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An Ultrahigh Sensitive Microwave Microfluidic System for Fast and Continuous Measurements of Liquid Solution Concentrations
Article

Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach

1
Department of Electrical and Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, Canada
2
Dartmouth Ocean Technologies Inc., 25 Parker Street, Dartmouth, NS B2Y 4T5, Canada
3
Department of Process Engineering and Applied Science, Dalhousie University, 5410 Spring Garden Road, Halifax, NS B3J 1B6, Canada
*
Author to whom correspondence should be addressed.
The authors contributed equally.
Academic Editors: Navid Kashaninejad and Md Nazmul Islam
Sensors 2021, 21(18), 6250; https://doi.org/10.3390/s21186250
Received: 23 August 2021 / Revised: 14 September 2021 / Accepted: 15 September 2021 / Published: 17 September 2021
(This article belongs to the Special Issue Microfluidic Sensors 2020-2021)
A novel microfluidic optical cell is presented that enables simultaneous measurement of both light absorbance and fluorescence on microlitre volumes of fluid. The chip design is based on an inlaid fabrication technique using clear and opaque poly(methyl methacrylate) or PMMA to create a 20.2 mm long optical cell. The inlaid approach allows fluid interrogation with minimal interference from external light over centimeter long path lengths. The performance of the optical cell is evaluated using a stable fluorescent dye: rhodamine B. Excellent linear relationships (R2 > 0.99) are found for both absorbance and fluorescence over a 0.1–10 µM concentration range. Furthermore, the molar attenuation spectrum is accurately measured over the range 460–550 nm. The approach presented here is applicable to numerous colorimetric- or fluorescence-based assays and presents an important step in the development of multipurpose lab-on-chip sensors. View Full-Text
Keywords: lab-on-chip; microfluidic; PMMA; rhodamine; light absorbance; fluorescence; sensor lab-on-chip; microfluidic; PMMA; rhodamine; light absorbance; fluorescence; sensor
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MDPI and ACS Style

Creelman, J.J.; Luy, E.A.; Beland, G.C.H.; Sonnichsen, C.; Sieben, V.J. Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach. Sensors 2021, 21, 6250. https://doi.org/10.3390/s21186250

AMA Style

Creelman JJ, Luy EA, Beland GCH, Sonnichsen C, Sieben VJ. Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach. Sensors. 2021; 21(18):6250. https://doi.org/10.3390/s21186250

Chicago/Turabian Style

Creelman, Joshua J., Edward A. Luy, Gabryelle C.H. Beland, Colin Sonnichsen, and Vincent J. Sieben 2021. "Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach" Sensors 21, no. 18: 6250. https://doi.org/10.3390/s21186250

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