Next Article in Journal
Remote Sensing of Agro-droughts in Guangdong Province of China Using MODIS Satellite Data
Previous Article in Journal
Performance and Transient Behavior of Vertically Integrated Thin-film Silicon Sensors
Article Menu

Export Article

Open AccessReview
Sensors 2008, 8(8), 4669-4686;

Antibody Fragments as Probe in Biosensor Development

Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium
Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
Department of Molecular Biology, Technologiepark 927, B-9052 Zwijnaarde, Ghent University, Ghent, Belgium
Department for Molecular Biomedical Research, VIB, Ghent, Belgium
IMEC, NEXT, Kapeldreef 75, B-3001 Leuven, Belgium
Author to whom correspondence should be addressed.
Received: 7 July 2008 / Revised: 6 August 2008 / Accepted: 7 August 2008 / Published: 8 August 2008
Full-Text   |   PDF [329 KB, uploaded 21 June 2014]


Today’s proteomic analyses are generating increasing numbers of biomarkers, making it essential to possess highly specific probes able to recognize those targets. Antibodies are considered to be the first choice as molecular recognition units due to their target specificity and affinity, which make them excellent probes in biosensor development. However several problems such as difficult directional immobilization, unstable behavior, loss of specificity and steric hindrance, may arise from using these large molecules. Luckily, protein engineering techniques offer designed antibody formats suitable for biomarker analysis. Minimization strategies of antibodies into Fab fragments, scFv or even single-domain antibody fragments like VH, VL or VHHs are reviewed. Not only the size of the probe but also other issues like choice of immobilization tag, type of solid support and probe stability are of critical importance in assay development for biosensing. In this respect, multiple approaches to specifically orient and couple antibody fragments in a generic one-step procedure directly on a biosensor substrate are discussed. View Full-Text
Keywords: display technology; affinity; stability; immobilization; immunoassay. display technology; affinity; stability; immobilization; immunoassay.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Saerens, D.; Huang, L.; Bonroy, K.; Muyldermans, S. Antibody Fragments as Probe in Biosensor Development. Sensors 2008, 8, 4669-4686.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top