Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water

Cimafonte, Martina; Fulgione, Andrea; Gaglione, Rosa; Papaianni, Marina; Capparelli, Rosanna; Arciello, Angela; Bolletti Censi, Sergio; Borriello, Giorgia; Velotta, Raffaele; Della Ventura, Bartolomeo

doi:10.3390/s20010274

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Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water

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⁴,

³,

⁴,

Sergio Bolletti Censi

⁵,

Giorgia Borriello

Raffaele Velotta

and

Bartolomeo Della Ventura

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Department of Physics “Ettore Pancini”, University of Naples “Federico II”, Via Cinthia, 26, 80126 Naples, Italy

Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055 Portici Naples, Italy

Department of Agriculture, University of Naples “Federico II”, Via Università, 133, 80055 Portici Naples, Italy

⁴

Department of Chemical Sciences, University of Naples “Federico II”, Via Cinthia, 26, 80126 Naples, Italy

⁵

Cosvitech Società Consortile a Responsabilità Limitata, 80142 Naples, Italy

⁶

Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci, 32, 20133 Milano, Italy

Author to whom correspondence should be addressed.

Sensors 2020, 20(1), 274; https://doi.org/10.3390/s20010274

Received: 29 November 2019 / Revised: 27 December 2019 / Accepted: 30 December 2019 / Published: 3 January 2020

(This article belongs to the Section Biosensors)

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Abstract

The development of a simple and low cost electrochemical impedance immunosensor based on screen printed gold electrode for rapid detection of Escherichia coli in water is reported. The immunosensor is fabricated by immobilizing anti-E. coli antibodies onto a gold surface in a covalent way by the photochemical immobilization technique, a simple procedure able to bind antibodies upright onto gold surfaces. Impedance spectra are recorded in 0.01 M phosphate buffer solution (PBS) containing 10 mM Fe(CN)₆³⁻/Fe(CN)₆⁴⁻ as redox probe. The Nyquist plots can be modelled with a modified Randles circuit, identifying the charge transfer resistance R_ct as the relevant parameter after the immobilization of antibodies, the blocking with BSA and the binding of E. coli. The introduction of a standard amplification procedure leads to a significant enhancement of the impedance increase, which allows one to measure E. coli in drinking water with a limit of detection of 3 × 10¹ CFU mL⁻¹ while preserving the rapidity of the method that requires only 1 h to provide a “yes/no” response. Additionally, by applying the Langmuir adsorption model, we are able to describe the change of R_ct in terms of the “effective” electrode, which is modified by the detection of the analyte whose microscopic conducting properties can be quantified. View Full-Text

Keywords: Escherichia coli; immunosensor; electrochemical impedance spectroscopy; antibodies; photochemical immobilization technique; cyclic voltammetry

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Cimafonte, M.; Fulgione, A.; Gaglione, R.; Papaianni, M.; Capparelli, R.; Arciello, A.; Bolletti Censi, S.; Borriello, G.; Velotta, R.; Della Ventura, B. Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water. Sensors 2020, 20, 274. https://doi.org/10.3390/s20010274

AMA Style

Cimafonte M, Fulgione A, Gaglione R, Papaianni M, Capparelli R, Arciello A, Bolletti Censi S, Borriello G, Velotta R, Della Ventura B. Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water. Sensors. 2020; 20(1):274. https://doi.org/10.3390/s20010274

Chicago/Turabian Style

Cimafonte, Martina, Andrea Fulgione, Rosa Gaglione, Marina Papaianni, Rosanna Capparelli, Angela Arciello, Sergio Bolletti Censi, Giorgia Borriello, Raffaele Velotta, and Bartolomeo Della Ventura. 2020. "Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water" Sensors 20, no. 1: 274. https://doi.org/10.3390/s20010274

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Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water

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