A New Pyrroloquinoline-Derivative-Based Fluorescent Probe for the Selective Detection and Cell Imaging of Lysine
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis of the Probe PQP-1
2.2. Fluorescent Response of Probe PQP-1 to Lysine
2.3. Selective Detection for Lysine
2.4. Proposed Response Mechanism
2.5. Imaging Study
2.6. Detection of Lysine Concentrations in Natural Mineral Water for Drinking
3. Materials and Methods
3.1. Materials and Apparatus
3.2. Preparation of the Probe PQP-1
3.3. Testing Conditions
3.4. Calculation of the Fluorescence Quantum Yield
Au = 0.0207, Fu = 27.036, n = 1.3330;
Quantum yield: Φu = 0.05.
3.5. Calculation of the Detection Limit
3.6. Imaging Study
3.7. Water Sample Preparation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Entry | Added Concentrations (μM) | Detected Concentrations (μM) | Recovery (%) |
---|---|---|---|
1 | 80 | 80.37 ± 1.43 | 100.46 |
2 | 220 | 224.25 ± 2.86 | 101.93 |
3 | 490 | 484.95 ± 0.45 | 98.97 |
4 | 750 | 724.84 ± 3.04 | 96.65 |
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Yang, B.; Zhou, J.; Huang, X.; Chen, Z.; Tian, S.; Shi, Y. A New Pyrroloquinoline-Derivative-Based Fluorescent Probe for the Selective Detection and Cell Imaging of Lysine. Pharmaceuticals 2022, 15, 474. https://doi.org/10.3390/ph15040474
Yang B, Zhou J, Huang X, Chen Z, Tian S, Shi Y. A New Pyrroloquinoline-Derivative-Based Fluorescent Probe for the Selective Detection and Cell Imaging of Lysine. Pharmaceuticals. 2022; 15(4):474. https://doi.org/10.3390/ph15040474
Chicago/Turabian StyleYang, Bing, Jiahua Zhou, Xu Huang, Zhongping Chen, Shu Tian, and Yujun Shi. 2022. "A New Pyrroloquinoline-Derivative-Based Fluorescent Probe for the Selective Detection and Cell Imaging of Lysine" Pharmaceuticals 15, no. 4: 474. https://doi.org/10.3390/ph15040474