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Article

T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures

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Área de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay
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Department of Pharmaceutical Sciences, Faculty of Chemistry, Universidad de la República, Montevideo 11800, Uruguay
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Área Física, DETEMA, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay
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Inmunología Clínica, (BIOCLIN) y Biotecnología, Polo Tecnológico de Pando-Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay
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Laboratorio de Experimentación Animal, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay
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Instituto IQUIMEFA UBA-CONICET, Departamento de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Buenos Aires C1113AAD, Argentina
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Instituto NANOBIOTEC UBA-CONICET, Departamento de Tecnología Farmacéutica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Buenos Aires C1113AAD, Argentina
*
Authors to whom correspondence should be addressed.
Academic Editors: Cristina Romero-López and Alfredo Berzal-Herranz
Pharmaceuticals 2022, 15(1), 15; https://doi.org/10.3390/ph15010015
Received: 8 November 2021 / Revised: 17 December 2021 / Accepted: 20 December 2021 / Published: 23 December 2021
(This article belongs to the Special Issue Potential of the Aptamers to Fill Therapeutic and Diagnostic Gaps)
Aptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve the delivery of this probe as well as create a novel drug delivery nanosystem targeted to the PTK7 receptor, we evaluate the co-association between the probe and preformed nanostructures. In this work, preformed pegylated liposomes (PPL) and linear and branched pristine polymeric micelles (PMs), based on PEO–PPO–PEO triblock copolymers were used: poloxamer F127® and poloxamines T1307® and T908®. For it, Sgc8-c-Alexa647 and its co-association with the different nanostructures was exhaustively analyzed. DLS analysis showed nanometric sizes, and TEM and AFM showed notable differences between free- and co-associated probe. Likewise, all nanosystems were evaluated on A20 lymphoma cell line overexpressing PTK7, and the confocal microscopy images showed distinctness in cellular uptake. Finally, the biodistribution in BALB/c mice bearing lymphoma-tumor and pharmacokinetic study revealed an encouraging profile for T908-probe. All data obtained from this work suggested that PMs and, more specifically T908 ones, are good candidates to improve the pharmacokinetics and the tumor uptake of aptamer-based probes. View Full-Text
Keywords: Sgc8-c aptamer; probe; polymeric micelles; liposomes; active targeting Sgc8-c aptamer; probe; polymeric micelles; liposomes; active targeting
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MDPI and ACS Style

Castelli, R.; Ibarra, M.; Faccio, R.; Miraballes, I.; Fernández, M.; Moglioni, A.; Cabral, P.; Cerecetto, H.; Glisoni, R.J.; Calzada, V. T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures. Pharmaceuticals 2022, 15, 15. https://doi.org/10.3390/ph15010015

AMA Style

Castelli R, Ibarra M, Faccio R, Miraballes I, Fernández M, Moglioni A, Cabral P, Cerecetto H, Glisoni RJ, Calzada V. T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures. Pharmaceuticals. 2022; 15(1):15. https://doi.org/10.3390/ph15010015

Chicago/Turabian Style

Castelli, Romina, Manuel Ibarra, Ricardo Faccio, Iris Miraballes, Marcelo Fernández, Albertina Moglioni, Pablo Cabral, Hugo Cerecetto, Romina J. Glisoni, and Victoria Calzada. 2022. "T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures" Pharmaceuticals 15, no. 1: 15. https://doi.org/10.3390/ph15010015

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