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Article

Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes

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Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy
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NanoBioAnalysis Group, Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain
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BioNanoAnalytical Spectrometry and Electrochemistry Group, Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(8), 2349; https://doi.org/10.3390/s20082349
Received: 31 March 2020 / Revised: 16 April 2020 / Accepted: 18 April 2020 / Published: 20 April 2020
(This article belongs to the Section Biosensors)
Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because of this, the development of point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become of outstanding importance. According to this, electrochemical aptasensors have been demonstrated as useful tools to quantify a broad variety of targets. In this work, we develop a simple methodology for the determination of β-lactoglobulin (β-LG) in food samples using a folding-based electrochemical aptasensor built on poly-L-lysine modified graphite screen-printed electrodes (GSPEs) and an anti-β-lactoglobulin aptamer tagged with methylene blue (MB). This aptamer changes its conformation when the sample contains β-LG, and due to this, the spacing between MB and the electrode surface (and therefore the electron transfer efficiency) also changes. The response of this biosensor was linear for concentrations of β-LG within the range 0.1–10 ng·mL−1, with a limit of detection of 0.09 ng·mL−1. The biosensor was satisfactorily employed for the determination of spiked β-LG in real food samples. View Full-Text
Keywords: β-lactoglobulin; folding-based aptasensor; poly-L-lysine; conducting polymers; methylene blue β-lactoglobulin; folding-based aptasensor; poly-L-lysine; conducting polymers; methylene blue
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MDPI and ACS Style

Amor-Gutiérrez, O.; Selvolini, G.; Fernández-Abedul, M.T.; de la Escosura-Muñiz, A.; Marrazza, G. Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes. Sensors 2020, 20, 2349. https://doi.org/10.3390/s20082349

AMA Style

Amor-Gutiérrez O, Selvolini G, Fernández-Abedul MT, de la Escosura-Muñiz A, Marrazza G. Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes. Sensors. 2020; 20(8):2349. https://doi.org/10.3390/s20082349

Chicago/Turabian Style

Amor-Gutiérrez, Olaya, Giulia Selvolini, M. T. Fernández-Abedul, Alfredo de la Escosura-Muñiz, and Giovanna Marrazza. 2020. "Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes" Sensors 20, no. 8: 2349. https://doi.org/10.3390/s20082349

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