Gross, G.; Schindler, D.G. Specific activation and targeting of cytotoxic lymphocytes through chimeric single chains consisting of antibody-binding domains and the gamma or zeta subunits of the immunoglobulin and T-cell receptors. Proc. Natl. Acad. Sci. USA 1993, 90, 720–724. [Google Scholar] [CrossRef] [PubMed]
  44. Gong, M.C.; Latouche, J.B.; Krause, A.; Heston, W.D.; Bander, N.H.; Sadelain, M. Cancer patient T cells genetically targeted to prostate-specific membrane antigen specifically lyse prostate cancer cells and release cytokines in response to prostate-specific membrane antigen. Neoplasia 1999, 1, 123–127. [Google Scholar] [CrossRef] [PubMed]
  45. Haynes, N.M.; Trapani, J.A.; Teng, M.W.; Jackson, J.T.; Cerruti, L.; Jane, S.M.; Kershaw, M.H.; Smyth, M.J.; Darcy, P.K. Rejection of syngeneic colon carcinoma by CTLs expressing single-chain antibody receptors codelivering CD28 costimulation. J. Immunol. 2002, 169, 5780–5786. [Google Scholar] [CrossRef] [PubMed]
  46. Carpenito, C.; Milone, M.C.; Hassan, R.; Simonet, J.C.; Lakhal, M.; Suhoski, M.M.; Varela-Rohena, A.; Haines, K.M.; Heitjan, D.F.; Albelda, S.M.; et al. Control of large, established tumor xenografts with genetically retargeted human T cells containing CD28 and CD137 domains. Proc. Natl.Acad. Sci. USA 2009, 106, 3360–3365. [Google Scholar] [PubMed]
  47. Hombach, A.A.; Abken, H. Costimulation by chimeric antigen receptors revisited the T cell antitumor response benefits from combined CD28-OX40 signalling. Int. J. Cancer. 2011, 129, 2935–2944. [Google Scholar] [PubMed]
  48. Finney, H.M.; Akbar, A.N.; Lawson, A.D. Activation of resting human primary T cells with chimeric receptors: Costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain. J. Immunol. 2004, 172, 104–113. [Google Scholar] [CrossRef] [PubMed]
  49. Finney, H.M.; Lawson, A.D.; Bebbington, C.R.; Weir, A.N. Chimeric receptors providing both primary and costimulatory signaling in T cells from a single gene product. J. Immunol. 1998, 161, 2791–2797. [Google Scholar] [PubMed]
  50. Hombach, A.; Wieczarkowiecz, A.; Marquardt, T.; Heuser, C.; Usai, L.; Pohl, C.; Seliger, B.; Abken, H. Tumor-specific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient IL-2 secretion and can be integrated into one combined CD28/CD3 zeta signaling receptor molecule. J. Immunol. 2001, 167, 6123–6131. [Google Scholar] [CrossRef] [PubMed]
  51. Kalos, M.; Levine, B.L.; Porter, D.L.; Katz, S.; Grupp, S.A.; Bagg, A.; June, C.H. T cells with chimeric antigen receptors have potent antitumor effects and can establish memory in patients with advanced leukemia. Sci. Transl. Med. 2011, 3, 95ra73. [Google Scholar] [CrossRef] [PubMed]
  52. Porter, D.L.; Levine, B.L.; Kalos, M.; Bagg, A.; June, C.H. Chimeric antigen receptor-modified T cells in chronic lymphoid leukemia. N. Engl. J. Med. 2011, 365, 725–733. [Google Scholar] [CrossRef] [PubMed]
  53. Kochenderfer, J.N.; Wilson, W.H.; Janik, J.E.; Dudley, M.E.; Stetler-Stevenson, M.; Feldman, S.A.; Maric, I.; Raffeld, M.; Nathan, D.A.; Lanier, B.J.; et al. Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19. Blood 2010, 116, 4099–4102. [Google Scholar] [PubMed]
  54. Brentjens, R.J.; Davila, M.L.; Riviere, I.; Park, J.; Wang, X.; Cowell, L.G.; Bartido, S.; Stefanski, J.; Taylor, C.; Olszewska, M.; et al. CD19-targeted T cells rapidly induce molecular remissions in adults with chemotherapy-refractory acute lymphoblastic leukemia. Sci. Transl. Med. 2013, 5, 177ra38. [Google Scholar] [CrossRef] [PubMed]
