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Article

Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements

1
Faculty of Biomedical Engineering, Czech Technical University in Prague, 27201 Kladno, Czech Republic
2
Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA
3
Department of Cardiology, Regional Hospital Liberec, 46063 Liberec, Czech Republic
4
Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, 40096 Ústí nad Labem, Czech Republic
5
Institute of Chemical Process Fundamentals of the ASCR, 16502 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(11), 3237; https://doi.org/10.3390/s20113237
Received: 11 May 2020 / Revised: 31 May 2020 / Accepted: 4 June 2020 / Published: 6 June 2020
(This article belongs to the Special Issue Fiber Optic Sensors in Chemical and Biological Applications)
In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used. View Full-Text
Keywords: whole-cell biosensor; bioluminescent bioreporter; optical fiber biosensor; toluene; Pseudomonas putida TVA8; Escherichia coli 652T7 whole-cell biosensor; bioluminescent bioreporter; optical fiber biosensor; toluene; Pseudomonas putida TVA8; Escherichia coli 652T7
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MDPI and ACS Style

Zajíc, J.; Ripp, S.; Trögl, J.; Kuncová, G.; Pospíšilová, M. Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements. Sensors 2020, 20, 3237. https://doi.org/10.3390/s20113237

AMA Style

Zajíc J, Ripp S, Trögl J, Kuncová G, Pospíšilová M. Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements. Sensors. 2020; 20(11):3237. https://doi.org/10.3390/s20113237

Chicago/Turabian Style

Zajíc, Jakub, Steven Ripp, Josef Trögl, Gabriela Kuncová, and Marie Pospíšilová. 2020. "Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements" Sensors 20, no. 11: 3237. https://doi.org/10.3390/s20113237

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