Next Article in Journal
Triaxial Accelerometer-Based Falls and Activities of Daily Life Detection Using Machine Learning
Next Article in Special Issue
Rapid Detection of β-Lactamase-Producing Bacteria Using the Integrated Comprehensive Droplet Digital Detection (IC 3D) System
Previous Article in Journal
Multitask Learning and GCN-Based Taxi Demand Prediction for a Traffic Road Network
Previous Article in Special Issue
Continuous-Flow Separation of Magnetic Particles from Biofluids: How Does the Microdevice Geometry Determine the Separation Performance?
Article

An Interface–Particle Interaction Approach for Evaluation of the Co-Encapsulation Efficiency of Cells in a Flow-Focusing Droplet Generator

1
Department of Mechanical Engineering, Sharif University of Technology, Azadi St., Tehran 11155, Iran
2
Department of Mechanical Engineering, University of Waterloo, 200 University Avenue West, N2L 3G, Waterloo, ON N2L 3G1, Canada
3
Queensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane QLD 4111, Australia
*
Authors to whom correspondence should be addressed.
Sensors 2020, 20(13), 3774; https://doi.org/10.3390/s20133774
Received: 13 May 2020 / Revised: 28 June 2020 / Accepted: 1 July 2020 / Published: 5 July 2020
(This article belongs to the Special Issue Lab-on-a-Chip and Microfluidic Sensors)
Droplet-based microfluidics offers significant advantages, such as high throughput and scalability, making platforms based on this technology ideal candidates for point-of-care (POC) testing and clinical diagnosis. However, the efficiency of co-encapsulation in droplets is suboptimal, limiting the applicability of such platforms for the biosensing applications. The homogeneity of the bioanalytes in the droplets is an unsolved problem. While there is extensive literature on the experimental setups and active methods used to increase the efficiency of such platforms, passive techniques have received less attention, and their fundamentals have not been fully explored. Here, we develop a novel passive technique for investigating cell encapsulation using the finite element method (FEM). The level set method was used to track the interfaces of forming droplets. The effects of walls and the droplet interfaces on relatively large cells were calculated to track them more accurately during encapsulation. The static surface tension force was used to account for the effects of the interfaces on cells. The results revealed that the pairing efficiency is highly sensitive to the standard deviation (SD) of the distance between the cells in the entrance channel. The pairing efficiency prediction error of our model differed by less than 5% from previous experiments. The proposed model can be used to evaluate the performance of droplet-based microfluidic devices to ensure higher precision for co-encapsulation of cells. View Full-Text
Keywords: finite element method; droplet generator; microfluidics; encapsulation efficiency; flow focusing; particle interaction finite element method; droplet generator; microfluidics; encapsulation efficiency; flow focusing; particle interaction
Show Figures

Figure 1

MDPI and ACS Style

Yaghoobi, M.; Saidi, M.S.; Ghadami, S.; Kashaninejad, N. An Interface–Particle Interaction Approach for Evaluation of the Co-Encapsulation Efficiency of Cells in a Flow-Focusing Droplet Generator. Sensors 2020, 20, 3774. https://doi.org/10.3390/s20133774

AMA Style

Yaghoobi M, Saidi MS, Ghadami S, Kashaninejad N. An Interface–Particle Interaction Approach for Evaluation of the Co-Encapsulation Efficiency of Cells in a Flow-Focusing Droplet Generator. Sensors. 2020; 20(13):3774. https://doi.org/10.3390/s20133774

Chicago/Turabian Style

Yaghoobi, Mohammad; Saidi, Mohammad S.; Ghadami, Sepehr; Kashaninejad, Navid. 2020. "An Interface–Particle Interaction Approach for Evaluation of the Co-Encapsulation Efficiency of Cells in a Flow-Focusing Droplet Generator" Sensors 20, no. 13: 3774. https://doi.org/10.3390/s20133774

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop