Remyelination-Promoting DNA Aptamer Conjugate Myaptavin-3064 Binds to Adult Oligodendrocytes In Vitro
Abstract
:1. Introduction
2. Results
2.1. Characterization of HOG Cells in Culture
2.2. Specific Binding of Myaptavin-3064 to HOG Cells
2.3. Myaptavin-3064 Cellular Targets
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Antibodies
4.3. Preparation of Aptamer Complex
4.4. Animals
4.5. Cell Culture
4.6. Isolation of Mouse Mixed Cortical Cells
4.7. Adult Rat Mixed Glial Culture
4.8. Flow Cytometry
4.9. Immunocytochemistry
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Fereidan-Esfahani, M.; Yue, W.Y.; Wilbanks, B.; Johnson, A.J.; Warrington, A.E.; Howe, C.L.; Rodriguez, M.; Maher, L.J., III. Remyelination-Promoting DNA Aptamer Conjugate Myaptavin-3064 Binds to Adult Oligodendrocytes In Vitro. Pharmaceuticals 2020, 13, 403. https://doi.org/10.3390/ph13110403
Fereidan-Esfahani M, Yue WY, Wilbanks B, Johnson AJ, Warrington AE, Howe CL, Rodriguez M, Maher LJ III. Remyelination-Promoting DNA Aptamer Conjugate Myaptavin-3064 Binds to Adult Oligodendrocytes In Vitro. Pharmaceuticals. 2020; 13(11):403. https://doi.org/10.3390/ph13110403
Chicago/Turabian StyleFereidan-Esfahani, Mahboubeh, Wei Ying Yue, Brandon Wilbanks, Aaron J. Johnson, Arthur E. Warrington, Charles L. Howe, Moses Rodriguez, and Louis J. Maher, III. 2020. "Remyelination-Promoting DNA Aptamer Conjugate Myaptavin-3064 Binds to Adult Oligodendrocytes In Vitro" Pharmaceuticals 13, no. 11: 403. https://doi.org/10.3390/ph13110403