  55. Jensen, M.C.; Popplewell, L.; Cooper, L.J.; DiGiusto, D.; Kalos, M.; Ostberg, J.R.; Forman, S.J. Antitransgene rejection responses contribute to attenuated persistence of adoptively transferred CD20/CD19-specific chimeric antigen receptor redirected T cells in humans. Biol. Blood Marrow Transplant. 2010, 16, 1245–1256. [Google Scholar] [CrossRef] [PubMed]
  56. Davila, M.L.; Riviere, I.; Wang, X.; Bartido, S.; Park, J.; Curran, K.; Chung, S.S.; Stefanski, J.; Borquez-Ojeda, O.; Olszewska, M.; et al. Efficacy and toxicity management of 19–28z CAR T cell therapy in B cell acute lymphoblastic leukemia. Sci. Transl. Med. 2014, 6, 224ra25. [Google Scholar] [CrossRef] [PubMed]
  57. Gilham, D.E.; Debets, R.; Pule, M.; Hawkins, R.E.; Abken, H. T cells and solid tumors: Tuning T cells to challenge an inveterate foe. Trends Mol. Med. 2012, 18, 377–384. [Google Scholar] [CrossRef] [PubMed]
  58. Davila, M.L.; Brentjens, R.; Wang, X.; Rivière, I.; Sadelain, M. How do CARs work? : Early insights from recent clinical studies targeting CD19. Oncoimmunology 2012, 1, 1577–1583. [Google Scholar] [CrossRef] [PubMed]
  59. Kochenderfer, J.N.; Dudley, M.E.; Feldman, S.A.; Wilson, W.H.; Spaner, D.E.; Maric, I.; Stetler-Stevenson, M.; Phan, G.Q.; Hughes, M.S.; Sherry, R.M.; et al. B-cell depletion and remissions of malignancy along with cytokine-associated toxicity in a clinical trial of anti-CD19 chimeric-antigen-receptor-transduced T cells. Blood 2012, 119, 2709–2720. [Google Scholar] [CrossRef] [PubMed]
  60. Savoldo, B.; Ramos, C.A.; Liu, E.; Mims, M.P.; Keating, M.J.; Carrum, G.; Kamble, R.T.; Bollard, C.M.; Gee, A.P.; Mei, Z.; et al. CD28 costimulation improves expansion and persistence of chimeric antigen receptor-modified T cells in lymphoma patients. J. Clin. Invest. 2011, 121, 1822–1826. [Google Scholar] [CrossRef] [PubMed]
  61. Grupp, S.A.; Kalos, M.; Barrett, D.; Aplenc, R.; Porter, D.L.; Rheingold, S.R.; Teachey, D.T.; Chew, A.; Hauck, B.; Wright, J.F.; et al. Chimeric antigen receptor-modified T cells for acute lymphoid leukemia. N. Engl. J. Med. 2013, 368, 1509–1518. [Google Scholar] [PubMed]
  62. Chmielewski, M.; Hombach, A.; Heuser, C.; Adams, G.P.; Abken, H. T cell activation by antibody-like immunoreceptors: Increase in affinity of the single-chain fragment domain above threshold does not increase T cell activation against antigen-positive target cells but decreases selectivity. J. Immunol. 2004, 173, 7647–7653. [Google Scholar] [CrossRef] [PubMed]
  63. Long, A.H.; Haso, W.M.; Orentas, R.J. Lessons learned from a highly-active CD22-specific chimeric antigen receptor. Oncoimmunology 2013, 2, e23621. [Google Scholar] [CrossRef] [PubMed]
  64. Park, J.R.; Digiusto, D.L.; Slovak, M.; Wright, C.; Naranjo, A.; Wagner, J.; Meechoovet, H.B.; Bautista, C.; Chang, W.C.; Ostberg, J.R.; et al. Adoptive transfer of chimeric antigen receptor re-directed cytolytic T lymphocyte clones in patients with neuroblastoma. Mol. Ther. 2007, 15, 825–833. [Google Scholar] [PubMed]
  65. Morgan, R.A.; Yang, J.C.; Kitano, M.; Dudley, M.E.; Laurencot, C.M.; Rosenberg, S.A. Case report of a serious adverse event following the administration of T cells transduced with a chimeric antigen receptor recognizing ERBB2. Mol. Ther. 2010, 18, 843–851. [Google Scholar] [CrossRef] [PubMed]
  66. Louis, C.U.; Savoldo, B.; Dotti, G.; Pule, M.; Yvon, E.; Myers, G.D.; Rossig, C.; Russell, H.V.; Diouf, O.; Liu, E.; et al. Antitumor activity and long-term fate of chimeric antigen receptor-positive T cells in patients with neuroblastoma. Blood 2011, 118, 6050–6056. [Google Scholar] [CrossRef] [PubMed]
