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Volume 17
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10.3390/ph17030402
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Article

Chlojaponilactone B Attenuates THP-1 Macrophage Pyroptosis by Inhibiting the TLR/MyD88/NF-κB Pathway

by
Qiyin Wen
1,
Bingjinfeng Zhan
1,
Lu Jin
1,
Zijing Peng
1,
Ju Liu
1,
Longping Zhu
1,
Depo Yang
1,
Xinjun Xu
1,
Lixia Zhang
2,
Ge Li
2 and
Zhimin Zhao
1,*
1
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
2
Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2024, 17(3), 402; https://doi.org/10.3390/ph17030402
Submission received: 9 February 2024 / Revised: 12 March 2024 / Accepted: 18 March 2024 / Published: 21 March 2024
(This article belongs to the Section Natural Products)
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Abstract

Pyroptosis, an innate immune response, plays a crucial role in the pathological process of inflammatory diseases. Although pyroptosis blockade is considered a potential therapeutic strategy, no ideal candidate drug has been identified. The natural product Chojaponilactone B (CJB) has demonstrated anti-inflammatory effects, but its role in macrophage pyroptosis has not been studied. This study aimed to investigate the effect and mechanism of CJB in inhibiting macrophage pyroptosis. Using an LPS/ATP-induced THP-1 macrophage pyroptosis model, we found that CJB significantly inhibited pyroptosis and reduced the levels of NLRP3, caspase 1, N-GSDMD, and inflammatory cytokines IL-1β and IL-18. RNA sequencing analysis revealed that CJB interfered with LPS/ATP-induced THP-1 macrophage gene expression, suggesting involvement in anti-inflammatory and anti-pyroptotic signaling pathways. Additionally, CJB suppressed LPS/ATP-induced elevations in TLRs, MyD88, pro-IL-1β, and NF-κB and blocked NF-κB p65 nuclear translocation. In summary, CJB inhibits NLRP3 activation and macrophage pyroptosis through the TLR/MyD88/NF-κB pathway, providing important evidence for its development as a potential drug for treating pyroptosis-related inflammatory diseases.
Keywords: Chojaponilactone B; lindenane sesquiterpenoid lactone; NLRP3 inflammasome; pyroptosis; anti-inflammation; Chloranthus Chojaponilactone B; lindenane sesquiterpenoid lactone; NLRP3 inflammasome; pyroptosis; anti-inflammation; Chloranthus

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MDPI and ACS Style

Wen, Q.; Zhan, B.; Jin, L.; Peng, Z.; Liu, J.; Zhu, L.; Yang, D.; Xu, X.; Zhang, L.; Li, G.; et al. Chlojaponilactone B Attenuates THP-1 Macrophage Pyroptosis by Inhibiting the TLR/MyD88/NF-κB Pathway. Pharmaceuticals 2024, 17, 402. https://doi.org/10.3390/ph17030402

AMA Style

Wen Q, Zhan B, Jin L, Peng Z, Liu J, Zhu L, Yang D, Xu X, Zhang L, Li G, et al. Chlojaponilactone B Attenuates THP-1 Macrophage Pyroptosis by Inhibiting the TLR/MyD88/NF-κB Pathway. Pharmaceuticals. 2024; 17(3):402. https://doi.org/10.3390/ph17030402

Chicago/Turabian Style

Wen, Qiyin, Bingjinfeng Zhan, Lu Jin, Zijing Peng, Ju Liu, Longping Zhu, Depo Yang, Xinjun Xu, Lixia Zhang, Ge Li, and et al. 2024. "Chlojaponilactone B Attenuates THP-1 Macrophage Pyroptosis by Inhibiting the TLR/MyD88/NF-κB Pathway" Pharmaceuticals 17, no. 3: 402. https://doi.org/10.3390/ph17030402

APA Style

Wen, Q., Zhan, B., Jin, L., Peng, Z., Liu, J., Zhu, L., Yang, D., Xu, X., Zhang, L., Li, G., & Zhao, Z. (2024). Chlojaponilactone B Attenuates THP-1 Macrophage Pyroptosis by Inhibiting the TLR/MyD88/NF-κB Pathway. Pharmaceuticals, 17(3), 402. https://doi.org/10.3390/ph17030402

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