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Sensors 2006, 6(11), 1513-1522;

Modelling of Amperometric Biosensors in the Case of Substrate Inhibition

Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio al. 11, 10223 Vilnius, Lithuania
Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT−03225 Vilnius, Lithuania
Author to whom correspondence should be addressed.
Received: 15 September 2006 / Accepted: 6 November 2006 / Published: 7 November 2006
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The response of an amperometric biosensor at mixed enzyme kinetics anddiffusion limitations was modelled digitally in the case of substrate inhibition. Digitalsimulations were carried out using a finite difference technique. Calculations showedcomplex kinetics of biosensor response. At low enzyme activity and substrate concentration(S0), the response of the sensor looks like it is limited by a simple substrate diffusion. Atsubstrate concentration comparable to the Michaelis-Menten constant (KM), the responsechange shows a maximal value. A sharp response change was indicated at high enzymeactivity and high (4.9 > S0/KM > 4.5) substrate concentration. This was explained by multiconcentrationof substrate generation inside the enzyme layer. This conclusion wasconfirmed by the analytical solution of the simplified biosensor model with externaldiffusion limitation at steady-state conditions. The complex kinetics of response changeproduces different calibration graphs for biosensor response at transition and steady state. View Full-Text
Keywords: biosensor; enzyme inhibition; diffusion; modelling biosensor; enzyme inhibition; diffusion; modelling
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kulys, J.; Baronas, R. Modelling of Amperometric Biosensors in the Case of Substrate Inhibition. Sensors 2006, 6, 1513-1522.

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