  67. Till, B.G.; Jensen, M.C.; Wang, J.; Qian, X.; Gopal, A.K.; Maloney, D.G.; Lindgren, C.G.; Lin, Y.; Pagel, J.M.; Budde, L.E.; et al. CD20-specific adoptive immunotherapy for lymphoma using a chimeric antigen receptor with both CD28 and 4–1BB domains: Pilot clinical trial results. Blood 2012, 119, 3940–3950. [Google Scholar] [CrossRef] [PubMed]
  68. Cheadle, E.J.; Sheard, V.; Rothwell, D.G.; Mansoor, A.W.; Hawkins, R.E.; Gilham, D.E. Differential role of Th1 and Th2 cytokines in autotoxicity driven by CD19-specific second-generation chimeric antigen receptor T cells in a mouse model. J. Immunol. 2014, 192, 3654–3665. [Google Scholar] [CrossRef] [PubMed]
  69. Chapuis, A.G.; Ragnarsson, G.B.; Nguyen, H.N.; Chaney, C.N.; Pufnock, J.S.; Schmitt, T.M.; Duerkopp, N.; Roberts, I.M.; Pogosov, G.L.; Ho, W.Y.; et al. Transferred WT1-reactive CD8+ T cells can mediate antileukemic activity and persist in post-transplant patients. Sci. Transl. Med. 2013, 5, 174ra27. [Google Scholar] [PubMed]
  70. Valitutti, S.; Lanzavecchia, A. Serial triggering of TCRs: A basis for the sensitivity and specificity of antigen recognition. Immunol. Today. 1997, 18, 299–304. [Google Scholar] [CrossRef] [PubMed]
  71. Alexander-Miller, M.A.; Leggatt, G.R.; Berzofsky, J.A. Selective expansion of high- or low-avidity cytotoxic T lymphocytes and efficacy for adoptive immunotherapy. Proc. Natl. Acad. Sci. USA 1996, 93, 4102–4107. [Google Scholar] [CrossRef] [PubMed]
  72. Nakamura, M.; Oda, S.; Sadahiro, T.; Hirayama, Y.; Tateishi, Y.; Abe, R.; Hirasawa, H. The role of hypercytokinemia in the pathophysiology of tumor lysis syndrome (TLS) and the treatment with continuous hemodiafiltration using a polymethylmethacrylate membrane hemofilter (PMMA-CHDF). Transfus. Apher. Sci. 2009, 40, 41–47. [Google Scholar] [CrossRef] [PubMed]
  73. Brentjens, R.; Yeh, R.; Bernal, Y.; Riviere, I.; Sadelain, M. Treatment of chronic lymphocytic leukemia with genetically targeted autologous T cells: Case report of an unforeseen adverse event in a phase I clinical trial. Mol. Ther. 2010, 18, 666–668. [Google Scholar] [CrossRef] [PubMed]
  74. Maus, M.V.; Haas, A.R.; Beatty, G.L.; Albelda, S.M.; Levine, B.L.; Liu, X.; Zhao, Y.; Kalos, M.; June, C.H. T cells expressing chimeric antigen receptors can cause anaphylaxis in humans. Cancer Immunol. Res. 2013, 1, 26–31. [Google Scholar] [CrossRef] [PubMed]
  75. Robbins, P.F.; Lu, Y.C.; El-Gamil, M.; Li, Y.F.; Gross, C.; Gartner, J.; Lin, J.C.; Teer, J.K.; Cliften, P.; Tycksen, E.; et al. Mining exomic sequencing data to identify mutated antigens recognized by adoptively transferred tumor-reactive T cells. Nat. Med. 2013, 19, 747–752. [Google Scholar] [CrossRef] [PubMed]
  76. Snauwaert, S.; Vandekerckhove, B.; Kerre, T. Can immunotherapy specifically target acute myeloid leukemic stem cells? Oncoimmunology 2013, 2, e22943. [Google Scholar] [CrossRef] [PubMed]
  77. Ciceri, F.; Bonini, C.; Stanghellini, M.T.; Bondanza, A.; Traversari, C.; Salomoni, M.; Turchetto, L.; Colombi, S.; Bernardi, M.; Peccatori, J.; et al. Infusion of suicide-gene-engineered donor lymphocytes after family haploidentical haemopoietic stem-cell transplantation for leukaemia (the TK007 trial): A non-randomised phase I-II study. Lancet Oncol. 2009, 10, 489–500. [Google Scholar] [CrossRef] [PubMed]
