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Article

Three-Dimensional High-Resolution Digital Inline Hologram Reconstruction with a Volumetric Deconvolution Method

by 1 and 2,*
1
Micro Mechanical System Technology Laboratory, Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
2
Sensors and Aerosol Laboratory, Department of Mechanical, Aerospace and Nuclear Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Korea
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(9), 2918; https://doi.org/10.3390/s18092918
Received: 20 July 2018 / Revised: 29 August 2018 / Accepted: 30 August 2018 / Published: 3 September 2018
(This article belongs to the Special Issue Portable Biosensing Systems for Point-of-Care Diagnostic Applications)
The digital in-line holographic microscope (DIHM) was developed for a 2D imaging technology and has recently been adapted to 3D imaging methods, providing new approaches to obtaining volumetric images with both a high resolution and wide field-of-view (FOV), which allows the physical limitations to be overcome. However, during the sectioning process of 3D image generation, the out-of-focus image of the object becomes a significant impediment to obtaining evident 3D features in the 2D sectioning plane of a thick biological sample. Based on phase retrieved high-resolution holographic imaging and a 3D deconvolution technique, we demonstrate that a high-resolution 3D volumetric image, which significantly reduces wave-front reconstruction and out-of-focus artifacts, can be achieved. The results show a 3D volumetric image that is more finely focused compared to a conventional 3D stacked image from 2D reconstructed images in relation to micron-size polystyrene beads, a whole blood smear, and a kidney tissue sample. We believe that this technology can be applicable for medical-grade images of smeared whole blood or an optically cleared tissue sample for mobile phytological microscopy and laser sectioning microscopy. View Full-Text
Keywords: digital holography; holography microscope; volumetric deconvolution; three-dimensional volumetric deconvolution digital holography; holography microscope; volumetric deconvolution; three-dimensional volumetric deconvolution
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MDPI and ACS Style

Eom, J.; Moon, S. Three-Dimensional High-Resolution Digital Inline Hologram Reconstruction with a Volumetric Deconvolution Method. Sensors 2018, 18, 2918. https://doi.org/10.3390/s18092918

AMA Style

Eom J, Moon S. Three-Dimensional High-Resolution Digital Inline Hologram Reconstruction with a Volumetric Deconvolution Method. Sensors. 2018; 18(9):2918. https://doi.org/10.3390/s18092918

Chicago/Turabian Style

Eom, Junseong, and Sangjun Moon. 2018. "Three-Dimensional High-Resolution Digital Inline Hologram Reconstruction with a Volumetric Deconvolution Method" Sensors 18, no. 9: 2918. https://doi.org/10.3390/s18092918

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