Next Article in Journal / Special Issue
Disease-Causing Allele-Specific Silencing by RNA Interference
Previous Article in Journal
The Three Catecholics Benserazide, Catechol and Pyrogallol are GPR35 Agonists
Previous Article in Special Issue
Design of siRNA Therapeutics from the Molecular Scale
Open AccessReview

Delivery of RNAi-Based Oligonucleotides by Electropermeabilization

1
Centre National de la Recherche Scientifique (CNRS), Institut de Pharmacologie et de Biologie Structurale (IPBS) BP 64182, 205 route de Narbonne, Toulouse F-31077, France
2
Université Paul Sabatier de Toulouse, IPBS, Toulouse F-31077, France
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2013, 6(4), 510-521; https://doi.org/10.3390/ph6040510
Received: 31 December 2012 / Revised: 19 March 2013 / Accepted: 27 March 2013 / Published: 10 April 2013
(This article belongs to the Special Issue RNAi-Based Therapeutics)
For more than a decade, understanding of RNA interference (RNAi) has been a growing field of interest. The potent gene silencing ability that small oligonucleotides have offers new perspectives for cancer therapeutics. One of the present limits is that many biological barriers exist for their efficient delivery into target cells or tissues. Electropermeabilization (EP) is one of the physical methods successfully used to transfer small oligonucleotides into cells or tissues. EP consists in the direct application of calibrated electric pulses to cells or tissues that transiently permeabilize the plasma membranes, allowing efficient in vitro and in vivo. cytoplasmic delivery of exogenous molecules. The present review reports on the type of therapeutic RNAi-based oligonucleotides that can be electrotransferred, the mechanism(s) of their electrotransfer and the technical settings for pre-clinical purposes. View Full-Text
Keywords: electroporation; siRNA; miRNA; LNA; siLNA electroporation; siRNA; miRNA; LNA; siLNA
Show Figures

Figure 1

MDPI and ACS Style

Chabot, S.; Pelofy, S.; Teissié, J.; Golzio, M. Delivery of RNAi-Based Oligonucleotides by Electropermeabilization. Pharmaceuticals 2013, 6, 510-521.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop