Next Article in Journal
COVID-19: A Brief Overview of the Discovery Clinical Trial
Previous Article in Journal
Study of the Potential of the Capsule Shell Based on Natural Polysaccharides in Targeted Delivery of the L-Phenylalanine Ammonia-Lyase Enzyme Preparation
Open AccessArticle

Oleacein and Foam Cell Formation in Human Monocyte-Derived Macrophages: A Potential Strategy against Early and Advanced Atherosclerotic Lesions

1
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
2
Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building 120 University Place, Glasgow G12 8TA, UK
3
Department of Internal and Agricultural Medicine, Jagiellonian University Medical College, 31-007 Krakow, Poland
4
Institute of Cardiovascular and Medical Sciences, University of Glasgow, BHF Centre for Excellence, 120 University Place, Glasgow G12 8TA, UK
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2020, 13(4), 64; https://doi.org/10.3390/ph13040064
Received: 20 March 2020 / Revised: 3 April 2020 / Accepted: 4 April 2020 / Published: 9 April 2020
(This article belongs to the Section Natural Products)
Background: Oleacein is a secoiridoid group polyphenol found mostly in Olea europea L. and Ligustrum vulgare L. (Oleaceae). The aim of the present study was to investigate a potential role of oleacein in prevention of the foam cell formation. Materials and Methods: Oleacein was isolated from Ligustrum vulgare leaves. Human monocyte-derived macrophages were obtained from monocytes cultured with Granulocyte-macrophage colony-stimulating factor (GM-CSF). Then, cells were incubated with 20 μM or 50 μM of oleacein and with oxidized low-density lipoprotein (oxLDL) (50 μg/mL). Visualization of lipid deposition within macrophages was carried out using Oil-Red-O. Expression of CD36, Scavenger receptor A1 (SRA1) and Lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) was determined by Reverse transcription polymerase chain reaction (RT-PCR) and by flow cytometry. Apoptosis was determined by flow cytometry using Annexin V assay. STAT3 and Acyl-coenzyme A: cholesterol acyltransferase type 1 (ACAT1) levels were determined by ELISA. P-STAT3, P-JAK1, P-JAK2 expressions were determined by Western blot (WB). Results: Oleacein in dose-dependent manner significantly reduced lipid deposits in macrophages as well as their expression of selected scavenger receptors. The highest decrease of expression was found for CD36 and SRA1 receptors, from above 20% to more than 75% compared to oxLDL and the lowest for LOX-1 receptor, from approx. 8% to approx. 25% compared to oxLDL-stimulated macrophages. Oleacein significantly reduced (2.5-fold) early apoptosis of oxLDL-stimulated macrophages. Moreover, oleacein significantly increased the protein expression of JAK/STAT3 pathway and had no effect on ACAT1 level. Conclusions: Our study demonstrates, for the first time, that oleacein inhibits foam cell formation in human monocyte-derived macrophages and thus can be a valuable tool in the prevention of early and advanced atherosclerotic lesions. View Full-Text
Keywords: oleacein; human monocyte-derived macrophages (hMDMs); foam cell formation; scavenger receptors (SR); JAK/STAT3 pathway; atherosclerosis oleacein; human monocyte-derived macrophages (hMDMs); foam cell formation; scavenger receptors (SR); JAK/STAT3 pathway; atherosclerosis
Show Figures

Graphical abstract

MDPI and ACS Style

Filipek, A.; Mikołajczyk, T.P.; Guzik, T.J.; Naruszewicz, M. Oleacein and Foam Cell Formation in Human Monocyte-Derived Macrophages: A Potential Strategy against Early and Advanced Atherosclerotic Lesions. Pharmaceuticals 2020, 13, 64.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop