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Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

by Lide Gu 1, Wanli Yan 1, Le Liu 1, Shujun Wang 2,3, Xu Zhang 3,4 and Mingsheng Lyu 1,2,3,*
College of Marine Life and Fisheries, Huahai Institute of Technology, Lianyungang 222005, China
Marine Resources Development Institute of Jiangsu, Lianyungang 222005, China
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China
Verschuren Centre for Sustainability in Energy & the Environment, Cape Breton University, Sydney, NS B1P 6L2, Canada
Author to whom correspondence should be addressed.
Pharmaceuticals 2018, 11(2), 35;
Received: 8 March 2018 / Revised: 10 April 2018 / Accepted: 10 April 2018 / Published: 21 April 2018
(This article belongs to the Special Issue Aptamer-Based Diagnostics and Therapeutics)
Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer development could take more than 10 years, from one mutant cell to a visible tumor. Early diagnosis facilitates more effective treatment and leads to higher survival rate for cancer patients. Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic process that utilizes nuclease to generate long single stranded DNA (ssDNA) or RNA. The functional nucleic acid unit (aptamer, DNAzyme) could be replicated hundreds of times in a short period, and a lower LOD could be achieved if those units are combined with an enzymatic reaction, Surface Plasmon Resonance, electrochemical, or fluorescence detection, and other different kinds of biosensor. Multifarious RCA-based platforms have been developed to detect a variety of targets including DNA, RNA, SNP, proteins, pathogens, cytokines, micromolecules, and diseased cells. In this review, improvements in using the RCA technique for medical biosensors and biomedical applications were summarized and future trends in related research fields described. View Full-Text
Keywords: rolling circle amplification (RCA); biosensor; clinical diagnostics; cancer rolling circle amplification (RCA); biosensor; clinical diagnostics; cancer
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Gu, L.; Yan, W.; Liu, L.; Wang, S.; Zhang, X.; Lyu, M. Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing. Pharmaceuticals 2018, 11, 35.

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