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Open AccessArticle

A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria

1
Institute of Nano- and Biotechnologies (INB), FH Aachen, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany
2
Department of Physics and Astronomy, Laboratory for Soft Matter and Biophysics, KU Leuven, Celestijnenlaan 200 D, 3001 Leuven, Belgium
3
Institute of Complex Systems (ICS-8), Research Centre Jülich GmbH, Wilhelm-Johnen-Straße 1, 52425 Jülich, Germany
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(21), 4692; https://doi.org/10.3390/s19214692
Received: 10 October 2019 / Revised: 23 October 2019 / Accepted: 24 October 2019 / Published: 29 October 2019
Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process. View Full-Text
Keywords: light-addressable potentiometric sensor (LAPS); Lactobacillus brevis; Escherichia coli; Corynebacterium glutamicum; cellular metabolism; differential cell-based measurement; multi-analyte analysis; extracellular acidification light-addressable potentiometric sensor (LAPS); Lactobacillus brevis; Escherichia coli; Corynebacterium glutamicum; cellular metabolism; differential cell-based measurement; multi-analyte analysis; extracellular acidification
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MDPI and ACS Style

Dantism, S.; Röhlen, D.; Wagner, T.; Wagner, P.; Schöning, M.J. A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria. Sensors 2019, 19, 4692. https://doi.org/10.3390/s19214692

AMA Style

Dantism S, Röhlen D, Wagner T, Wagner P, Schöning MJ. A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria. Sensors. 2019; 19(21):4692. https://doi.org/10.3390/s19214692

Chicago/Turabian Style

Dantism, Shahriar; Röhlen, Désirée; Wagner, Torsten; Wagner, Patrick; Schöning, Michael J. 2019. "A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria" Sensors 19, no. 21: 4692. https://doi.org/10.3390/s19214692

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