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Open AccessArticle

Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite

1
Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, France
2
Laboratory of Advanced Materials and Interfaces, University of Monastir, 5000 Monastir, Tunisia
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(1), 154; https://doi.org/10.3390/s19010154
Received: 2 December 2018 / Revised: 20 December 2018 / Accepted: 27 December 2018 / Published: 4 January 2019
(This article belongs to the Section Biosensors)
In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) (Chitosan/kappa-carrageenan) doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (µA) as a function of the log of the glucose concentration (log [glucose]), shows three times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 µM and a linearity range between 10 µM and 7 mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability, and negligible interfering effects from ascorbic acid, uric acid, urea, and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained. View Full-Text
Keywords: chitosan; kappa-carrageenan; polyelectrolyte complex; gold nanoparticles encapsulation; glucose oxidase; bionanocomposite chitosan; kappa-carrageenan; polyelectrolyte complex; gold nanoparticles encapsulation; glucose oxidase; bionanocomposite
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MDPI and ACS Style

Rassas, I.; Braiek, M.; Bonhomme, A.; Bessueille, F.; Raffin, G.; Majdoub, H.; Jaffrezic-Renault, N. Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite. Sensors 2019, 19, 154.

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