  78. Di Stasi, A.; Tey, S.K.; Dotti, G.; Fujita, Y.; Kennedy-Nasser, A.; Martinez, C.; Straathof, K.; Liu, E.; Durett, A.G.; Grilley, B.; et al. Inducible apoptosis as a safety switch for adoptive cell therapy. N. Engl. J. Med. 2011, 365, 1673–1683. [Google Scholar] [CrossRef] [PubMed]
  79. Berger, C.; Jensen, M.C.; Lansdorp, P.M.; Gough, M.; Elliott, C.; Riddell, S.R. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates. J. Clin. Invest. 2008, 118, 294–305. [Google Scholar] [CrossRef] [PubMed]
  80. Hinrichs, C.S.; Borman, Z.A.; Cassard, L.; Gattinoni, L.; Spolski, R.; Yu, Z.; Sanchez-Perez, L.; Muranski, P.; Kern, S.J.; Logun, C.; et al. Adoptively transferred effector cells derived from naive rather than central memory CD8+ T cells mediate superior antitumor immunity. Proc. Natl. Acad. Sci. USA 2009, 106, 17469–17474. [Google Scholar] [CrossRef] [PubMed]
  81. Butler, M.O.; Hirano, N. Human cell-based artificial antigen-presenting cells for cancer immunotherapy. Immunol. Rev. 2013, 257, 191–209. [Google Scholar] [CrossRef]
  82. Hodi, F.S.; O'Day, S.J.; McDermott, D.F.; Weber, R.W.; Sosman, J.A.; Haanen, J.B.; Gonzalez, R.; Robert, C.; Schadendorf, D.; Hassel, J.C.; et al. Improved survival with ipilimumab in patients with metastatic melanoma. N. Engl. J. Med. 2010, 363, 711–723. [Google Scholar] [CrossRef] [PubMed]
  83. Topalian, S.L.; Hodi, F.S.; Brahmer, J.R.; Gettinger, S.N.; Smith, D.C.; McDermott, D.F.; Powderly, J.D.; Carvajal, R.D.; Sosman, J.A.; Atkins, M.B.; et al. Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N. Engl. J. Med. 2012, 366, 2443–2454. [Google Scholar] [PubMed]
  84. Brahmer, J.R.; Tykodi, S.S.; Chow, L.Q.; Hwu, W.J.; Topalian, S.L.; Hwu, P.; Drake, C.G.; Camacho, L.H.; Kauh, J.; Odunsi, K.; et al. Safety and activity of anti-PD-L1 antibody in patients with advanced cancer. N. Engl. J. Med. 2012, 366, 2455–2465. [Google Scholar] [CrossRef] [PubMed]
  85. Abate-Daga, D.; Hanada, K.; Davis, J.L.; Yang, J.C.; Rosenberg, S.A.; Morgan, R.A. Expression profiling of TCR-engineered T cells demonstrates overexpression of multiple inhibitory receptors in persisting lymphocytes. Blood 2013, 122, 1399–1410. [Google Scholar]
  86. Day, C.L.; Kaufmann, D.E.; Kiepiela, P.; Brown, J.A.; Moodley, E.S.; Reddy, S.; Mackey, E.W.; Miller, J.D.; Leslie, A.J.; De Pierres, C.; et al. PD-1 expression on HIV-specific T cells is associated with T-cell exhaustion and disease progression. Nature 2006, 443, 350–354. [Google Scholar] [CrossRef] [PubMed]
  87. Moon, E.K.; Wang, L.C.; Dolfi, D.V.; Wilson, C.B.; Ranganathan, R.; Sun, J.; Kapoor, V.; Scholler, J.; Puré, E.; Milone, M.C.; et al. Multifactorial T-cell hypofunction that is reversible can limit the efficacy of chimeric antigen receptor-transduced human T cells in solid tumors. Clin. Cancer Res. 2014, 20, 4262–4273. [Google Scholar] [PubMed]

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MDPI and ACS Style

Fujiwara, H. Adoptive Immunotherapy for Hematological Malignancies Using T Cells Gene-Modified to Express Tumor Antigen-Specific Receptors. Pharmaceuticals 2014, 7, 1049-1068. https://doi.org/10.3390/ph7121049

AMA Style

Fujiwara H. Adoptive Immunotherapy for Hematological Malignancies Using T Cells Gene-Modified to Express Tumor Antigen-Specific Receptors. Pharmaceuticals. 2014; 7(12):1049-1068. https://doi.org/10.3390/ph7121049

Chicago/Turabian Style

Fujiwara, Hiroshi. 2014. "Adoptive Immunotherapy for Hematological Malignancies Using T Cells Gene-Modified to Express Tumor Antigen-Specific Receptors" Pharmaceuticals 7, no. 12: 1049-1068. https://doi.org/10.3390/ph7121049

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