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Open AccessArticle

Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles

1
Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nankang, Taipei 115, Taiwan
2
Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo 113-8549, Japan
3
Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan
4
Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(23), 6827; https://doi.org/10.3390/s20236827
Received: 1 October 2020 / Revised: 26 November 2020 / Accepted: 27 November 2020 / Published: 29 November 2020
(This article belongs to the Special Issue Machine Olfaction and Taste)
Our groups have previously developed a biochemical gas sensor to measure isopropanol (IPA) in exhaled air and have applied it for breath IPA investigation in healthy subjects and diabetes patients. In this study, the original bio-sniffer was modified with a series of components that improved the limit of detection (LOD). First, the modified IPA bio-sniffer used a C8855-type photomultiplier tube (PMT) that performed well in the photon sensitivity at the peak wavelength of nicotinamide adenine dinucleotide (NADH) fluorescence. Second, the multi-core bifurcated optical fiber, which incorporated 36 fibers to replace the previous dual-core type, enhanced the fluorescence collection. Third, the optical fiber probe was reinforced for greater width, and the flow-cell was redesigned to increase the area of the enzyme-immobilized membrane in contact with the air sample. These modifications lowered the detection limit to 0.5 ppb, a significant increase over the previous 1.0 ppb. Moreover, the modified bio-sniffer successfully analyzed the IPA concentration in exhaled air from a volunteer, which confirmed its capability for real-world sample detection. The modified bio-sniffer is more applicable to breath measurement and the detection of other extremely-low-concentration samples. View Full-Text
Keywords: breath isopropanol; biosensor; NADH; secondary alcohol dehydrogenase; volatile organic compounds; gas sensor breath isopropanol; biosensor; NADH; secondary alcohol dehydrogenase; volatile organic compounds; gas sensor
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MDPI and ACS Style

Chien, P.-J.; Suzuki, T.; Ye, M.; Toma, K.; Arakawa, T.; Iwasaki, Y.; Mitsubayashi, K. Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles. Sensors 2020, 20, 6827. https://doi.org/10.3390/s20236827

AMA Style

Chien P-J, Suzuki T, Ye M, Toma K, Arakawa T, Iwasaki Y, Mitsubayashi K. Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles. Sensors. 2020; 20(23):6827. https://doi.org/10.3390/s20236827

Chicago/Turabian Style

Chien, Po-Jen; Suzuki, Takuma; Ye, Ming; Toma, Koji; Arakawa, Takahiro; Iwasaki, Yasuhiko; Mitsubayashi, Kohji. 2020. "Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles" Sensors 20, no. 23: 6827. https://doi.org/10.3390/s20236827

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