Can EMG-Derived Upper Limb Muscle Synergies Serve as Markers for Post-Stroke Motor Assessment and Prediction of Rehabilitation Outcome?

EMG-derived muscle synergy, as a representation of neuromotor modules utilized for motor control, has been proposed as a biomarker for stroke rehabilitation. Here, we evaluate the utility of muscle synergies for assessing motor function and predicting post-intervention motor outcome in a stroke rehabilitation clinical trial. Subacute stroke survivors (n = 59) received monthlong acupuncture (Acu), sham acupuncture (ShamAcu) or no acupuncture (NoAcu) as adjunctive rehabilitative intervention alongside standard physiotherapy. Clinical scores and EMGs (14 muscles, eight motor tasks) were collected from the stroke-affected upper limb before and after intervention. We then extracted muscle synergies from EMGs using non-negative matrix factorization and designed 12 muscle synergy indices (MSIs) to summarize different aspects of post-stroke synergy features. All MSIs correlated with multiple clinical scores, suggesting that our indices could potentially serve as biomarkers for post-stroke motor functional assessments. While the intervention groups did not differ in their pre-to-post differences in the clinical scores, the inclusion of MSIs into analysis revealed that on average Acu promoted more recovery of synergy features than ShamAcu and NoAcu, though not all subjects in the group were Acu responders. We then built regression models using pre-intervention MSIs and clinical variables to predict the outcomes of Acu and NoAcu and showed by a preliminary retrospective simulation of patient stratification that MSI-based predictions could have led to better post-intervention motor improvement. Overall, we demonstrate that muscle synergies can potentially clarify the effects of interventions and assist in motor assessment, outcome prediction, and treatment selection.