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Article

A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAP

1
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab., Universidad Politécnica de Madrid, Campus Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain
2
Department of Applied Physics and Materials Engineering, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutierrez Abascal 2, 28006 Madrid, Spain
3
CQS Laboratory, Calle Marie Curie, 5, Rivas-Vaciamadrid, 28521 Madrid, Spain
4
BioOptical Detection SL, Centro de Empresas, Campus Montegancedo, 28223 Madrid, Spain
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(8), 2686; https://doi.org/10.3390/s18082686
Received: 22 June 2018 / Revised: 3 August 2018 / Accepted: 13 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Label-Free Biosensors)
Food allergy is a common disease worldwide with over 6% of the population (200–250 million people) suffering from any food allergy nowadays. The most dramatic increase seems to be happening in children and young people. Therefore, improvements in the diagnosis efficiency of these diseases are needed. Immunoglobulin type E (IgE) biomarker determination in human serum is a typical in vitro test for allergy identification. In this work, we used a novel biosensor based on label-free photonic transducers called BICELLs (Biophotonic Sensing Cells) for IgE detection. These BICELLs have a thin film of nitrocellulose over the sensing surface, they can be vertical optically interrogated, and are suitable for being integrated on a chip. The BICELLs sensing surface sizes used were 100 and 800 µm in diameter. We obtained calibration curves with IgE standards by immobilizating anti-IgE antibodies and identified with standard IgE calibrators in minute sample amounts (3 µL). The results, in similar assay format, were compared with commercially available ImmunoCAP®. The versatility of the interferometric nitrocellulose-based sensing surface was demonstrated since the limit of detections for BICELLs and ImmunoCAP® were 0.7 and 0.35 kU/L, respectively. View Full-Text
Keywords: label-free biosensor; nitrocellulose; IgE; calibration curves; ImmunoCAP®, allergy diagnostic label-free biosensor; nitrocellulose; IgE; calibration curves; ImmunoCAP®, allergy diagnostic
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MDPI and ACS Style

Espinosa, R.L.; Laguna, M.F.; Fernández, F.; Santamaria, B.; Sanza, F.J.; Maigler, M.V.; Álvarez-Millán, J.J.; Canalejas-Tejero, V.; Holgado, M. A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAP. Sensors 2018, 18, 2686. https://doi.org/10.3390/s18082686

AMA Style

Espinosa RL, Laguna MF, Fernández F, Santamaria B, Sanza FJ, Maigler MV, Álvarez-Millán JJ, Canalejas-Tejero V, Holgado M. A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAP. Sensors. 2018; 18(8):2686. https://doi.org/10.3390/s18082686

Chicago/Turabian Style

Espinosa, Rocio L., María Fe Laguna, Fátima Fernández, Beatriz Santamaria, Francisco Javier Sanza, Maria Victoria Maigler, Juan J. Álvarez-Millán, Víctor Canalejas-Tejero, and Miguel Holgado. 2018. "A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAP" Sensors 18, no. 8: 2686. https://doi.org/10.3390/s18082686

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