An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
Abstract
:1. Introduction
2. Result and Discussion
2.1. The working potential
2.2. The effect of pH
2.3. The effect of Temperature
2.4. Substrate Concentration and Calibration Curves
2.5. The Reproducibility of the Enzyme Electrode
2.6. Storage Stability
2.7. Interference Effect
3. Experimental Section
3.1. Equipment and Reagents
3.2. Preparation of Uric Acid Biosensor
3.3. Amperometric Measurements
4. Conclusions
- Is usable in a large concentration range (2.5×0.10-6-8.5×0.10-5 M).
- Has a very low detection limit 1.0×0.10-6 M.
- Has an acceptable response time for a biosensor (70 second).
- Gives highly reproducible results (the electrode lost 4% of the initial amperometric response at the end of the 15th measurement).
- Has a satisfactory storage stabilization (the electrode lost 20% of the initial amperometric response at the end of the 4th week).
- The Km (app) value of uricase enzyme immobilized in polyaniline-polypyrrole film was 1.57 mM.
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Interfering substances | Concentration | Interference effect of Substances |
---|---|---|
Ascorbic acid | 1.0×10- M | - |
Glucose | 5.0×10- M | - |
Bilirubin | 1.0×10- M | - |
Urea | 1.0×10- M | - |
Paracetamol | 1.0×10- M | 31% |
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Arslan, F. An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film. Sensors 2008, 8, 5492-5500. https://doi.org/10.3390/s8095492
Arslan F. An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film. Sensors. 2008; 8(9):5492-5500. https://doi.org/10.3390/s8095492
Chicago/Turabian StyleArslan, Fatma. 2008. "An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film" Sensors 8, no. 9: 5492-5500. https://doi.org/10.3390/s8095492
APA StyleArslan, F. (2008). An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film. Sensors, 8(9), 5492-5500. https://doi.org/10.3390/s8095492