Next Article in Journal
Near-Infrared Transmittance Spectral Imaging for Nondestructive Measurement of Internal Disorder in Korean Ginseng
Previous Article in Journal
A Framework for Multiple Ground Target Finding and Inspection Using a Multirotor UAS
Open AccessArticle

Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water

1
Department of Physics “Ettore Pancini”, University of Naples “Federico II”, Via Cinthia, 26, 80126 Naples, Italy
2
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055 Portici Naples, Italy
3
Department of Agriculture, University of Naples “Federico II”, Via Università, 133, 80055 Portici Naples, Italy
4
Department of Chemical Sciences, University of Naples “Federico II”, Via Cinthia, 26, 80126 Naples, Italy
5
Cosvitech Società Consortile a Responsabilità Limitata, 80142 Naples, Italy
6
Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci, 32, 20133 Milano, Italy
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(1), 274; https://doi.org/10.3390/s20010274
Received: 29 November 2019 / Revised: 27 December 2019 / Accepted: 30 December 2019 / Published: 3 January 2020
(This article belongs to the Section Biosensors)
The development of a simple and low cost electrochemical impedance immunosensor based on screen printed gold electrode for rapid detection of Escherichia coli in water is reported. The immunosensor is fabricated by immobilizing anti-E. coli antibodies onto a gold surface in a covalent way by the photochemical immobilization technique, a simple procedure able to bind antibodies upright onto gold surfaces. Impedance spectra are recorded in 0.01 M phosphate buffer solution (PBS) containing 10 mM Fe(CN)63−/Fe(CN)64− as redox probe. The Nyquist plots can be modelled with a modified Randles circuit, identifying the charge transfer resistance Rct as the relevant parameter after the immobilization of antibodies, the blocking with BSA and the binding of E. coli. The introduction of a standard amplification procedure leads to a significant enhancement of the impedance increase, which allows one to measure E. coli in drinking water with a limit of detection of 3 × 101 CFU mL−1 while preserving the rapidity of the method that requires only 1 h to provide a “yes/no” response. Additionally, by applying the Langmuir adsorption model, we are able to describe the change of Rct in terms of the “effective” electrode, which is modified by the detection of the analyte whose microscopic conducting properties can be quantified. View Full-Text
Keywords: Escherichia coli; immunosensor; electrochemical impedance spectroscopy; antibodies; photochemical immobilization technique; cyclic voltammetry Escherichia coli; immunosensor; electrochemical impedance spectroscopy; antibodies; photochemical immobilization technique; cyclic voltammetry
Show Figures

Figure 1

MDPI and ACS Style

Cimafonte, M.; Fulgione, A.; Gaglione, R.; Papaianni, M.; Capparelli, R.; Arciello, A.; Bolletti Censi, S.; Borriello, G.; Velotta, R.; Della Ventura, B. Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water. Sensors 2020, 20, 274. https://doi.org/10.3390/s20010274

AMA Style

Cimafonte M, Fulgione A, Gaglione R, Papaianni M, Capparelli R, Arciello A, Bolletti Censi S, Borriello G, Velotta R, Della Ventura B. Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water. Sensors. 2020; 20(1):274. https://doi.org/10.3390/s20010274

Chicago/Turabian Style

Cimafonte, Martina; Fulgione, Andrea; Gaglione, Rosa; Papaianni, Marina; Capparelli, Rosanna; Arciello, Angela; Bolletti Censi, Sergio; Borriello, Giorgia; Velotta, Raffaele; Della Ventura, Bartolomeo. 2020. "Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water" Sensors 20, no. 1: 274. https://doi.org/10.3390/s20010274

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop