Phorbol Ester Modulation of Ca2+ Channels Mediates Nociceptive Transmission in Dorsal Horn Neurones
Abstract
:1. Introduction
2. Experimental Section
2.1. Acute spinal Cord Slice Preparation and Solutions
2.2. Electrophysiological Recording
2.3. Pharmacology
3. Results and Discussion
3.1. Effect of PMA on Synaptic Transmission in Dorsal Horn Neurones
sPSC amplitude (pA) | sPSC frequency (Hz) | sPSC half-width (ms) | |
---|---|---|---|
WT (n = 6) | 43.5 ± 4.2 | 0.69 ± 0.16 | 6.85 ± 0.57 |
PMA (n = 6) | 41.8 ± 5.0 | 1.40 ± 0.36 * | 6.72 ± 0.47 |
CaV2.3(−/−) (n = 8) | 57.3 ± 9.7 | 1.00 ± 0.15 | 7.26 ± 0.42 |
PMA (n = 8) | 47.4 ± 5.3 | 1.08 ± 0.21 | 7.27 ± 0.40 |
3.2. Contribution of CaV2 VDCC Subtypes to PMA Effects on Synaptic Transmission in Dorsal Horn Neurones
4. Conclusions
Acknowledgements
Conflict of Interest
References
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Yang, L.; Topia, I.; Schneider, T.; Stephens, G.J. Phorbol Ester Modulation of Ca2+ Channels Mediates Nociceptive Transmission in Dorsal Horn Neurones. Pharmaceuticals 2013, 6, 777-787. https://doi.org/10.3390/ph6060777
Yang L, Topia I, Schneider T, Stephens GJ. Phorbol Ester Modulation of Ca2+ Channels Mediates Nociceptive Transmission in Dorsal Horn Neurones. Pharmaceuticals. 2013; 6(6):777-787. https://doi.org/10.3390/ph6060777
Chicago/Turabian StyleYang, Li, Iqbal Topia, Toni Schneider, and Gary J. Stephens. 2013. "Phorbol Ester Modulation of Ca2+ Channels Mediates Nociceptive Transmission in Dorsal Horn Neurones" Pharmaceuticals 6, no. 6: 777-787. https://doi.org/10.3390/ph6060777