Next Article in Journal
Remote Sensing of Wildland Fire-Induced Risk Assessment at the Community Level
Next Article in Special Issue
Aptamer-Based Biosensors to Detect Aquatic Phycotoxins and Cyanotoxins
Previous Article in Journal
A Comparison of ACQ, AIE and AEE-Based Polymers Loaded on Polyurethane Foams as Sensors for Explosives Detection
Previous Article in Special Issue
Detection of Metallothionein in Javanese Medaka (Oryzias javanicus), Using a scFv-Immobilized Protein Chip
Open AccessArticle

A High Aspect Ratio Bifurcated 128-Microchannel Microfluidic Device for Environmental Monitoring of Explosives

Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, DC 20375, USA
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(5), 1568; https://doi.org/10.3390/s18051568
Received: 27 March 2018 / Revised: 9 May 2018 / Accepted: 10 May 2018 / Published: 15 May 2018
(This article belongs to the Special Issue Environmental Monitoring Biosensors)
Design and evolution of explosives monitoring and detection platforms to address the challenges of trace level chemical identification have led investigations into the use of intricately designed microfluidic devices. Microfluidic devices are unique tools that possess distinct characteristics that, when designed properly and configured with optical and fluidic components, can produce detection platforms with unmatched performance levels. Herein, we report the design, fabrication and integration of a bifurcated high aspect ratio microfluidic device containing 128 microchannels (40 mm × 40 μm × 250 μm; L × W × H) for explosives detection at trace levels. Aspect ratios measuring >6:1 support improved receptor-target molecule interactions, higher throughput and extremely low limits of detection (LOD). In addition to superior assay sensitivity, the bifurcated microfluidic device provides greater durability and versatility for substrate modification. Using the explosive 2,4,6-trinitrotoluene (TNT) as the model compound in a fluorescence-based displacement immunoassay, we report LODs for TNT at 10 parts-per-trillion (pptr) using a neutravidin-coated biotinylated anti-TNT microfluidic device. Solution to wall interactions were also simulated in COMSOL Multiphysics to understand fluid flow characteristics. Reynolds numbers were calculated to be 0.27–2.45 with a maximum pressure of 1.2 × 10−2 psi. View Full-Text
Keywords: explosives; 2,4,6-trinitrotoluene (TNT), microfluidic; microchannel; immunoassay; fluorescence; antibody explosives; 2,4,6-trinitrotoluene (TNT), microfluidic; microchannel; immunoassay; fluorescence; antibody
Show Figures

Graphical abstract

MDPI and ACS Style

Charles, P.T.; Wadhwa, V.; Kouyate, A.; Mesa-Donado, K.J.; Adams, A.A.; Deschamps, J.R.; Kusterbeck, A.W. A High Aspect Ratio Bifurcated 128-Microchannel Microfluidic Device for Environmental Monitoring of Explosives. Sensors 2018, 18, 1568. https://doi.org/10.3390/s18051568

AMA Style

Charles PT, Wadhwa V, Kouyate A, Mesa-Donado KJ, Adams AA, Deschamps JR, Kusterbeck AW. A High Aspect Ratio Bifurcated 128-Microchannel Microfluidic Device for Environmental Monitoring of Explosives. Sensors. 2018; 18(5):1568. https://doi.org/10.3390/s18051568

Chicago/Turabian Style

Charles, Paul T.; Wadhwa, Varun; Kouyate, Amara; Mesa-Donado, Kelly J.; Adams, Andre A.; Deschamps, Jeffrey R.; Kusterbeck, Anne W. 2018. "A High Aspect Ratio Bifurcated 128-Microchannel Microfluidic Device for Environmental Monitoring of Explosives" Sensors 18, no. 5: 1568. https://doi.org/10.3390/s18051568

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop